We narrowed to 416 results for: MUT

1. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively...

2. RaPID Detection of RNA-protein Interactions

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   ...early-onset cataracts, is characterized by point mutations in the Iron Response Element (IRE) RNA motif of...

3. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   ... the protein. To monitor autophagy, the Pho8Δ60 mutant protein is expressed. Pho8Δ60 localizes to the ...

4. Plasmids 101: The protein expression toolbox

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   ... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...

5. All in a Twist: dsRNA

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   ...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...

6. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...light-sensitive protein QuasAr2 and systematically mutated it. Using a robotic picking approach to select ...

7. CRISPR 101: Cas9 vs. The Other Cas(s)

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   ...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...

8. Antibodies 101: Affinity Reagents

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   ...fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used for assembling ...

9. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ...domain of a Ciona intestinalis voltage sensor and a mutated version of the pHLuorin fluorescent protein. This...

10. New Tool for Lineage Tracing: The ClonTracer Library

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    ...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...

11. Plasmids 101: Golden Gate Cloning

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    ...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

12. Plasmids 101: Cre-lox

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    ...potentially recombine. To account for this, alternate mutant versions of the loxP site have been created, which...

13. Overcoming the Challenges of Lentiviral Production

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    ...promoter of the 5’ long terminal repeat (LTR) is mutated in third generation systems such that it no longer...

14. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...

15. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ...

16. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

17. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    ...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...

18. Mouse Modeling, Part 1: Genetically Engineered Mice

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    ...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an...

19. Tips for Titering Your Lentiviral Preps

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    ...titration consider using a reverse transcriptase mutant in a separate control reaction. This will allow...

20. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    ...seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab have allowed...

21. The Materials Science of Optogenetics Experiments

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    ...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser...

22. 3D Printing Meets CRISPR Cas9

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    ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...

23. Truncated gRNAs for Regulating Gene Expression

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    ... gene expression. Its catalytic domains can be mutated to inactivate the protein’s nucleolytic capability...

24. Plasmids 101: Knockout/Knock-In Plasmids

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    ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

25. Degrading DNA with Cascade-Cas3

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    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...

26. Pooled Library Amplifications

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    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

27. Google Forums Round Up: First Impressions of NgAgo

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    ...wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can...

28. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ... and Citrine) can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and ...

29. Anatomy of a Plasmid Page at Addgene

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    ...depositor has indicated that there is a particular mutation found within the gene/insert, this information...

30. Plasmids 101: Multicistronic Vectors

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    ...should also note that IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized...

31. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...tools, double dimerization GFPs, and circular permutation GFPs. Hopefully we will have new tools for plant...

32. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ...gates." Science. 340, 599-603 (3 May 2013). Vivek Mutalik et al. "Precise and reliable gene expression via...

33. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now you’re ...

34. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...Targeted Evolution. In this technique, a pool of mutant AAV capsid (cap) expressing plasmids is generated...

35. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    ...autoplay=False, hidden_controls=False, loop=False, muted=False, width='600', height='189', player_id='31668202421...

36. Viral Vectors 101: The Retroviral Lifecycle

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    ...namely that they can cause cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular...

37. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...

38. Tips for arabidopsis transformation

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    ...and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral...

39. Lentiviral Vector Uses and Overview

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    ...transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package itself due...

40. CRISPR Antimicrobials

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    ...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

41. Plasmids 101: Protein tags

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    ...column runs. The TEV protease itself, with various mutations used to increase its stability activity, can be...

42. Plasmids 101: Codon usage bias

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    ...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ...

43. Transferable Skills Guide: External Collaborations

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    ...leverage another organization’s expertise to our mutual benefit.  Our partnership with SnapGene, for example...

44. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ... This is to avoid the chances of insertional mutagenesis, which is the integration of transgenes into ...

45. Hot Plasmids - August 2020

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    ...) and GRAB_NE1m (medium affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene...

46. Plasmids 101: Plasmid Incompatibility

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    ...plasmid replication control that are not necessarily mutually exclusive. In the first, the replication initiation...

47. Viral Vectors 101: Preparing Pooled Libraries

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    ...transduction of cells, resulting in cell population mutated by the viral pooled library. These cells can then...

48. Transferable Skills Guide: Creativity

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    ...Gather the resources you’ll need ahead of time and mute your notifications during. (Boyes, 2019) If you’...

49. Viral Vectors 101: Pseudotyping

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    ...2006) Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org...

50. Lab to Office Culture Shock

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    ...everyone has a voice and the group can come to a mutual agreement is an amazing thing to be a part of and...