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We narrowed to 954 results for: COMP

Showing: 551 - 560 of 954 results
  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...lacY1 proA2 rpsL20 xyl-5 leu mtl-1 Stbl4   Electrocompetent cells for cloning and storage of unstable ...XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of large plasmids, ligated...tables are by no means exhaustive! For a more comprehensive list and additional information, visit OpenWetWare's...of Bacterial Expression Systems. Check out our companion post describing protein expression strains, where...
  2. Weighing Reagents Protocol

    Type
    Protocol
    ...reagents is important to ensure the accurate composition of your solutions and buffers. Now that you have...
  3. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...certain Cas enzymes are less compatible with the bulkiness that accompanies mispairing of bases. To reduce...unnecessary for many applications, it is the only comprehensive method available. Targeted sequencing approaches...must be quantified slightly differently. For a comprehensive approach, RNA-seq would be an appropriate method...
  4. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...helps all viewers, especially if you have some complex patterns with several overlapping targets to interpret...you add the secondary antibody, it should get completely washed away and leave no signal. If you see lots...samples where you label only one target in each. Compare these individually-labeled samples with the results...deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National Academy of...
  5. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...equipment on a lab bench. The best part? Less complicated math. Bioengineering is a field that has been...achieve whatever goal you’re aiming for. When you complete each cycle, your best-performing mutant is usually...or predicted structure of your antibody-antigen complex as a template. This structural framework then guides... a plate-based format, making it suitable for comparing relative affinities across mutants. Cell surface...
  6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...could avoid potential off target artifacts by comparing the phenotypes of multiple gRNAs. Targeting different...lanes 0.3 % 3.0, Figure 5, lane 2) is faint, so comparing it to the undigested control (Figure 4, lane 0...instead of agarose gives much sharper bands. I compared two MOIs of gRNA transduction to see if that had...testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months to knockout a...
  7. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    ...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round data.... Bobae An will host a Q&A session after the competition, where you can get all your burning AAV questions...
Showing: 551 - 560 of 954 results