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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,083 results for: IND

Showing: 1041 - 1060 of 1083 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ... Chambers Cell-Stack (CS5) (Link opens in a new window) (3,180 cm 2 - the same surface area as 21 x T-...transfection. Test a variety of brands and lots of FBS to find one suitable for your protocols. FBS can be purchased...1.8-fold ( Rego et al., 2018 (Link opens in a new window) ). To prepare D1 + 0.1 M sorbitol, add 57 mL of...

  3. PhD Applications After COVID

    Type
    Blog Post
    ...environment and social aspects of the department, you may find out that a program you didn’t know much about could...

  5. Viral Production at Addgene

    Type
    Blog Post
    ...Resources and references Resources at the Addgene blog Find and share AAV Data with Addgene's Data Hub Digital...

  6. Protocol - How to Streak a Plate

    Type
    Protocol
    ...plasmid DNA from it, you will want to isolate an individual clonal population (single colony) of bacteria... proceed to Recovering Plasmid DNA or use the individual colonies for other experiments....

  7. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...NEB’s online tool NEBcloner (Link opens in a new window) , to help guide your reaction buffer selection...errors may occur. Addgene makes no warranty of any kind regarding the contents of any literature. Literature...IS” “AS AVAILABLE” basis without warranty of any kind either expressed or implied, including but not limited...

  8. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...anneal to a specific region of the insert. You can find Addgene's sequencing results by clicking on the ...unreliable. Although your sequencing results may indicate bases at specific locations, by looking at the...

  9. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ... . The pattern of the fragments on the gel can indicate if the plasmid contains the expected size insert... or restriction enzyme -based cloning to test individual clones before use of more expensive forms of ...

  10. Fluorescence Titering Assay

    Type
    Protocol
    ...transfection. Test a variety of brands and lots of FBS to find one suitable with your protocols. FBS can be purchased...Check the cells for mycoplasma (Link opens in a new window) regularly Do not over- or under-grow your cells...

  11. Immunocytochemistry

    Type
    Protocol
    ...4% Paraformaldehyde 5 mg/mL 4′,6-diamidino-2-phenylindole (DAPI) Bovine serum albumin (BSA) Triton X-100...may need to try a variety of fixation methods to find the best conditions for your target. The optimal...

  12. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... μl of lab stock solution per 100 mL gel). EtBr binds to the DNA and allows you to visualize the DNA under...10 μL, make 1.1 μg in 11 μL. Reference Page Top Index...

  13. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...mobility of the bands (sample AR0016 in Figure 1) indicates that the samples may not have been processed correctly... Image Analysis Use ImageJ (Link opens in a new window) or a similar photo software to determine the relative...

  14. Western Blot

    Type
    Protocol
    ... 2 h at RT but may result in more non-specific binding. Wash the membrane 3x for 5 min in 1X TBST at RT...You may need to try a variety of lysis buffers to find the best for your target. The optimal concentration...

  15. Kit Free RNA Extraction

    Type
    Protocol
    ...opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new window) TRIzol® User ...

  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ...the next dilution. Repeat for all dilutions as indicated in the chart below. Dilution Factor Virus Volume...hgtb.2017.198. PMID: 29378428 (Link opens in a new window) ....
Showing: 1041 - 1060 of 1083 results