We narrowed to 872 results for: cel

...of viruses used to deliver CRISPR components to cells or organisms under study. In the past we’ve left... they want to do is to quickly generate a single cell line and proceed to use it for experiments.” - ...screen for genes involved in a wide variety of cellular processes. While these are widely requested and...representation in the viral prep, infect target cells, and, finally, conduct the screen. To help alleviate...deliver gRNA and therefore, must be used to infect a cell line already expressing Cas9. As John Doench (Broad...

...Awardees: Xin Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their...reliably detect HMOs. Fatima’s first design is a whole cell biosensor with two components - an enzyme that breaks...proposes using this sensor to detect HMOs found on cellular surfaces. After incubating the sensor with bacteria...binding results in an increase in fluorescence of cells that actively produce the HMOs. Fatima has demonstrated...out Fatima's recent publication on her HMO work in Cell Chemical Biology! Additional Resources on the Addgene...

... between Cas9 (which causes the breaks) and host cell DNA repair factors (which repair those breaks) makes...example, correcting the allele that causes sickle cell anemia. However, HDR occurs much less frequently...a structure could drive sequence replacement in cells. Targeting three dCas9 molecules to a precisely ...functional Cas9 protein and the targeting guide RNA into cells by electroporation. Results from our lab and others...Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins...

...check its subcellular location and solubility. If it’s membrane-bound or located in a subcellular compartment...reagent. Be aware that this approach requires excellent attention to detail, since you’ll end up with ...question before running one.  Good on gradients? Excellent! Now, let’s head west, intrepid reader! Protein...strengths. If that’s not working, consider using a subcellular fractionation kit or a lysing protocol developed...to see if one works better than another. For nitrocellose membranes, you can even give gelatin a shot....strength Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough...

...editor in mammalian cells Not all Cas13d proteins are functional in mammalian cells, but Konermann et al...make it possible to target essentially any RNA in a cell. Since catalytically dead dCasRx maintains its RNA-binding... with RNA-Targeting Type VI-D CRISPR Effectors.” Cell (2018) pii: S0092-8674(18)30207-1. PubMed PMID: ... a WYL-Domain-Containing Accessory Protein.” Mol Cell. (2018) pii: S1097-2765(18)30173-4. PubMed PMID:...

...to studying membranes, organelles, and other subcellular compartments is via proteomics--the study of ...interaction networks of specific proteins in living cells. Until recently, the enzymes most frequently used...but requires H2O2, which can be toxic in living cells. BioID was derived from biotin ligase and uses far...Alexander Green lab found a solution: a low-cost, cell-free detection assay that can be completed without...family, known to be involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu group...

...Drosophila cell line, I’ve studied a transcription factor important for T cell differentiation (a cell type ...regeneration and homeostasis in the Drosophila midgut. Cell. 137, 1343–55 (2009). PubMed PMID: 19563763. PubMed...et al. Digoxin and its derivatives suppress TH17 cell differentiation by antagonizing RORγt activity. ...

... Process ChIP can be done for either tissues or cells. The process is very similar for both, though tissue...fragmenting the DNA, spin down the samples to remove cell debris, which will collect in a pellet. The supernatant..., these are mostly fairly standard molecular and cell biology techniques. What’s all the fuss? Well, ChIP...will also have to be optimized for both tissue or cell type, amount of protein, and desired fragment length...right!  Fig. 2: Goldilocks is actually celebrating a successful ChIP experiment! Image courtesy ...

...toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(...advantageous to instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing...tRNAs are among the most abundant molecules in a cell, this system is clearly also robust enough to ensure...54(4):698-710. doi: 10.1016/j.molcel.2014.04.022. Epub 2014 May 15. PubMed.  Find the plasmids from this...

...system, antibodies are produced by B cells. Each individual B cell produces antibodies that all recognize...also all the same isotype. But together, all the B cell clones in the immune system make different isotypes...antibodies starts with selecting just one of these B cell clones for further antibody production, polyclonal... blood from the immunized animal. The red blood cells and the serum are separated, and the serum - which...