All in a Twist: dsRNA
Type
Blog Post
...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...
We narrowed to 402 results for: MUT
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It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...domain of a Ciona intestinalis voltage sensor and a mutated version of the pHLuorin fluorescent protein. This... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...light-sensitive protein QuasAr2 and systematically mutated it. Using a robotic picking approach to select ... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post... the protein. To monitor autophagy, the Pho8Δ60 mutant protein is expressed. Pho8Δ60 localizes to the ... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating... -
Plasmids 101: Cre-lox
TypeBlog Post...potentially recombine. To account for this, alternate mutant versions of the loxP site have been created, which... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...promoter of the 5’ long terminal repeat (LTR) is mutated in third generation systems such that it no longer... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...titration consider using a reverse transcriptase mutant in a separate control reaction. This will allow... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab have allowed... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser... -
Pooled Library Amplifications
TypeBlog Post...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus... -
3D Printing Meets CRISPR Cas9
TypeBlog Post... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post... gene expression. Its catalytic domains can be mutated to inactivate the protein’s nucleolytic capability... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post... and Citrine) can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and ... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...depositor has indicated that there is a particular mutation found within the gene/insert, this information... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...should also note that IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...tools, double dimerization GFPs, and circular permutation GFPs. Hopefully we will have new tools for plant... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...gates." Science. 340, 599-603 (3 May 2013). Vivek Mutalik et al. "Precise and reliable gene expression via... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...autoplay=False, hidden_controls=False, loop=False, muted=False, width='600', height='189', player_id='31668202421... -
Tips for arabidopsis transformation
TypeBlog Post...and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...Targeted Evolution. In this technique, a pool of mutant AAV capsid (cap) expressing plasmids is generated... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...namely that they can cause cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post...lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now you’re ... -
Lentiviral Vector Uses and Overview
TypeBlog Post...transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package itself due... -
CRISPR Antimicrobials
TypeBlog Post...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization... -
Plasmids 101: Protein tags
TypeBlog Post...column runs. The TEV protease itself, with various mutations used to increase its stability activity, can be... -
Hot Plasmids - August 2020
TypeBlog Post...) and GRAB_NE1m (medium affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers... -
Plasmids 101: Codon usage bias
TypeBlog Post...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...leverage another organization’s expertise to our mutual benefit. Our partnership with SnapGene, for example... -
Transferable Skills Guide: Creativity
TypeBlog Post...Gather the resources you’ll need ahead of time and mute your notifications during. (Boyes, 2019) If you’... -
Viral Production at Addgene
TypeBlog Post...for viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...transduction of cells, resulting in cell population mutated by the viral pooled library. These cells can then... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post... This is to avoid the chances of insertional mutagenesis, which is the integration of transgenes into ... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...plasmid replication control that are not necessarily mutually exclusive. In the first, the replication initiation... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...2006) Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org... -
Lab to Office Culture Shock
TypeBlog Post...everyone has a voice and the group can come to a mutual agreement is an amazing thing to be a part of and... -
CRISPR Activation: A Practical Guide
TypeBlog Post...upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity to a ... -
The Challenges of Cell Culture
TypeBlog Post...between cells lines, as this can be dependent on the mutation rate of the cell line, but it is broadly recommended...