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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 286 results for: LUM

Showing: 101 - 120 of 286 results

  1. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from degrading them...

  2. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...calcium dynamics in the cytosol and sarcoplasmic reticulum are of interest to muscle biologists, while neuroscientists... Overview of AAV Encoded Calcium Biosensors Illuminating Epigenetics with a FRET Based Biosensor Fluorescent...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    ...either through a chromogenic or chemiluminescent reaction. Chemiluminescence, which is light produced by ...

  6. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...management software aren’t just limited to large volume facilities; it can be useful in academic labs where...collision. It may even be worth it to have extra columns in your database to the effect of `dev_name` and...

  7. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...reprobing works best with western blots that use chemiluminescent or fluorescent detection, like horseradish...remains, whether that involves adding fresh chemiluminescent substrate or imaging your fluorescent probe...

  8. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...

  9. Of Myc and Men

    Type
    Blog Post
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016...

  13. Plasmids 101: Protein tags

    Type
    Blog Post
    ...metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, a His tag can be fused...with the cleavage reaction taking place between column runs. The TEV protease itself, with various mutations...

  14. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...dark areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel...References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability...

  15. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ... through this same initial QC process using an Illumina MiSeq, but that’s not the only technology Addgene...  Bioinformatics at Addgene After a run on our Illumina MiSeq, the data is imported into our assembly ...

  16. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment...

  17. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp., Bordetella spp...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

  19. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093...CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093...
Showing: 101 - 120 of 286 results