We narrowed to 277 results for: ALK
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TypeBlog Post...I was in luck when I attended a very interesting talk by Prof. Erin M Schuman, a former Caltech researcher...links the azido containing AHA to a fluorescent alkyne tag. The newly synthesized and tagged proteins ...
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Human Germline Editing Using CRISPR
TypeBlog Post...published August 2nd in Nature gives us even more to talk about, as it represents another step towards CRISPR...comprised of individuals with varied backgrounds. Talking about this study with non-biologist friends Like... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...is slow. Alkaline phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post... Image from Nielson and Voigt (2014). We talked with Voigt about this collision that’s taking place... worked in each case. Sometimes in CRISPR people talk about off-target interactions. That’s relevant if... -
Engaging with science and society at pgEd
TypeBlog Post...organize events that bring different voices together to talk about genetics. In addition to events that we put...interface of science and society, and an ability to talk about science in a language that can be understood... -
10 Basic tips for mammalian cell culture
TypeBlog Post...before you start. Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...model. Until then, you can only wonder what I am talking about. How do researchers use physical models of... over and over in their hands. They then start talking to themselves as they compare the features displayed... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post... our search page (check out this video for a walkthrough of the ways you can search our website). A quicker...about Our New Citations Feature Check out Our Walkthrough of the Deposit Process Learn How We Do Quality... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...research into the classroom. I had the opportunity to talk with Provost recently about his development of what...Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko, J., Keene, A. et al. 2022. Course-based undergraduate... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post... cells). The partition NTPase is recruited and “walks” each plasmid to a separate daughter cell. When ...centromere-like region, and the NTPases end up “walking” the same plasmids to one daughter cell, instead... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...graciously took me for coffee and spent an hour with me talking about the tools I could use, and some experimental...I finally felt like I had a network of people to talk to and attend sessions with.” These sentiments were... -
Hot Plasmids - November 2023
TypeBlog Post...use an orthogonal substrate, alkyne-phenol, to label proteins with an alkyne group for click chemistry reactions... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...then convince the contacts to give me some time to talk or to at least read my emails. Then pitching myself...meet collaborators for lunch, make a point of talking to them at a seminars, or even set up regular meetings... -
Transferable Skills Guide: Career Planning Resources
TypeBlog Post...? Make an effort to meet people. Attend smaller talks within the bigger conference, especially ones that...that are more career-focused. After these talks, introduce yourself to the speaker and offer your business... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...involves, and why you might enjoy doing it. I’ll also talk about the skills you’ll need to do well as a healthcare... career in healthcare consulting, I found that talking to alumni, friends, and friends of friends who ... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...living mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological Studies) Multiple ...light-oxygen-voltage (LOV) domain. They can also be used in crosstalk-free three-color imaging with green and red GFP-like... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...discovered that many of my old suitemates stopped talking to each other if I was not there to arbitrate for... broker a compromise well, you can help everyone walk away from a conflict happy, feeling they each got... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post... different approaches to be familiar with when talking about IF, direct and indirect. For direct IF, researchers... Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy/ Uhlen M, Bandrowski A... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...requirements are similar to standard IF. Here, we’ll walk you through the necessary types of controls for ...., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M., & Weissgerber, T. L. (2021... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...Pancreas AAV6, AAV8 Wang et al., 2006 Retina 7M8 Dalkara et al., 2013 Skeletal muscle MyoAAV Tabebordbar...1359–1368. https://doi.org/10.1089/hum.2008.123 Dalkara, D., Byrne, L. C., Klimczak, R. R., Visel, M., ...