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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 521 - 530 of 1010 results

  1. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ... Research Trust (RSRT) has an ambitious goal of curing Rett Syndrome and Addgene is thrilled to partner...methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson & Bird, 2019) and...neurological deficits observed in Rett Syndrome. Restoring the MECP2 gene by excision of the Stop cassette...have shown exciting and promising results for repairing mutations in models of neurological disease. Klaus...

  2. Hot Plasmids - November 2023

    Type
    Blog Post
    ...phage-assisted continuous evolution and protein engineering to generate seven new prime editors, PE6a–g, ...(2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell,... increase the utility of this technique.  By engineering a light-sensitive LOV domain into the TurboID...mass spectrometry workflow identifies proteins bearing both labels (which had therefore been trafficked...

  3. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...but did not consider them for myself. I started picturing myself in a technical support role only when I...there is a second-line team that takes on cases requiring in-depth analysis, data review, in-house testing...are obtained. Or someone may heat an ELISA plate during incubation steps in order to enhance the OD signals...with other departments such as research and manufacturing, quality assurance, product management, marketing...

  4. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ... not all, of these new experimental species. Discovering ways to culture, propagate, transform, store,...systems. How the EDGE Program promotes scientific sharing The EDGE Program is designed to expand the number..., in general, these labs all include materials sharing in their dissemination plans and in particular,...to mate with and if she decides to raise their offspring or leave them to their own devices. Chytrid fungi...

  5. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...are occurring every year. At Addgene, we want to maintain the highest quality and accuracy during the ...analysis required every week. They are constantly exploring ways to enhance their processes, quality, and ...McQueary References and Resources References Niedringhaus, T. P., Milanova, D., Kerby, M. B., Snyder, ...

  6. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...for the entire translation process. For example, during high growth rate conditions, E. coli preferentially...biosynthetic enzymes may be preferentially charged during amino acid starvation thus resulting in higher ...getting your genes of interest codon optimized by ordering plasmids containing them from Addgene. If a plasmid...that will generally segregate with the cell pellet during purification procedures. Even if you produce a ...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...is a constantly evolving and demanding field, requiring a variety of biological and molecular tools. One... perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive research...be analyzed to understand human disease alleles during and after embryogenesis. Addgene has over 600 plasmids...organism: quick development, a large number of offspring (produces many seeds), self fertilizing, a small...

  8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ... allow cells to become persistently fluorescent during development. Stochastic multicolor labeling systems... tags instead of XFP to optimize it for tissue clearing, a method that makes tissues, such as the brain...visible. Chemical tags are more resistant to tissue clearing methods and can be labeled by synthetic fluorophores...Multicolor Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson...

  9. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...scientific effort. As we like to say at Addgene: sharing speeds science. Here, we’d like to highlight the...Kleinstiver lab also worked on the opposite project: engineering Cas9 variants with a preference for specific ... L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science (New York, ...Custom CRISPR-Cas9 PAM variants via scalable engineering and machine learning. Nature, 643(8071), 539–...

  10. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...tools this could be applied to. When it comes to altering gene expression, CRISPR technologies have gradually...-RNA (miRNA) Short hairpin RNA (shRNA) Short interfering RNA (siRNA) Piwi-interacting RNA (piRNA) miRNA..._module_attribute %}{% end_module_block %}   Interfering in the future While RNAi has retreated a bit ... the background, it remains a solid option for altering the expression of specific mRNAs. shRNAs are a...
Showing: 521 - 530 of 1010 results