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We narrowed to 1,083 results for: IND

Showing: 1021 - 1040 of 1083 results

  5. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ... of a transfected cell line.  Keep these tips in mind: There is not one recommended concentration for...

  6. Lentivirus Production

    Type
    Protocol
    ...post transfection in individual harvests or a combined harvest where all the individual harvests are pooled...transfection. Test a variety of brands and lots of FBS to find one suitable with your protocols. FBS can be purchased...

  7. Pipetting Protocol

    Type
    Protocol
    ...dispensed. The boxes that the tips come in often indicate a volume range that the tip can hold. This should...pipette you’re using, you will be rotating dials behind the numerical display or the top of the plunger...no liquid should drip from the tip. This could indicate that the tip is not on the pipette properly. Place...

  9. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...feasible partially because of viruses—keep reading to find out how!   Neurotransmitters Many canonical...

  11. How Addgene Creates Content

    Type
    Blog Post
    ...make certain videos? In this post, we invite you behind the scenes in our content writing process, and ...

  12. Clear and Accessible Writing

    Type
    Blog Post
    ...Stupid, overwhelmed, discouraged, tired…words that indicated there were many barriers between our audience ...

  15. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...recommended plasmid, or test multiple plasmids to find a suitable one. Some labs have reported better results...second peak at a temperature of ~70–75 °C usually indicates the presence of primer dimers which can increase...Methods. 2012 Feb;23(1):18-28. (Link opens in a new window) PMID: 22428977...

  17. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...cloning Golden Gate cloning is not 100% sequence-independent: to avoid undesired digestion, the Type IIS site...

  19. CRISPR in the Clinic

    Type
    Blog Post
    ...cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions and obstacles for future...
Showing: 1021 - 1040 of 1083 results