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We narrowed to 1,011 results for: RING;

Showing: 51 - 60 of 1011 results

  1. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...talk, which focused on the history of genome engineering from ZFNs through TALENs and CRISPR-Cas nucleases...huge impact on this field. Advances in genome engineering & CRISPR technology That afternoon's workshop...workshop, entitled "Advances in Genome Engineering", brought us 8 short talks on every conceivable way of examining...more parts-oriented synthetic biology. I've been answering biology customer service questions for nearly ...scientists are tackling this type of genetic engineering is a testament to how amazing the downstream ...downstream payoff could be. Though I attended an engineering school as an undergraduate, I never fooled myself ... The talks we heard Monday night represented engineering first and foremost, from the custom software ...

  2. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...jwoodgett.     More Discussions About Data Sharing: PLOS Data Sharing Policy Discussion PLOS’ New Data Policy...this year when it announced its data access and sharing policy. Since early March, the open access publisher...reasonable. PLOS has since clarified their data sharing policy and recently announced that of the 16,000...funding agencies (e.g. Wellcome Trust, NIH Data Sharing Policies, etc.) and is founded on the principle...data should be included? We should recognize that storing data in a sharable form is indeed a significant...Publishers have also contributed to this issue by requiring ever more condensed methods descriptions, legends...existing, published data. The importance of data sharing The reason this topic is important to my lab is...

  3. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ... take a moment to review what exactly you are measuring and how to interpret your results.  Quick note...Purification reagent contaminants The reagents used during nucleic acid purification can leave behind a variety... more considerations and other approaches for measuring DNA quantity and quality than we have covered ...Resources on Addgene.org DNA Quantification Protocol Recovering Plasmid DNA from Bacterial Culture Protocol ...

  4. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...by Benoit Giquel. CRISPR has taken the genome engineering world by storm owing to its ease of use and utility...Waltz, 2016), the potential of CRISPR for genome engineering in plants is also being realized. There are a...approaches, these techniques are cumbersome, often requiring several rounds of selection to isolate plants ...plants with the phenotype of interest. Genome engineering, on the other hand, allows for targeted modification...will present a general overview of plant genome engineering using CRISPR, highlight the specific modifications...plants, and outline the various plant genome engineering tools that are available to academic researchers... Addgene. CRISPR components for plant genome engineering CRISPR can be used to knockout, activate or repress...

  5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...Collection under the insect expression system or by filtering by fly as species of gene. Find Gal4 and UAS plasmids...select for transformed bacteria, but you might be wondering, “How do I know which of my flies have the desired...about Using Cre in Drosophila Read Other Genome Engineering Posts Use FLEx Vectors for Site-Specific Recombination... genome engineering...

  6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...interference, these enzymes can knock down RNA without altering the genome, but Cas13s have higher on-target specificity...enzymes are the latest tools in the transcriptome engineering toolbox. How were Cas13d enzymes discovered?...MAPT, the gene encoding dementia-associated tau, delivering dCasRx and a 3-spacer array targeting the MAPT... tell how large the genome and transcriptome engineering toolbox will be. It is, however, certain that...that the impact of CRISPR scientific sharing will continue to grow, and we at Addgene appreciate our depositors...References Konermann, Silvana, et al. “Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors...  Mary Gearing...

  7. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...and its use as a genetic engineering tool is frequently called recombineering - short for homologous recombination-mediated...components of the Lambda Red recombineering system The lambda red recombineering system has three components...for generating dsDNA recombineering substrates to avoid errors introduced during PCR. ssDNA Substrate ...substrates can be generated by ordering synthetic oligonucleotides or denaturing short PCR products. Either..., Court DL. Recombineering: A Homologous Recombination-Based Method of Genetic Engineering. Nature protocols...Costantino, N. and Court, D. L. 2014. Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination...method that can be used for cloning or genome engineering and is based on homologous recombination. It ...

  8. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ... developed during the COVID-19 pandemic. Our mission to accelerate research through sharing reagents has...2021.03.10.434840 Tsang J, LaManna CM (2020) Open Sharing During COVID-19: CRISPR-Based Detection Tools. The...detect SARS-CoV-2 nucleic acids. Mammalian targets During the 2003 SARS outbreak, Hyeryun Choe’s lab identified...-CoV-2 entry into human lung epithelial cells (Biering et al., 2021). In their search, they found mucins...inhibitors of SARS-CoV-2 entry and become upregulated during viral infection.  Expression of SARS-CoV-2 viral...academic and industry scientists through plasmid sharing and more. As you’ve read above, some of these plasmids....     References and resources References Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla...

  10. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ... learning. 1. Create a peer mentoring group We don’t all get the mentoring support we need, and most of...Own Peer Mentoring Group: A How-To Guide for Scientists.   Once in-person, peer-mentoring groups ...use more mentors to listen, guide and support us during training and throughout our careers. An easy way...goals.  I’ve been surprised to see that virtual mentoring groups seem to be as successful as in person groups...? You could do a talk virtually for your peer mentoring group (see above). However, now that we are all...present, I’m asked what Addgene “looks for” when hiring a scientist. Of course, we have different criteria...
Showing: 51 - 60 of 1011 results