We narrowed to 872 results for: cel

...to detect dead or dying cells so that you can base your analysis on living cells only (for example, propidium...This tends to be most problematic when staining cells or tissues that are the same species as your primary...propidium iodide only stains dead cells). Compensation controls address the spectral overlap that occurs...Quantitative Fluorescence Microscopy.” Journal of Cell Biology, vol. 185, no. 7, Rockefeller University...

...immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where...know if they co-localize at the same place in the cell? Or you know that the presence of several biomarkers...tissue sections, methanol- or paraformaldehyde-fixed cell samples, or other preparations. Sequential IF For...hippocampus, or counting the number of puncta in each cell. When making figures for a paper or presentation...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

...use electro-competent cells instead of the more common chemically-competent cells. You should perform, ... manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-...1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total...colonies, you might want to use higher competency cells. Additionally, if your final product is going to...

...Mammalian cells provide scientists with the means to study biological processes on the cellular level instead...viral lots. Aligning with Addgene’s mission to “accelerate research and discovery by improving access to...with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly ...

...institutions in Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh...developing novel systems for bioconversion of cellulosic biomass to useful products under Professor Christopher...researchers in developing regions and thereby accelerate their research. Kwabena came across the plasmid...biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's plans for...

...high-throughput screening techniques such as ELISA and cell surface display are common. ELISA enables quantitative...for comparing relative affinities across mutants. Cell surface display systems, such as bacterial, yeast...presentation of your mutant libraries on the surface of cells. This enables direct screening via flow cytometry...provide recombinant antibodies that target families of cell surface and secreted proteins (glypicans, integrins...therapies and improve their delivery methods to various cells. The wealth of smaller engineered antibody fragments...

...temperature incompatible to genome editing in human cells. The Feng Zhang lab identified a Cas12b from Bacillus...can block Cas12a-mediated genome editing in human cells. Read the paper in Science Find the anti-CRISPR...anti-CRISPR expression plasmids CRISPR-based method for cell lineage tracing Valcyrie is a CRISPR-based marker...marker excision method for marking distinct cell clones developed by the Michalis Averof lab. It’s an alternative...

... vectors and genetically target them to specific cell populations. They demonstrated use of the LITEs ...non-invasively, its use is not restricted to cultured cells but extends to freely moving animals, as established...novel mode of optogenetic control of endogenous cellular processes." The LITE system can now be applied...no means limited to particular model organisms or cell types. “This tool is really flexible and modular...

...your life that may end with little more than an Excel spreadsheet of random numbers that’ll leave you,...your assay will scale. Or you don’t have enough cells to execute genome-wide perturbations. So, while ...lentiGuide expressing only an gRNA, for use with cells that already have Cas9). Each library consists of...be useful when screening for phenotypes, such as cell-non-autonomous effects, that do not lend themselves...

... GFP, enabling you to select cells with high GFP expression. These cells have the highest levels of Cas9... on the same plasmid, you’ll make sure that each cell that gets the plasmid contains all of the desired...gRNA, which causes hypopigmentation, thus marking cells that have undergone genomic modification. In this..., et al. “Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system.” Scientific...