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Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

Summary of degron tags along with their applications, advantages, and several commonly used tags.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Learn about evaluating DNA quality with Spectophotometry using a Nanodrop

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Learn about how Cascade-Cas3 can be used as a CRISPR tool to degrade large fragments of DNA.

Magnetoresponsive proteins have seemed like a dream... but Andrew York's lab has developed and deposited one, opening the possibility of magnetoresponsive tools!

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

Marc Zimmer's book, The State of Science, examines broad issues in science including racism and sexism, peer-review, funding, CRISPR, and the politicization of science.

David Liu's lab analyzed how COVID-19 affected their plasmid distribution.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

Learn about aptamer-based fluorphores and how they can be used to brighten up your experiments.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Addgene interviews Hodaka Fujii of Osaka University to learn about his lab's enCHIP system, their new CRISPR tools, community involvement, and more.

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Find materials created at Addgene, including empty backbones, control vectors, viral packaging and expression plasmids, and more.