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We narrowed to 1,076 results for: IND

Showing: 1021 - 1040 of 1076 results

  2. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Carbenicillin plated over-agar Plate shows several individual colonies and effective selection. 150 µL of 2...Carbenicillin plated over-agar Plate shows less individual colonies than the 1 mg/mL plate and effective...Carbenicillin plated over-agar Plate shows several individual colonies with smaller size than the 1 mg/mL and...

  3. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...purification kit, such as the (Link opens in a new window) QIAquick Gel Extraction Kit . Always follow the...next intended step with the now gel purified DNA. Find more information about DNA quantification here ....in 10μL, make 1.1μg in 11μL. Reference Page Top Index...

  4. Water Bath Protocol

    Type
    Protocol
    ...Find step-by-step instructions on how to use a water bath in the lab....different sizes in the lab. Some water baths contain individual slots for heating up tubes while other water ...manual. If you’re filling up a water bath with individual slots for tubes, remember that when you add the...

  5. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...LB agar plates are frequently used to isolate individual (clonal) colonies of bacteria carrying a specific...copy number refers to the number of copies of an individual plasmid within a single bacterial cell. Large...obtain a higher cell density. Reference Page Top Index...

  6. Centrifugation

    Type
    Protocol
    ...cover the general procedure and features to keep in mind when using any centrifuge. However, each centrifuge...like the G Force calculator (Link opens in a new window) . The centrifuge should come up to speed with ...centrifuge begins to wobble or shake, this could indicate an unbalanced or improperly loaded centrifuge....

  8. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...cloning Golden Gate cloning is not 100% sequence-independent: to avoid undesired digestion, the Type IIS site...

  10. CRISPR in the Clinic

    Type
    Blog Post
    ...cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions and obstacles for future...

  12. Lentivirus Production

    Type
    Protocol
    ...post transfection in individual harvests or a combined harvest where all the individual harvests are pooled...transfection. Test a variety of brands and lots of FBS to find one suitable with your protocols. FBS can be purchased...

  13. PhD Applications After COVID

    Type
    Blog Post
    ...environment and social aspects of the department, you may find out that a program you didn’t know much about could...

  16. Pipetting Protocol

    Type
    Protocol
    ...dispensed. The boxes that the tips come in often indicate a volume range that the tip can hold. This should...pipette you’re using, you will be rotating dials behind the numerical display or the top of the plunger...no liquid should drip from the tip. This could indicate that the tip is not on the pipette properly. Place...

  17. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...recommended plasmid, or test multiple plasmids to find a suitable one. Some labs have reported better results...second peak at a temperature of ~70–75 °C usually indicates the presence of primer dimers which can increase...Methods. 2012 Feb;23(1):18-28. (Link opens in a new window) PMID: 22428977...

  18. Viral Production at Addgene

    Type
    Blog Post
    ...Resources and references Resources at the Addgene blog Find and share AAV Data with Addgene's Data Hub Digital...

  19. AAV Production in HEK293 Cells

    Type
    Protocol
    ... Chambers Cell-Stack (CS5) (Link opens in a new window) (3,180 cm 2 - the same surface area as 21 x T-...transfection. Test a variety of brands and lots of FBS to find one suitable for your protocols. FBS can be purchased...1.8-fold ( Rego et al., 2018 (Link opens in a new window) ). To prepare D1 + 0.1 M sorbitol, add 57 mL of...

  20. Protocol - How to Streak a Plate

    Type
    Protocol
    ...plasmid DNA from it, you will want to isolate an individual clonal population (single colony) of bacteria... proceed to Recovering Plasmid DNA or use the individual colonies for other experiments....
Showing: 1021 - 1040 of 1076 results