We narrowed to 821 results for: EED

...amplified before you conduct your screen. If you need to amplify the library, please refer to the depositor...adequately represented in the population, you’ll need to infect many more cells than the number of plasmids...

... if needed. And since we live in the digital age, make sure to research any software you may need: cost...possible COVID-driven virtual drain. Audience needs high speed internet access. High probability of real-... of any given country have equal access to high-speed internet. Before you begin any work, make sure you...produce an outcome you’re happy with. Know if you’ll need specialized equipment and/or expertise from the ...to tell a compelling story in an image or do you need a full exhibit? In a five minute activity at a street...demanding format. Equipment, expense and expertise needed can increase exponentially with complexity. ... and physically. Easy to incorporate visuals if needed. Traditional media formats are quite competitive...

...just as easily discover you need to add some people if you find there’s a need for an expertise your current... and by offering advice on areas where you might need some help. Today: Teamwork. My first experience ...may be relatively permanent; some teams may only need to exist for a year, or a few months, or even a ...project that never ends is a poorly planned project indeed. Mission team A Mission Team is assembled to make...requires a broad knowledge base is going to necessarily need a diverse group of people to provide that base. ... out the door much faster, but getting helpful feedback from other people who have a stake in the outcome... Social interaction is a good thing Part of you needs to interact with other people, whether you want ...

...additional data needed to rule out alternative models or to strengthen your new model?  PFs need to be regularly...your postdoctoral experience, define what will be needed to achieve your intended next career stage and ...competitive for a Group Leader position, what will you need? Multiple first or corresponding author publications... you get there and more practically, when do you need to be there? A typical biomedical postdoc fellowship...Group Leader position, key first author manuscripts need to be ready to write by Year 3.5. Thus, it is critical... directions of the lab, gives you constructive feedback on your research, and teaches you how to write...mentor is. If the person makes time for you, as needed, this is a strong indicator that this person cares...

...that we chose needed to fulfill certain requirements: Customizable to our specific needs Modern and well-supported...usage of the scripts needs to be customized in the pipeline for the specific needs of the user. Thus, a... repository continues to expand, we realized the need to modernize our approach to handle larger volumes...              DNA sequencing drives our bioinformatics needs       Fig. 1: QC'ing data at Addgene is like...been crucial for us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal... challenge: automating the bioinformatics steps needed to transform the raw sequencing data into a complete...Seqera’s mission and our own, as both address the need to accelerate science using different approaches...

...spent on details that do not yet need to be decided. Figure out what you need to accomplish to get to the ... meeting at all. When no discussion is needed and you just need to give everyone a heads up (for example...everyone’s time by considering the level of meeting you need. Project Meetings: You do not have to include every...workload, updates on current projects, and offer feedback. The employee should ask for input on projects...the manager know if any additional resources are needed. All-Company Meetings: As your organization grows...whole thing together. How long do you actually need? Just like liquids, meetings tend to fill the shape...of their container. Consider whether you actually need a full hour to achieve the purpose of the meeting...

...single most important skill that scientists need to succeed in their work. While it's not always recognized...scientist needs to be able to communicate with their colleagues and peers. Scientists also need to be able...substitute for feedback from others.  Seek out members of your target audience and ask for their feedback before...will have in the world is the value that you can succeed in communicating. Even the most rigorous, insightful... interactions, ask yourself how every piece of feedback you receive generalizes.  If someone suggests ...   To address that 'why' question effectively, you need to make your audience the focus of every sentence... write and step you take.  Why does the audience need to know this? How will they react to it - what will...

...testing new products or methods, using metrics and feedback, taking a look at what works and what doesn’t,...can guide viewers through. I know cameras, but we needed someone else. Design: Jennifer thankfully volunteered.... It’s wonderful and simple to use, but when you need to juggle filming and performing on camera, it’s...No scripting made this a lot easier and I didn’t need to be there directing. Everyone’s schedules were...While this works well for social media videos, we need as much detail as possible when showing lab methods...  Design: The previous cycle made me realize we needed to focus on ways to shoot safely at a distance ...out of reach for Addgene.  So, onto research! I needed to design a system that would allow me to set up...

...that need to be resolved. If there are any forms that need to be signed, or manuscripts that need to be...their ideas confidently while also considering the needs of other people. Some students and postdoctoral .... It is in your supervisor’s interest that you succeed, and if you cannot resolve a problem on your own...reach out for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors...brainstorm about solutions that will meet both of your needs. 6) Put important agreements in writing One of ...meeting with an email that summarizes what you have agreed upon and your action items. This will give your... what you have discussed and add suggestions if needed. 7) Always follow through on your end of the deal...

...kits are guaranteed to yield endotoxin-free DNA. Once you know the type of DNA prep you need, you can ... right for you. What type of plasmid prep do you need? As we covered in a previous post on plasmid preps...downstream application will dictate how much DNA you need to recover and the level of quality required. Some...Figure 1 illustrates the three grades of DNA you may need based on their relative quality and quantity.   ... and up). But perhaps more importantly, the DNA needs to be free of contaminants, such as bacterial endotoxins...as their input (Table 1). When you feel like you need just a bit higher DNA yield, but not enough to go...increasing your input to increase your output. But, exceeding a kit’s capacity can actually reduce your ultimate...

...avoid bleeding as much as possible. However, when you need to label multiple markers, some bleeding is often...cytometry is commonly referred to as bleeding. When two fluorophores bleed into each other, it means that the...different target proteins in your flow analysis, you'll need to use fluorophores that attach to the target. When... When you design a flow experiment, you'll need to pick a fluorophore for each target and ensure the fluorophores...properly label many markers with any fluorophore. Bleeding Before we start building your first panel, we'... a protein and FITC an organic molecule), their bleed is so strong that these spectra almost overlap. ...As eGFP emits fluorescence by itself and does not need to be bound through a separate fluorophore, we have...

...Horticulutre Biotechnology, he did his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan State...award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that will help accelerate...about Dr. Kwapata’s current work and thoughts on the need for more scientific collaboration across the globe...:41 : Kingdom on the difficulties surrouding and need for biotech research in Malawi 11:41-14:53 : Kingdom's...challenges in Malawi, and more 23:45-28:07 : How Seeding Labs is helping advance Kingdom's research 28:08... Kwapata for taking the time to talk to us and Seeding Labs for connecting us with him. Learn more about...about our recent collaboration with Seeding Labs here. Special thanks to our sound engineer/producer, Eddy...

...competitive CV You don't necessarily need a Nature/Cell/Science paper. You do need multiple first/corresponding...solicited for feedback), possibly the Dean, and even some people in your field that still need to understand...members will NOT be in your area. Therefore, documents need to minimize jargon. Equally importantly, you really...me that their search committee is different and needs to be told all of the nuances of the research in...Bottom line is to appreciate that your audience needs to understand your proposal to give you proper consideration...your research because you waded too deep into the weeds. Check out Joanne Kamens's "10 Steps to a Perfect...idea in a swamp of unnecessary details. You DO NOT need to present a detailed review of your field or the...

...Luna, Joan Reede, Alberto Roca, and Jessica Tytell made it was obvious that more data is needed for us to...outcomes. Some of the more specific contours of what we need to know in order to even assess the current state...the professional skills and research independence needed to pursue his or her chosen career path,” but individual...companies and organizations (including Addgene), that need people who are data oriented, scientifically minded...graduate students and postdocs. Trainees however, need to be made more aware of the fact that these positions...highlights just this point. As part of this, trainees need to be able to network and interact more with people...well as young PIs and all gave advice on how to succeed in publishing in the current academic environment...

...hear you saying “We know this already!” But, you do need to be able to research and write your application...fresh eyes and correct it (and, believe me, you will need to correct it). It’s easy for reviewers to spot ...department) to read over your application and give you feedback. They’ve most likely been through the process ... your area are looking for. Remember though, you need to leave them time to read it, give it back to you...Funding agencies want value for their money so you need to make it clear that you will be doing your research...you have been working for the last few years, you need to be very clear that you are staying because it...the world for your research to rapidly progress. Need Help Building Your Scientific Network? Check out...

...-international focus. Candidates in need of an industry H-1B need to be aware of the April 1 deadline ...necessary and no job offer is needed for this category. To qualify, you need to satisfy 3 out of the 10 ... their work in the US have the added burden of needing sponsorship. If you are in this position, you should... green cards that are well-tailored to meet the needs of researchers. The categories are as follows: ...organizations can be a good choice for individuals needing sponsorship. Academic H1B The academic H-1B allows...your case – the invitation to be a peer reviewer needs to be addressed to your own personal email directly... not allow for individuals to self-petition. You need the support of your institute to do this one. Universities...

...: expert No-needle techniques The term “injection” is most often associated with needles, but it may ...surprise you that they don’t always require needles. If needles make you cringe or your experiment will allow...in mice. Image created with biorender.com.   Needle techniques Intravenous Intravenous (IV) injections...actually requires more technical skill to place the needle in the correct vein without collapsing it. Retro-orbital... (IT) injections are probably the most advanced needle injection technique. Intrathecal injections are... allow you to avoid poking the mice, these “no needles” techniques may come in handy. These techniques...technique involves administering medication through a feeding tube with a bulb on the end, which prevents accidental...

...list. Need feedback on their work but prefer to be approached as peers rather than subordinates Need mental... is hard and most people have some learning they need to do before they can be good at it. Like any other...work harder and contribute more. Assuming basic needs are being met (i.e. enough pay and benefits to live...to work mostly at home. Having a high degree of freedom and diversity built into their jobs Chance to do... to identify the work style of each team member) Need challenging work, opportunities to pursue and problems...people, not just scientists) Are self-directed, but need precise leadership and support from their superiors...comfortable with imposed deadlines (there’s that need for flexibility, sometimes taken to an extreme by...

...aliquoted to reduce the number of freeze-thaw cycles needed when using the virus. A small aliquot is used to...hundreds to millions of distinct plasmids, all of which need to be represented in the same proportion after amplification...library can drastically impact what conditions it needs to properly amplify.  Amplification protocols therefore...protocol, or have constructed your own library, you’ll need to develop your own protocol that amplifies without...Verification Once your library has been amplified, you'll need to verify your library by next-generation sequencing...If you’re ordering a pre-made library, you don’t need to worry about amplifying and producing lentivirus...libraries aren’t quite plug-and-play: you’ll still need to titer and optimize before you can transduce your...

...addition to all of the documents you submit, you will need to present a "chalk talk." However, few, if any,...search committee member cannot either. Therefore, you need to be able to describe experimental approaches in... if the aim is successful. Note that aims do not need to be grant aims. Each aim could be the basis of...Once you have developed your research vision, you need to package it as a roughly 30 minute chalk talk....interesting. For example, “there is a significant need for healthy livers for transplants. Growth of new...What will you have accomplished in 5-10 years? You need to be able to describe what success looks like. ...Describe the question, the bare minimum of background needed to understand the problem and goal, the approach...