We narrowed to 160 results for: Jun;

1. Adeno-associated virus (AAV) Plasmids

   Type

   Collection

   ...which determines the AAV serotype) are used in conjunction with the plasmid that encodes the viral genome...

2. Transferable Skills Guide: Cross-team Communication

   Type

   Blog Post

   ...collaborative lab work. Corralling context and junking jargon I suspected I knew at least one source of...

3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   ...fusions to connexin localized correctly to gap junctions (Shaner et al, 2008). Obligate dimers (such as...

4. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   ... 6.2 (2002): 272. PubMed PMID: 12349826. Xiong, Wenjun, and Constance Cepko. "Distinct expression patterns...

5. Addgene: The First Twenty Years

   Type

   Blog Post

   ...depositors whose plasmids we were entrusted with. So in June 2016, we launched the Blue Flame Award, which recognizes...

6. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   ...predecessor. Important features may include cloning junctions (where most sequence assembly errors occur), deletions...

7. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   ...Shaping the Future of Research: a perspective from junior scientists." F1000Research 3 (2014). Pubmed PMID...

8. Plasmids 101: Cre-lox

   Type

   Blog Post

   ...alternative to Cre-lox, and has also been used in conjunction with it, allowing for two separate recombination...

9. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   ... vectors Behavioral testing is often done in conjunction with a genetic manipulation so that behavior ...

10. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    ...Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule FRET for understanding biomolecular...

11. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    ...CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3...

12. Hot Plasmids February 2024

    Type

    Blog Post

    ...cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem by optimizing the...

13. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    ...Shaping the Future of Research: a perspective from junior scientists."F1000Research 3 (2014). Pubmed PMID...

14. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    ...improving my skills and sharing with other more junior leaders what I had experienced and learnt. Opening...

15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    ... S., Min, S., Bae, J. Y., Choi, J. W., Park, J., Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity...

16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    ...clones displayed proper insertion at the 5’ and 3’ junctions, respectively. These results indicate that PITCh...

17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    ...while a catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control...

18. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    ...pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-K (2018) Global analysis of ...

19. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    ...has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused an estimated total ...

20. MXS Chaining

    Type

    Blog Post

    ...acids. The Journal of biological chemistry. 1962. June;237:1961–1967. Pubmed PMID: 13918810. ...