We narrowed to 391 results for: ARIA

... et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP after being targeted...changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus...the test by infecting HEK-293 cells with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for...the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base editing event...

...for synaptic glutamate response. They generated variants optimized for different applications (v82 has ... and powerful high-throughput screen to select variants based on performance metrics specifically for ...BeadScan platform for screening candidate biosensor variants for performance under two-photon fluorescence ...heat with B-gTEMP  Measuring local temperature variations and heat transfer at the subcellular level requires...

...shell packages the vector’s genetic material, and variations between serotypes can change how the viral particle...currently understand it. Tropism depends on many variables, so be sure to check the literature for previous...D. V., & Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient Retrograde Access to Projection...An Introduction to AAV Viral Vectors 101: AAV Variables that Matter Viral Vectors 101: Systemic Capsids...

...response recorded would be due to natural biological variation. This information is critical for correctly interpreting...of the same sample and are included to assess variability in the experimental protocol. In the experiment...results vary considerably, then the high degree of variability in the process could make it difficult to obtain...assess naturally occurring random biological variability. If one were studying the effects of tau kinase...

...any optogenetic experiment. There are numerous variants for the different types of opsins available and...cell-type specificity, the kinetics of the opsin variant, and other particularities that best suit your ...these reviews for a discussion of the different variants [1, 2, 4]. And, of course, the best way to learn...learn about the many different opsin variants and how they can be used is to consult the many primary research...

...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

...Pasteur, Paris) that changed genome editing in the malaria community for the better (Nat. Biotechnol., 2014...Plasmodium falciparum (the parasite that causes malaria). I attribute their success to technical expertise...the software piñata has been filled with slight variations on a theme without many resources to review them...Ghorbal, Mehdi, et al. "Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9...

...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient...

...photoactivatable repressor (dCas9). The activity of all variants was reversible and repeatable. Since these initial...CRISPR–Cas9 activity switching via a novel optogenetic variant of AcrIIA4 (CASANOVA). As shown in the figure below...Publication System nickname Photoactivatable moiety Cas9 variatn CRISPR protein application Split Systems / Light-induced...Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Research 44: . https://doi.org/...

...developing transgenic mice that express camelid variable domains (aka nanomice), which gives researchers...modifications made to generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice... camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586...

... sensor. This sensor was engineered from a GFP variant and a DA receptor such that GFP fluoresces when...when the receptor is bound by DA. Two variants of the sensor, dLight1.1 and dLight1.2, exhibit high (submicromolar...GRABDA sensor based on a similar strategy: a GFP variant bound to a DA receptor, such that the GFP fluoresces...

...Barcoding libraries Just as physical barcodes help librarians track individual books, genetic barcodes allow... to observe clonal dynamics and cell lineage. Variations of this library allow tracking of transcription...libraries have been used to systematically explore variations in the spike protein, teaching us more about ...

...AAV1 Biosensor Marianne Fyhn New viral prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

...Polyclonal antibodies produced in animals may be variable among animals and bleed dates. The development...as other affinity reagents such as single-chain variable fragment (scFvs) and nanobodies. Addgene will ...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080...

... are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...In mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best...measurements. Here, EGFP outperforms the improved folding variants, presumably due to the lower growth temperature...

...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...subclones within a single well reduced well-to-well variability, while the readout was able to be deconvoluted...

...Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting the binding region of...optimizing enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by ... reducing recombination by finding acceptable variations in DR sequences. Figure 2: comparison ...

...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components... other three bases, which limits the level of variation that can be introduced.   Diversification is...

...technology. Nano-lanterns contain a Renilla luciferase variant fused to an FP; when supplied with a luciferase...BRET). Photons emitted by a Renilla luciferase variant are used to excite the fused fluorescent protein...Addgene. Suzuki, Kazushi, et al. “Five colour variants of bright luminescent protein for real-time multicolour...

...created a normalization mechanism to “collapse” variations and synonyms of the same term that appear in ...promoter “Syn”, that, when selected, will match variations present in the data such as synapsin, hsyn synapsin...synapsin, hSynap. Matching We capture multiple variations of the same information in our search indices...