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We narrowed to 878 results for: cel

Showing: 581 - 590 of 878 results

  1. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ... have been extracted from a cell, instead of when it is attached to a cell membrane.  Picking the Right...epitopes that are available on the outside of the cell. The same protein may have different epitopes available...need to validate on denatured proteins or on live cells. But for others, you may need to validate that it...

  2. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...the vacuole in yeast or the lysosome in mammalian cells. This decrease in pH quenches SEP but not DsRed,...acidity, this biosensor can’t be used with fixed cells. Find the mitochondria-targeted Keima variant at...Levine. "Methods in mammalian autophagy research." Cell 140.3 (2010): 313-326. PubMed PMID: 20144757. PubMedCentral...measurements of macroautophagy and mitophagy in mammalian cells using a single fluorescent biosensor." Scientific...

  3. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...testing at Addgene meaning they come ready made to accelerate your research.   Why distribute virus? Plasmid...This service will further enable scientists and accelerate research." What viral preps are available... can be used to deliver fluorescent proteins to cells as determined by the serotype. You can use these...determine if your AAVs are getting to the right cells using your particular delivery technique and conditions...

  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ... be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In contrast...unable to show TtAgo-mediated cleavage in mammalian cells, likely due to high temperature requirements. To...plasmid and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9...

  5. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...Nanobody toolkit to monitor retrograde transport at the cell surface Article contributed by Marcy Patrick  Listen...nanobodies to study retrograde transport from the cell surface. Their toolkit includes a set of plasmids...new technologies to quantify proteins in tissues, cells, and biofluids. Recently, Sam Myers, a Scientist...an sgRNA expressed from a separate plasmid. When cells are treated with biotin–phenol and H2O2, APEX2 labels...

  6. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...R-factors could be transferred between bacteria via cell-to-cell contact; however, scientists noted that, unlike...cytoplasmic factors that could be transferred between cells. At the time, these extranuclear agents of heredity...PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary Symbiosis" Physiological...

  7. The Pleiades Promoter Project

    Type
    Collection
    ...therapies for brain disorders by enabling region- and cell-specific gene-delivery in the mouse brain through...

  8. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...negative bacteria is due to differences in cell wall composition. Cell wall structure Gram positive bacteria...complex within the peptidoglycan layer, staining the cell purple. When the alcohol is added, it dehydrates...Silhavy, TJ., Kahne, D. & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414...

  9. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ... glutamate release from neurons and other brain cells in vivo. Glutamate plays a variety of roles in synaptic...sensor to the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD,...promoter, FLEXed for expression in Cre-expressing cells hSynap: Human Synapsin-1 promoter. Good for neuronal...promoter, FLEXed for expression in Cre-expressing cells. hSynap-FLP: Synapsin-1 promoter, FLP-dependent ...

  10. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ... results, but also confirm expression in target cells. While the various AAV serotypes can be used to ...Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in the brain, and holds promise... systems such as Cre-lox allow for more accurate cell and tissue targeting. While promoters and AAV serotypes...allowing you to further restrict gene activation to cells expressing both Cre and GFP. The GFP dependent FLPo...
Showing: 581 - 590 of 878 results