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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,076 results for: IND

Showing: 1041 - 1060 of 1076 results

  1. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...NEB’s online tool NEBcloner (Link opens in a new window) , to help guide your reaction buffer selection...errors may occur. Addgene makes no warranty of any kind regarding the contents of any literature. Literature...IS” “AS AVAILABLE” basis without warranty of any kind either expressed or implied, including but not limited...

  2. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...anneal to a specific region of the insert. You can find Addgene's sequencing results by clicking on the ...unreliable. Although your sequencing results may indicate bases at specific locations, by looking at the...

  3. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ... . The pattern of the fragments on the gel can indicate if the plasmid contains the expected size insert... or restriction enzyme -based cloning to test individual clones before use of more expensive forms of ...

  4. Fluorescence Titering Assay

    Type
    Protocol
    ...transfection. Test a variety of brands and lots of FBS to find one suitable with your protocols. FBS can be purchased...Check the cells for mycoplasma (Link opens in a new window) regularly Do not over- or under-grow your cells...

  5. Immunocytochemistry

    Type
    Protocol
    ...4% Paraformaldehyde 5 mg/mL 4′,6-diamidino-2-phenylindole (DAPI) Bovine serum albumin (BSA) Triton X-100...may need to try a variety of fixation methods to find the best conditions for your target. The optimal...

  6. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... μl of lab stock solution per 100 mL gel). EtBr binds to the DNA and allows you to visualize the DNA under...10 μL, make 1.1 μg in 11 μL. Reference Page Top Index...

  7. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...mobility of the bands (sample AR0016 in Figure 1) indicates that the samples may not have been processed correctly... Image Analysis Use ImageJ (Link opens in a new window) or a similar photo software to determine the relative...

  8. Western Blot

    Type
    Protocol
    ... 2 h at RT but may result in more non-specific binding. Wash the membrane 3x for 5 min in 1X TBST at RT...You may need to try a variety of lysis buffers to find the best for your target. The optimal concentration...

  9. Kit Free RNA Extraction

    Type
    Protocol
    ...opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new window) TRIzol® User ...

  10. Lentivirus ddPCR Titration

    Type
    Protocol
    ...the next dilution. Repeat for all dilutions as indicated in the chart below. Dilution Factor Virus Volume...hgtb.2017.198. PMID: 29378428 (Link opens in a new window) ....

  15. Weighing Reagents Protocol

    Type
    Protocol
    ...in the photo above has a capacity of 200 g, as indicated by the number above the "Zero" button. Before ...

  16. General Transfection

    Type
    Protocol
    ...transfection. Test a variety of brands and lots of FBS to find one suitable with your protocols. FBS can be purchased...

  18. Using a Light Microscope Protocol

    Type
    Protocol
    ...practice to start with the lowest power objective to find your sample. Place your slide (or other sample type...

  19. AAV ddPCR Titration

    Type
    Protocol
    ...publication Lock et al. (2014) (Link opens in a new window) . Before Starting To reduce the risk of contaminating...

  20. Antibody Guide

    Type
    Guide
    ...structure are responsible for binding the antigen, and are called the antigen binding fragments (Fab). The tips...recognizing and binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on...single individual, giving a high probability that an antibody will be able to recognize and bind to any... and binds to the protein of interest. Signal strength is determined by how many antibodies bind the protein...detection antibody is added and binds to the bound protein. If using the indirect sandwich method, a conjugated...? Will you be using a direct or indirect detection method? If indirect, do you already have secondary ...tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as part ...
Showing: 1041 - 1060 of 1076 results