We narrowed to 1,044 results for: STI;

1. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   ...area of the larger vessel to the 10 cm2 dish and adjusting the cell number, plasmid concentration, and experimental...

2. Lab Automation at Addgene

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   Blog Post

   ... of pushing them in one by one. It seems very simplistic and it isn’t electronic but it is one of the ...

3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   Blog Post

   ...experiments that requires modest gene activation. Synergistic Activation Mediator (SAM) Description SAM uses...

4. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   Blog Post

   ... RNPs, AcrIIA4 reduced off-target editing while still allowing on-target editing (Shin et al., 2017). ...

5. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   Blog Post

   ...and its role in gene expression, though this may still unexpectedly affect nearby genes (Figure 1A-C). ...

6. Plasmids 101: Terminators and PolyA signals

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   Blog Post

   ...Multiple copies of the Mason–Pfizer monkey virus constitutive RNA transport element lead to enhanced HIV-1...

7. Plasmids 101: Protein Expression

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   Blog Post

   ...when a protein is expressed through the use of constitutive and inducible promoters. Inducible promoters...

8. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   ... start producing virus within 24 to 48 hours. Harvesting timepoints will depend on your specific virus...

9. Antibodies 101: Flow Compensation

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   Blog Post

   ...your experiment. So, as long as you are able to distinguish your populations from each other on your flow...

10. Plasmids 101: Degron Tags

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    Blog Post

    ...facilitates ubiquitination of the Halo-tagged protein in question, it is targeted to the proteasome for degradation...

11. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post

    ...differentiation) in mouse embryos (Miyatsuka et al.) Distinguish between models for trafficking to the lysosome...

12. Degrading DNA with Cascade-Cas3

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    Blog Post

    ...these systems, though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors...

13. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...such as in the Ultramicroscope.  In addition, adjusting the thickness of the laser sheet and using multi-view...

14. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ... and plan for the future of the team. All while still paying bills and having a life outside my job. The...

15. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ...plasmid-containing bacteria. So… why do some people still use amp? Carb is expensive (relative to amp), and...

16. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...labs. Careful tracking prevents us Addgenies from wasting hours of valuable time searching for samples and...

17. Antibodies 101: Immunoprecipitation

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    Blog Post

    ...sample.  Eluting the target protein for downstream testing Once the non-specific proteins are removed through...

18. How to Write a Scientific Review Article

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    Blog Post

    ...continuity. Also don’t worry if you feel like you don’t still know enough about the topic. Just get as many words...

19. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post

    ...Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression F- ompT lon hsdSB (rB...

20. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    Blog Post

    ...Interactions through Spatial Proximity." Journal of Investigative Dermatology 137.12 (2017): e197-e203. PubMed...