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We narrowed to 1,010 results for: RING;

Showing: 531 - 540 of 1010 results

  1. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...tools this could be applied to. When it comes to altering gene expression, CRISPR technologies have gradually...-RNA (miRNA) Short hairpin RNA (shRNA) Short interfering RNA (siRNA) Piwi-interacting RNA (piRNA) miRNA..._module_attribute %}{% end_module_block %}   Interfering in the future While RNAi has retreated a bit ... the background, it remains a solid option for altering the expression of specific mRNAs. shRNAs are a...

  2. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...biotin-streptavidin interaction (Figure 1a). This interaction brings the fluorophore in proximity to the target marker... used. These dyes are incorporated into the DNA during DNA synthesis and then diluted with subsequent ...negative live cells can still be easily gated on during the analysis. Conclusion Congratulations! You made...intracellular labeling protocol is necessary, requiring chemical treatment of the cells after surface ...

  3. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...), and in SNP analysis (to introduce naturally occuring SNPs in a plasmid context). The technique is also...sequence critical for Cas9 cleavage), thereby rendering the resulting construct resistant to Cas9 induced... successfully anneal to the plasmid of interest during the PCR reaction. Ideally, your primers should ...’ll want to steer clear of these strains when preparing the plasmid template for site directed mutagenesis...

  4. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...assembly work? The required homology between neighboring fragments can be created via PCR amplification...including regions of homology to the vector or neighboring fragment. Then you would amplify the fragments...required single-stranded annealing and priming of neighboring assembly fragments. Gibson assembly is commonly...modular overlap-directed assembly with linkers) which brings modularity to overlap-directed methods (Casini ...

  5. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...helpful from a biological standpoint, it makes delivering AAVs to the brain difficult. Traditionally, delivery...and/or its intermediate engineered precursor. Considering systemic capsids for your research? While systemic.../cJ and CBA/J mice. AAV1-X1 was developed by transferring the 7-mer peptide region from variable region...Fox AS. (2023). Advances in AAV technology for delivering genetically encoded cargo to the nonhuman primate...Bloom, J. M., Seed, C., & Deverman, B. E. (2019). Delivering genes across the blood-brain barrier: LY6A, a...AAV vectors to the central nervous system by engineering capsid–receptor interactions that enable crossing...

  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...An off-target rate of 5% is far too high when considering human patients and the diseases associated with...off-target frequency is (more on this below). Lowering off-target events There are several strategies...compatible with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you’ll want ...which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific...

  7. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...recorded plasmid multimerization in the naturally occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968...whole plasmid sequencing of our plasmid samples during our quality control process. Since MiSeq involves...D, Berg DE. pBR322-derived multicopy plasmids harboring large inserts are often dimers in Escherichia ...Guttman A, Thompson L. Vaccine Plasmid Topology Monitoring by Capillary Gel Electrophoresis. Curr Mol Med...

  8. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...minimize recombination that are typically employed during viral plasmid amplification (Fischer, KB. 2019)... showing the mechanism of spontaneous reversion during AAV plasmid amplification. On the left homologous...  Some classes of neurons transiently express TH during development or have low levels of TH mRNA without...time, frustration, and funds in the long-run by ensuring you are minimizing any off-target expression that...

  9. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...insertions were positive or negative selected for during the experiment (Sandoval-Villegas et al., 2021)...Targeting) system. INTEGRATE is based on a naturally occurring Tn7 transposon found in Vibrio cholerae that encodes...Sleeping Beauty, piggyBac and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084...about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty DNA transposon...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...the five is different. To identify significant pairings, additional tests, referred to as post-hoc analysis...temperature, humidity, sunlight exposure time, and watering volume on total crop production (though you would... error is the probability of a false positive occurring when many comparisons are performed. For example...drawbacks to these tests as they can be quite stringent if many samples are assessed. However, if many...
Showing: 531 - 540 of 1010 results