We narrowed to 277 results for: Alk
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TypeBlog Post...is slow. Alkaline phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase...
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Antibodies 101: Conjugation
TypeBlog Post...conjugates are Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Various substrates for each ... are ready to use off the shelf. So why are we talking about this? Even though there are so many commercially... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post...work differently based on their audience. How you talk about your experiments to your PI, your colleagues...difficult to know what kind of plate they were talking about during the meetings. And “plate” certainly... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post... Image from Nielson and Voigt (2014). We talked with Voigt about this collision that’s taking place... worked in each case. Sometimes in CRISPR people talk about off-target interactions. That’s relevant if... -
Engaging with science and society at pgEd
TypeBlog Post...organize events that bring different voices together to talk about genetics. In addition to events that we put...interface of science and society, and an ability to talk about science in a language that can be understood... -
10 Basic tips for mammalian cell culture
TypeBlog Post...before you start. Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...model. Until then, you can only wonder what I am talking about. How do researchers use physical models of... over and over in their hands. They then start talking to themselves as they compare the features displayed... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post... our search page (check out this video for a walkthrough of the ways you can search our website). A quicker...about Our New Citations Feature Check out Our Walkthrough of the Deposit Process Learn How We Do Quality... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...research into the classroom. I had the opportunity to talk with Provost recently about his development of what...Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko, J., Keene, A. et al. 2022. Course-based undergraduate... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post... cells). The partition NTPase is recruited and “walks” each plasmid to a separate daughter cell. When ...centromere-like region, and the NTPases end up “walking” the same plasmids to one daughter cell, instead... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...graciously took me for coffee and spent an hour with me talking about the tools I could use, and some experimental...I finally felt like I had a network of people to talk to and attend sessions with.” These sentiments were... -
Hot Plasmids - November 2023
TypeBlog Post...use an orthogonal substrate, alkyne-phenol, to label proteins with an alkyne group for click chemistry reactions... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...then convince the contacts to give me some time to talk or to at least read my emails. Then pitching myself...meet collaborators for lunch, make a point of talking to them at a seminars, or even set up regular meetings... -
Transferable Skills Guide: Career Planning Resources
TypeBlog Post...? Make an effort to meet people. Attend smaller talks within the bigger conference, especially ones that...that are more career-focused. After these talks, introduce yourself to the speaker and offer your business... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...involves, and why you might enjoy doing it. I’ll also talk about the skills you’ll need to do well as a healthcare... career in healthcare consulting, I found that talking to alumni, friends, and friends of friends who ... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...discovered that many of my old suitemates stopped talking to each other if I was not there to arbitrate for... broker a compromise well, you can help everyone walk away from a conflict happy, feeling they each got... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post... different approaches to be familiar with when talking about IF, direct and indirect. For direct IF, researchers... Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy/ Uhlen M, Bandrowski A... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...living mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological Studies) Multiple ...light-oxygen-voltage (LOV) domain. They can also be used in crosstalk-free three-color imaging with green and red GFP-like... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...requirements are similar to standard IF. Here, we’ll walk you through the necessary types of controls for ...., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M., & Weissgerber, T. L. (2021... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...Pancreas AAV6, AAV8 Wang et al., 2006 Retina 7M8 Dalkara et al., 2013 Skeletal muscle MyoAAV Tabebordbar...1359–1368. https://doi.org/10.1089/hum.2008.123 Dalkara, D., Byrne, L. C., Klimczak, R. R., Visel, M., ...