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  3. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...management software aren’t just limited to large volume facilities; it can be useful in academic labs where...collision. It may even be worth it to have extra columns in your database to the effect of `dev_name` and...

  4. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...

  5. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...reprobing works best with western blots that use chemiluminescent or fluorescent detection, like horseradish...remains, whether that involves adding fresh chemiluminescent substrate or imaging your fluorescent probe...

  6. Of Myc and Men

    Type
    Blog Post
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    ...metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, a His tag can be fused...with the cleavage reaction taking place between column runs. The TEV protease itself, with various mutations...

  11. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment...

  12. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp., Bordetella spp...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

  13. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...dark areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel...References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability...

  15. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...sensitivity, and photostability under two-photon illumination than previous GEVIs. Some constructs are also...00916-3. doi: 10.1016/j.cell.2022.07.013 pYtags illuminate RTK signaling Receptor tyrosine kinases (RTKs...

  16. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093...CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... sequencing, (which differ from the short-read Illumina MiSeq sequencing that Addgene uses.) However, ...article.   Long-read sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid sequencing ...
Showing: 101 - 120 of 280 results