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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 492 results for: LARGE;

Showing: 101 - 120 of 492 results

  2. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start, the average...different than what was originally planned, due in large part to the feedback we received from our network...

  3. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...optimized by testing various linker configurations in a large-scale functional screen in bacteria. Sensor variants...quite a robust way to make a FRET sensor with a large dynamic range,” he says. Merkx recently applied ...

  4. Worm Expression Resources

    Type
    Collection
    ...fluorescent proteins, and Cre expression plasmids. Large collections or kits are described below and not ...

  5. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Welker Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

  6. Promega Plasmid Collection

    Type
    Collection
    ...structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa) subunit and a small complementary...

  7. CRISPR Guide Update

    Type
    Blog Post
    ...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

  8. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in optimizing...required per system. Applications of these systems The large dynamic range of these systems ensures that they...

  9. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...thing to consider is the size of the conference. Large conferences have many opportunities but can be overwhelming... overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups ...national recognition and the more people you know, the larger your profile in the community Job hunting outside...

  10. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...Musunuru: We have been using the kit to target a large number of genes involved in cardiovascular and metabolic...power here is that you could potentially sequence a large number of individuals and find several mutations...

  11. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...sequence, or if you want to amplify sequences from a large genome organisms like mouse or human. There are ...difficulties in amplifying a template, particularly from a large genome, you can recheck your primer design or optimize...

  15. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...site in the genome you are trying to target play a large role in Cas9 activity and efficiency (Sander and... structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They continued to...

  16. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...

  17. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...previously-reported technique for precisely integrating large DNA sequences into a genome (Anzalone et al., 2022...deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nature Biotechnology... (2024). Efficient site-specific integration of large genes in mammalian cells via continuously evolved...

  18. Finding Your Science Policy Path

    Type
    Blog Post
    ...new way through the integration of science with a large set of other societal pressures including economic...p” policy is non-governmental work happening at large organizations who have to develop policies for their...policy to combat sexual harassment in academia or a large company who decides how they are going pursue regulatory...what better place to do that than at the nation’s largest integrated healthcare system? Defining your why...

  19. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...this latest work, the team designed and screened a large library of iGluSnFR3 mutants, identifying the two... making it ideal for video recordings of large populations of synapses and deep-brain fiber photometry...edits — single-base changes, small deletions, and large insertions up to 12 kb — with efficiencies between...somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making...

  20. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...input to an RSM drives expression of a distinct large serine recombinase that recognizes its own attB ...high copy, ColE1 origin of replication), contains large serine recombinase genes downstream of inducible...faster or more efficiently than others. Multiple large serine recombinases have been described in the literature...
Showing: 101 - 120 of 492 results