... Having incorporated a discovery-based lab on planarian regeneration into one of my own courses, I was...required for stem and progenitor cell maintenance in planarians in Developmental Biology (Kimball et al., 2020...undergraduates conducting NIH- and NSF-funded research on planarian flatworms both in and out of the classroom.   ...required for stem and progenitor cell maintenance in planarians. Developmental Biology. 457:119-127. Knutson,...

... et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP after being targeted...changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus...the test by infecting HEK-293 cells with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for...the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base editing event...

...vivo. Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous...PE6d are optimized for dual-AAV systems. These variants use different RT enzymes and performed large edits...such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...edit.   Figure 1: Choosing the best PE6 variant for your application. Image reused form Doman and...

...for synaptic glutamate response. They generated variants optimized for different applications (v82 has ... and powerful high-throughput screen to select variants based on performance metrics specifically for ...BeadScan platform for screening candidate biosensor variants for performance under two-photon fluorescence ...heat with B-gTEMP  Measuring local temperature variations and heat transfer at the subcellular level requires...

...response recorded would be due to natural biological variation. This information is critical for correctly interpreting...of the same sample and are included to assess variability in the experimental protocol. In the experiment...results vary considerably, then the high degree of variability in the process could make it difficult to obtain...assess naturally occurring random biological variability. If one were studying the effects of tau kinase...

...6xHis Cre fused to supernegatively charged GFP variant T7 Bacterial Liu 62657 pAYC iCre CAG Mammalian ...9xGGS-Cre-6xHis Cre fused to highly cationic GFP variant T7 Bacterial Liu 62939 pET-Cre-6xHis Cre-6xHis ...

...any optogenetic experiment. There are numerous variants for the different types of opsins available and...cell-type specificity, the kinetics of the opsin variant, and other particularities that best suit your ...these reviews for a discussion of the different variants [1, 2, 4]. And, of course, the best way to learn...learn about the many different opsin variants and how they can be used is to consult the many primary research...

...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

...Pasteur, Paris) that changed genome editing in the malaria community for the better (Nat. Biotechnol., 2014...Plasmodium falciparum (the parasite that causes malaria). I attribute their success to technical expertise...the software piñata has been filled with slight variations on a theme without many resources to review them...Ghorbal, Mehdi, et al. "Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9...

...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient...

...photoactivatable repressor (dCas9). The activity of all variants was reversible and repeatable. Since these initial...CRISPR–Cas9 activity switching via a novel optogenetic variant of AcrIIA4 (CASANOVA). As shown in the figure below...Publication System nickname Photoactivatable moiety Cas9 variatn CRISPR protein application Split Systems / Light-induced...Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Research 44: . https://doi.org/...

... sensor. This sensor was engineered from a GFP variant and a DA receptor such that GFP fluoresces when...when the receptor is bound by DA. Two variants of the sensor, dLight1.1 and dLight1.2, exhibit high (submicromolar...GRABDA sensor based on a similar strategy: a GFP variant bound to a DA receptor, such that the GFP fluoresces...

...developing transgenic mice that express camelid variable domains (aka nanomice), which gives researchers...modifications made to generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice... camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586...

...Polyclonal antibodies produced in animals may be variable among animals and bleed dates. The development...as other affinity reagents such as single-chain variable fragment (scFvs) and nanobodies. Addgene will ...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080...

...AAV1 Biosensor Marianne Fyhn New viral prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

... are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...In mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best...measurements. Here, EGFP outperforms the improved folding variants, presumably due to the lower growth temperature...

...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...subclones within a single well reduced well-to-well variability, while the readout was able to be deconvoluted...

...Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting the binding region of...optimizing enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by ... reducing recombination by finding acceptable variations in DR sequences. Figure 2: comparison ...

...created a normalization mechanism to “collapse” variations and synonyms of the same term that appear in ...promoter “Syn”, that, when selected, will match variations present in the data such as synapsin, hsyn synapsin...synapsin, hSynap. Matching We capture multiple variations of the same information in our search indices...

...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components... other three bases, which limits the level of variation that can be introduced.   Diversification is...