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Showing: 101 - 120 of 425 results
  1. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...10.3389/fimmu.2017.01589 Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation...https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung S, et al (2021) Nanobodies from camelid mice and llamas...
  2. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...180. PubMed PMID: 11815306. 4. Nicole Kresge, R. D. S. and R. L. H. “The Discovery of Avidin by Esmond E... (2004). PubMed PMID: 20353954. 5. Kay, B., Thai, S., & Volgina, V. “High-Throughput Biotinylation of ...PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules...Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu, Y. “A non-radioactive method for ...PMC4439073. 12. Ludwig, L. B., Hughes, B. J., & Schwartz, S. A. “Biotinylated probes in the electrophoretic mobility...
  3. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire...technique to subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson ...Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature...Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development...
  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...When complexed with a full-length gRNA, native Cas9's two catalytic domains, HNH and RuvC, cleave DNA strands... to dramatically alter levels of expression. Cas9’s ability to alternately serve both as a means of modifying...gRNAs An alternative approach is to modulate Cas9’s nuclease activity by modifying the gRNA with which...nucleotides of complementarity to a target site, Cas9’s DNA binding capability remains intact while its nucleolytic...
  5. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...produce the primary recombinant adenoviral stock (rAdV-S). HEK293 cells are transfected with the AdV plasmid...The resultant lysate is your primary low titer rAdV-S, and can be stored at -80C for later use or used immediately...very low titer. Amplification (1-2 weeks) - The rAdV-S is used to infect more HEK293 cells and produce more...8892868. PubMed Central PMCID: PMC190817. 5. Lee, Cody S., et al. "Adenovirus-mediated gene delivery: Potential...
  6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER...Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S, Graham BS, Callewaert N, Schepens B, Saelens X, McLellan...Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation...
  7. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...10.1016/j.jconrel.2019.02.009 Wang D, Mou H, Li S, Li Y, Hough S, Tran K, Li J, Yin H, Anderson DG, Sontheimer...the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes Cas9 from the PGK promoter) and U6 promoter-driven...Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan CA, Rader DJ, Musunuru...
  8. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...technology! Resources and References References Orguc, S., Sands, J., Sahasrabudhe, A., Anikeeva, P., & Chandrakasan...https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S., Loke, G., Fink, Y., & Anikeeva, P. (2019). Flexible...C8CS00710A Sahasrabudhe, A., Rupprecht, L. E., Orguc, S., Khudiyev, T., Tanaka, T., Sands, J., Zhu, W., Tabet...
  9. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher... Scher, D. S. (1994). Isotype choice for chimeric antibodies affects binding properties. In Journal of...
  10. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia virus DNA topoisomerase...coli is sequence specific."  Proc Natl Acad Sci U S A.  1991 Nov 15;88(22):10104-8. PubMed PMID: 1658796...synthesis using vaccinia DNA topoisomerase. Shuman S. J Biol Chem.  1994 Dec 23;269(51):32678-84. PubMed...
  11. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...they think they are.  References Berns KI, Adler S (1972) Separation of two types of adeno-associated...Maynard LH, Smith O, Tilmans NP, Tham E, Hosseinzadeh S, Tan W, Leenay R, May AP, Paulk NK (2019) Fast-Seq... . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer...
  12. The Pleiades Promoter Project

    Type
    Collection
    ...Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J...selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94. doi: 10.1073/pnas...
  13. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM...
  14. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...out, might depend more on the individual sequence(s) than on how well that sequence fits into the five...Knight, T. F., Thomas C. Evans, J., & Lohman, G. J. S. (2018). Comprehensive Profiling of Four Base Overhang...Potapov, V., Bilotti, K., Pokhrel, N., & Lohman, G. J. S. (2022). Rapid 40 kb Genome Construction from 52 Parts.... B., Bilotti, K., Cantor, E. J., & Lohman, G. J. S. (2020). Enabling one-pot Golden Gate assemblies of...
  15. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...26225354. PubMed Central PMCID: PMC4515362 Robinson, W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid...Rous sarcoma virus: Initiation of synthesis with 70 S viral RNA as template. Journal of Molecular Biology.../0022-2836(73)90277-5. PubMed PMID: 4355596 King, S. R., Duggal, N. K., Ndongmo, C. B., Pacut, C., & Telesnitsky...18094172. PubMed Central PMCID: PMC2258930 Hu, W.-S., & Temin, H. M. (1990). Genetic consequences of packaging...
  16. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H., Lim, Z. F. S., & Ng, S. K. (2021). HEK293 cell line as a platform...Plasmids and Resources References Shaw, G., Morse, S., Ararat, M., & Graham, F. L. (2002). Preferential...
  17. CRISPR Antimicrobials

    Type
    Blog Post
    ... et al. used a phagemid system to target virulent S. aureus, a common hospital-borne infection. Two rounds...phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just 24 hours.   ... to target the sensitive population. Yosef et al.’s proof-of-concept work targeted two distinct beta-lactam...antibiotic-resistant bacteria.” Proc Natl Acad Sci U S A. 9;112(23):7267-72. (2015). PubMed: PMID 26060300...
Showing: 101 - 120 of 425 results