We narrowed to 1,075 results for: TOR
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TypeBlog Post.... The pDonor plasmid pRE57-Ter with multiple terminators can serve to interrupt genes (or gene clusters...
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Lab to Office Culture Shock
TypeBlog Post...industry, nonprofit organization, law office, journal editor, etc.--so everyone’s experience will be a little... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...given therapeutic approach brings to patients, doctors, and insurance providers, and is crucial to how... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...clone the PCR products into a pBluescript cloning vector and sequence the resulting plasmid. It can take... -
CRISPR 101: Validating Your Genome Edit
TypeBlog Post...performing a single base pair edit with a prime editor. What next? When generating a knock-in mutation... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...currently a research scientist in the Department of Laboratory Medicine at the University of Washington. She... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...to drive cell type-specific delivery of a viral vector (Strebinger et al., 2023). All this flexibility... -
Protocol - How to Streak a Plate
TypeProtocol...loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar plate (with appropriate...resistance and your initials. Labeling within a laboratory setting is important for organization, and it... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol...spreading of antibiotic makes it easy for an investigator to conveniently plate and select transformed...spreading Bunsen burner (or other small flame source) Incubator Reagents 6 cm diameter LB/agar plates without ... -
Water Bath Protocol
TypeProtocol... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to .... Water baths can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room... -
Deep Dive: qPCR
TypeBlog Post...19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais ... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...a blank chart to your spreadsheet. In the Chart Editor , select the Chart Type drop down menu and select...calculate this for you as follows: In the Chart Editor , select the Customize tab. Select the Series drop... -
Western Blot
TypeProtocol...microcentrifuge tube. Use the lysate immediately or store at -80 °C until ready to use. Section 2: Determine...the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the plastic tray. Place... -
DNA Quantification
TypeProtocol... to protein (260/280) is generally used as an indicator of the purity of DNA samples. These days, many... -
Protocol - How to Perform Sequence Analysis
TypeProtocol...important for your experiments. There are a number of factors to consider when selecting sequencing primers, ... -
Weighing Reagents Protocol
TypeProtocol... reagents for a stock mixture is an essential laboratory technique, as imprecise measurements can affect... -
Gibson Assembly Protocol
TypeProtocol...especially useful for introducing promoters, terminators, and other short sequences into the assembly ... -
Protocol - How to Run an Agarose Gel
TypeProtocol... After all of the sample is unloaded, push the pipettor to the second stop and carefully raise the pipette... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...curve or dilute the unknown sample (don’t forget to factor in that dilution when calculating the original ... -
Adenovirus Guide
TypeGuide...Viral Vectors Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...adenoviral (rAdV) vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus...Generations First-generation rAdV vectors The first generation of rAdV vectors was developed by removing the...-generation rAdV vectors. Created with BioRender.com. Second-generation rAdV vectors Second-generation...Second-generation rAdV vectors contain the genomic deletions of first-generation rAdV vectors, as well as the deletion...