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...becomes much more complex. “How do I reach out to potential mentors?” “Where do I start with picking universities...applying to a school that requires its students to do rotations in different labs before selecting an advisor...is the right fit. Reaching out to PIs also means doing plenty of mini-interviews; these professors will...universities offer free practice sessions for students doing virtual interviews, so check if yours does and then...things like sickness or scheduling issues can and do interfere with those in-person plans. Virtual options...

...blogging, videos, lectures, exhibits, social media… how do you choose? Which way is the right way?  First of...solid understanding of their audience demographics. Do your research so you can go to where your audience...2005, but XKCD is pretty successful!  What resources do you have?  No matter the project, I can guarantee... multiple points. Before you embark on a project, do your best to understand what kind of resources - ...information to tell a compelling story in an image or do you need a full exhibit? In a five minute activity...already actively interested in scicomm. Difficult to do in-depth storytelling. Requires regular content production...

... an immunology PhD student at Harvard University. Do you have a gene of interest but have run into a wall...seeing a phenotype since additional genes that can do the same function are less likely to exist). If there...abdomens, males have black coloration whereas females do not. Also, males have sex combs on their forelegs...forelegs which are black, bristly hairs. Females do not have sex combs. To make genetic crosses properly, it...chromosomes are miniscule, so researchers generally do not manipulate them. Essentially, the X, second, ...website. One interesting thing to know is that males do not recombine chromosomes during meiosis. This can...

... Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed searching for research...research articles? Well then, my scientist friend, do I have a handy tool for you.   With a few quick ...!). Making the find plasmids badge appear So how do you make this magical Addgene badge appear? First...

...Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should...

...Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should...

...use the Gal4/UAS system, but the available reagents do not match your needs. If YFG is thrillingly new, ...germline. To begin this process, you will first need to do some molecular cloning. Depending on whether you ...transformed bacteria, but you might be wondering, “How do I know which of my flies have the desired integration...Outsourcing the plasmid injections is much easier than doing it yourself. Depending on your strategy and the ...important for adaptive immune responses, which flies do not have!) (4). The power of Drosophila as a model...

...research. Why are sex and gender analysis important? Doing research wrong costs lives and money. According ...drugs fail, they cause human suffering and death. Doing research right has the potential to save lives and...researchers consider the sex of their cells when doing experiments—which can lead to failed research. For...resource for research design is Kalpit Shah et al., “Do you know the sex of your cell?” I can’t tell you ...drug development – costs far greater than those of doing the research correctly from the get go. The future...

...analyses is to keep track of why you are doing what you are doing. Another common challenge is to keep track... a few years ago when my advisor and I decided to do a massive RNA-seq analysis. I had never interacted...time you use them, leave them in their own folder. Doing so will ensure that you are in fact using the same...did something or what the result was, so try not to do this. These are the simple rules that I try to follow...

...wall of bacterial cells; however, Mycoplasma species do not possess cell walls. As a result, Mycoplasma are...treatment aren’t followed closely, you could be doing more harm than good, even with the best of intentions...take-home? Always take your medication as prescribed. How do we know which bacteria are resistant to antibiotics...frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic resistance?  ... to evolve and survive for millennia. What we can do, however, is fight smarter, not harder. We can utilize...resistance can be scary to think about, but we can all do our part to learn all we can, and minimize both our...

...wages, overtime tracking? What are institutions doing? Institutions are now preparing to comply with the...postdocs resource on what institutions are actually doing (using the list of 340 institutions with postdocs...institutions that have not yet announced what they are doing, however, may face greater challenges. Current salary...most of) MIT and the University of Colorado are doing, and are checking with Yale that they are raising...institutions they're thinking about applying to, are doing - for example, your benefits may be changing. Please...of time and a lot of work - but they were able to do so on their own terms. They must feel very relieved...

...presented in this article are those of the author do not represent a formal stance taken by Addgene or... them. Scientific realities limit what policy can do and, in this way, science has always been related... hypotheses mount yet policies and public opinion do not seem to take note. For example, 91% of active...’ ability to uphold their side of the bargain and do the research required to inform the public (8–13)...party while alienating the other While scientists do identify as Democrats more often than Republicans...contact information for your senators all you need to do is select your state. State Legislatures: To find...

...GFDL. Source: Wikipedia, with PyMOL. Author: Bubus Do you have suggestions for other plasmids that should...

...Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should...

...Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should...

...And do remember to breathe!)   Public speaking as a transferable skill The worst thing about doing public...before it, I don’t think I would have had the nerve to do what I did. Until that panicked, split-second decision...people won't notice, or won't remember and those who do are very likely to be sympathetic because public ... notes instead of written speeches. When you’re doing all that, even if it’s exceptionally difficult for...

...causing conformation changes in the AraC dimer. In doing so, the dimer shifts from one that represses transcription...expressing your gene of interest, all you have to do is co-transform pBLADEONLY. That way, a previously... less heterogeneity in the population and you can do all of these more spatially confined experiments ... as those requiring reversibility that you cannot do with AraC,” says Di Ventura. BLADE activates transcription...

...consider. What do academic and non-academic careers in science have in common? You can do quality science... to work/life scheduling Diverse opportunities to do science related jobs that require no bench work Better...available ($)—can outsource a task someone else who can do better, no grant writing Job changes can happen regularly...

...this very blog (you’ll have to let me know how I’m doing a few months down the line!) and to visit scientists...with none of my old friends to fall back on. While doing and presenting undergraduate research at BU, I realized...knowledge. In addition, this position allows me to do another thing I’ve always thoroughly enjoyed: travel...support academia but the skills researchers gain doing PhDs (i.e. technical writing, teaching, presenting...

...they do well and where they need to improve, and they’re also clear about how the person is doing overall...would your plan be? If you could do the thing all over, what would you do differently? Beware of indirect...