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Showing: 101 - 120 of 824 results

  1. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...additional data needed to rule out alternative models or to strengthen your new model?  PFs need to be regularly...your postdoctoral experience, define what will be needed to achieve your intended next career stage and ...competitive for a Group Leader position, what will you need? Multiple first or corresponding author publications... you get there and more practically, when do you need to be there? A typical biomedical postdoc fellowship...Group Leader position, key first author manuscripts need to be ready to write by Year 3.5. Thus, it is critical... directions of the lab, gives you constructive feedback on your research, and teaches you how to write...mentor is. If the person makes time for you, as needed, this is a strong indicator that this person cares...

  2. Bioinformatics at Addgene

    Type
    Blog Post
    ...that we chose needed to fulfill certain requirements: Customizable to our specific needs Modern and well-supported...usage of the scripts needs to be customized in the pipeline for the specific needs of the user. Thus, a... repository continues to expand, we realized the need to modernize our approach to handle larger volumes...              DNA sequencing drives our bioinformatics needs       Fig. 1: QC'ing data at Addgene is like...been crucial for us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal... challenge: automating the bioinformatics steps needed to transform the raw sequencing data into a complete...Seqera’s mission and our own, as both address the need to accelerate science using different approaches...

  3. How to Lead a Great Meeting

    Type
    Blog Post
    ...spent on details that do not yet need to be decided. Figure out what you need to accomplish to get to the ... meeting at all. When no discussion is needed and you just need to give everyone a heads up (for example...everyone’s time by considering the level of meeting you need. Project Meetings: You do not have to include every...workload, updates on current projects, and offer feedback. The employee should ask for input on projects...the manager know if any additional resources are needed. All-Company Meetings: As your organization grows...whole thing together. How long do you actually need? Just like liquids, meetings tend to fill the shape...of their container. Consider whether you actually need a full hour to achieve the purpose of the meeting...

  4. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...single most important skill that scientists need to succeed in their work. While it's not always recognized...scientist needs to be able to communicate with their colleagues and peers. Scientists also need to be able...substitute for feedback from others.  Seek out members of your target audience and ask for their feedback before...will have in the world is the value that you can succeed in communicating. Even the most rigorous, insightful... interactions, ask yourself how every piece of feedback you receive generalizes.  If someone suggests ...   To address that 'why' question effectively, you need to make your audience the focus of every sentence... write and step you take.  Why does the audience need to know this? How will they react to it - what will...

  5. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...testing new products or methods, using metrics and feedback, taking a look at what works and what doesn’t,...can guide viewers through. I know cameras, but we needed someone else. Design: Jennifer thankfully volunteered.... It’s wonderful and simple to use, but when you need to juggle filming and performing on camera, it’s...No scripting made this a lot easier and I didn’t need to be there directing. Everyone’s schedules were...While this works well for social media videos, we need as much detail as possible when showing lab methods...  Design: The previous cycle made me realize we needed to focus on ways to shoot safely at a distance ...out of reach for Addgene.  So, onto research! I needed to design a system that would allow me to set up...

  6. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...that need to be resolved. If there are any forms that need to be signed, or manuscripts that need to be...their ideas confidently while also considering the needs of other people. Some students and postdoctoral .... It is in your supervisor’s interest that you succeed, and if you cannot resolve a problem on your own...reach out for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors...brainstorm about solutions that will meet both of your needs. 6) Put important agreements in writing One of ...meeting with an email that summarizes what you have agreed upon and your action items. This will give your... what you have discussed and add suggestions if needed. 7) Always follow through on your end of the deal...

  7. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...kits are guaranteed to yield endotoxin-free DNA. Once you know the type of DNA prep you need, you can ... right for you. What type of plasmid prep do you need? As we covered in a previous post on plasmid preps...downstream application will dictate how much DNA you need to recover and the level of quality required. Some...Figure 1 illustrates the three grades of DNA you may need based on their relative quality and quantity.   ... and up). But perhaps more importantly, the DNA needs to be free of contaminants, such as bacterial endotoxins...as their input (Table 1). When you feel like you need just a bit higher DNA yield, but not enough to go...increasing your input to increase your output. But, exceeding a kit’s capacity can actually reduce your ultimate...

  8. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...avoid bleeding as much as possible. However, when you need to label multiple markers, some bleeding is often...cytometry is commonly referred to as bleeding. When two fluorophores bleed into each other, it means that the...different target proteins in your flow analysis, you'll need to use fluorophores that attach to the target. When... When you design a flow experiment, you'll need to pick a fluorophore for each target and ensure the fluorophores...properly label many markers with any fluorophore. Bleeding Before we start building your first panel, we'... a protein and FITC an organic molecule), their bleed is so strong that these spectra almost overlap. ...As eGFP emits fluorescence by itself and does not need to be bound through a separate fluorophore, we have...

  9. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...Horticulutre Biotechnology, he did his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan State...award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that will help accelerate...about Dr. Kwapata’s current work and thoughts on the need for more scientific collaboration across the globe...:41 : Kingdom on the difficulties surrouding and need for biotech research in Malawi 11:41-14:53 : Kingdom's...challenges in Malawi, and more 23:45-28:07 : How Seeding Labs is helping advance Kingdom's research 28:08... Kwapata for taking the time to talk to us and Seeding Labs for connecting us with him. Learn more about...about our recent collaboration with Seeding Labs here. Special thanks to our sound engineer/producer, Eddy...

  10. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...competitive CV You don't necessarily need a Nature/Cell/Science paper. You do need multiple first/corresponding...solicited for feedback), possibly the Dean, and even some people in your field that still need to understand...members will NOT be in your area. Therefore, documents need to minimize jargon. Equally importantly, you really...me that their search committee is different and needs to be told all of the nuances of the research in...Bottom line is to appreciate that your audience needs to understand your proposal to give you proper consideration...your research because you waded too deep into the weeds. Check out Joanne Kamens's "10 Steps to a Perfect...idea in a swamp of unnecessary details. You DO NOT need to present a detailed review of your field or the...

  11. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...Luna, Joan Reede, Alberto Roca, and Jessica Tytell made it was obvious that more data is needed for us to...outcomes. Some of the more specific contours of what we need to know in order to even assess the current state...the professional skills and research independence needed to pursue his or her chosen career path,” but individual...companies and organizations (including Addgene), that need people who are data oriented, scientifically minded...graduate students and postdocs. Trainees however, need to be made more aware of the fact that these positions...highlights just this point. As part of this, trainees need to be able to network and interact more with people...well as young PIs and all gave advice on how to succeed in publishing in the current academic environment...

  12. 6 Tips for Grant Writing

    Type
    Blog Post
    ...hear you saying “We know this already!” But, you do need to be able to research and write your application...fresh eyes and correct it (and, believe me, you will need to correct it). It’s easy for reviewers to spot ...department) to read over your application and give you feedback. They’ve most likely been through the process ... your area are looking for. Remember though, you need to leave them time to read it, give it back to you...Funding agencies want value for their money so you need to make it clear that you will be doing your research...you have been working for the last few years, you need to be very clear that you are staying because it...the world for your research to rapidly progress. Need Help Building Your Scientific Network? Check out...

  13. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...-international focus. Candidates in need of an industry H-1B need to be aware of the April 1 deadline ...necessary and no job offer is needed for this category. To qualify, you need to satisfy 3 out of the 10 ... their work in the US have the added burden of needing sponsorship. If you are in this position, you should... green cards that are well-tailored to meet the needs of researchers. The categories are as follows: ...organizations can be a good choice for individuals needing sponsorship. Academic H1B The academic H-1B allows...your case – the invitation to be a peer reviewer needs to be addressed to your own personal email directly... not allow for individuals to self-petition. You need the support of your institute to do this one. Universities...

  14. Common Injection Routes in Mice

    Type
    Blog Post
    ...: expert No-needle techniques The term “injection” is most often associated with needles, but it may ...surprise you that they don’t always require needles. If needles make you cringe or your experiment will allow...in mice. Image created with biorender.com.   Needle techniques Intravenous Intravenous (IV) injections...actually requires more technical skill to place the needle in the correct vein without collapsing it. Retro-orbital... (IT) injections are probably the most advanced needle injection technique. Intrathecal injections are... allow you to avoid poking the mice, these “no needles” techniques may come in handy. These techniques...technique involves administering medication through a feeding tube with a bulb on the end, which prevents accidental...

  15. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...list. Need feedback on their work but prefer to be approached as peers rather than subordinates Need mental... is hard and most people have some learning they need to do before they can be good at it. Like any other...work harder and contribute more. Assuming basic needs are being met (i.e. enough pay and benefits to live...to work mostly at home. Having a high degree of freedom and diversity built into their jobs Chance to do... to identify the work style of each team member) Need challenging work, opportunities to pursue and problems...people, not just scientists) Are self-directed, but need precise leadership and support from their superiors...comfortable with imposed deadlines (there’s that need for flexibility, sometimes taken to an extreme by...

  16. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...aliquoted to reduce the number of freeze-thaw cycles needed when using the virus. A small aliquot is used to...hundreds to millions of distinct plasmids, all of which need to be represented in the same proportion after amplification...library can drastically impact what conditions it needs to properly amplify.  Amplification protocols therefore...protocol, or have constructed your own library, you’ll need to develop your own protocol that amplifies without...Verification Once your library has been amplified, you'll need to verify your library by next-generation sequencing...If you’re ordering a pre-made library, you don’t need to worry about amplifying and producing lentivirus...libraries aren’t quite plug-and-play: you’ll still need to titer and optimize before you can transduce your...

  17. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...addition to all of the documents you submit, you will need to present a "chalk talk." However, few, if any,...search committee member cannot either. Therefore, you need to be able to describe experimental approaches in... if the aim is successful. Note that aims do not need to be grant aims. Each aim could be the basis of...Once you have developed your research vision, you need to package it as a roughly 30 minute chalk talk....interesting. For example, “there is a significant need for healthy livers for transplants. Growth of new...What will you have accomplished in 5-10 years? You need to be able to describe what success looks like. ...Describe the question, the bare minimum of background needed to understand the problem and goal, the approach...

  18. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ... degrading them. You’ll also need to know the wavelength of laser needed to excite them - and it’s a good...experiment - there are a number of outside factors that need to be considered as well. In this post, we’ll touch...antibody. This information is important, and you’ll need to track it throughout your experiments.  Now that...and biological structures, some of which you may need in your experiment.  If you are planning to conjugate...light sensitive, so antibodies conjugated to them need to be stored either in a dark, opaque vial or wrapped...experiment is easy, but what about those times you need more than one?   Multiplex assays Multiplex assays...each with a different signaling molecule, that all need to be detected in the same assay. This is one of...

  19. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...valuable feedback.  Listen. Think about the questions you’ve received, read the comments, seek feedback from...amount of mental thought and effort your audience needs to understand what you’re communicating. While I...striving to obtain?” If the answer is yes, you will need to spell out that connection for your audience. ...blank spaces or to gloss over some aspects when needed. The trick is to communicate your key points well...students know.   Ask the learners themselves for feedback in a variety of ways, like having them restate...about how I was presenting the information. Maybe I needed to remove a distraction, such as pausing or turning...something was intuitive that actually wasn't. Maybe I needed to employ a visual aid instead of describing something...

  20. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...experimental system used, so you’ll need to know what expression pattern you need, and the factors in your system...cells you’re targeting; (3) how much expression is needed to read your experimental output; and (4) the consequences... translate to in vivo efficacy. You’ll therefore need to test potential AAV vectors in your specific experimental... AAV2 (Watakabe et al., 2015) (Figure 1). You’ll need to consider if such properties could help, or harm...affected by the cell and/or tissue type, so you’ll need to test for this in your system if you’re measuring...some neurons can transiently express TH - so you’ll need to check for this in your system and then test each...doxycycline (Favre et al., 2002). They are useful when you need to control the timing of expression in your experiment...
Showing: 101 - 120 of 824 results