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...resources for hiring research staff. And for a more indepth resource for starting your academic lab, download...

... of a transfected cell line.  Keep these tips in mind: There is not one recommended concentration for...

... to protein (260/280) is generally used as an indicator of the purity of DNA samples. These days, many...1.80-2.00). Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two... DNA in a buffered solution. Reference Page Top Index...

...make certain videos? In this post, we invite you behind the scenes in our content writing process, and ...

...proteins interact, since light is only produced if a binding interaction brings the two luciferase components...

...Stupid, overwhelmed, discouraged, tired…words that indicated there were many barriers between our audience ...

...feasible partially because of viruses—keep reading to find out how!   Neurotransmitters Many canonical...

...first!) viral experiment?           Click Here to Find Viral Vector Resources at Addgene! References and...

...advance. Join a LinkedIn group (or make one!) or find the conference's Twitter hashtag. Use social media...

... field with 30 years of basic research standing behind it. Our color changing flower involves two basic...

...Carbenicillin plated over-agar Plate shows several individual colonies and effective selection. 150 µL of 2...Carbenicillin plated over-agar Plate shows less individual colonies than the 1 mg/mL plate and effective...Carbenicillin plated over-agar Plate shows several individual colonies with smaller size than the 1 mg/mL and...

...purification kit, such as the (Link opens in a new window) QIAquick Gel Extraction Kit . Always follow the...next intended step with the now gel purified DNA. Find more information about DNA quantification here ....in 10μL, make 1.1μg in 11μL. Reference Page Top Index...

...Find step-by-step instructions on how to use a water bath in the lab....different sizes in the lab. Some water baths contain individual slots for heating up tubes while other water ...manual. If you’re filling up a water bath with individual slots for tubes, remember that when you add the...

...cloning Golden Gate cloning is not 100% sequence-independent: to avoid undesired digestion, the Type IIS site...

...cover the general procedure and features to keep in mind when using any centrifuge. However, each centrifuge...like the G Force calculator (Link opens in a new window) . The centrifuge should come up to speed with ...centrifuge begins to wobble or shake, this could indicate an unbalanced or improperly loaded centrifuge....

...LB agar plates are frequently used to isolate individual (clonal) colonies of bacteria carrying a specific...solution of your antibiotic. Unless otherwise indicated, the antibiotic powder can be dissolved in dH ...copy number refers to the number of copies of an individual plasmid within a single bacterial cell (Figure...

...troubleshooting viral transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page  ...

...cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions and obstacles for future...

...contexts/locations in the test apparatus and thus individual laser stimulation is essential. In terms of the...

...environment and social aspects of the department, you may find out that a program you didn’t know much about could...