We narrowed to 178 results for: Lta;

1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...Shi L, Cheng L, Huang P, Yang H (2018) In vivo simultaneous transcriptional activation of multiple genes...

2. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   ...phenotype. Again ABE8.20-m was the winner. It could simultaneously edit 3 genes (98.1%, 98.3%, or 98.6% efficiency...

3. Hot Plasmids and Viral Preps - March 2021

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   ...system from Jiazhang Lian’s lab that enables simultaneous gene activation, repression, and editing with...

4. Adenoviral Vector Production and Troubleshooting

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   ...and lysed by multiple freeze-thaw cycles. The resultant lysate is your primary low titer rAdV-S, and can...

5. Plasmids 101: FLEx Vectors

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   ...elements to turn off expression of one gene, while simultaneously turning on the expression of another! What...

6. The Materials Science of Optogenetics Experiments

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   ... which can control output of several lasers simultaneously, can be used to set the laser output without...

7. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   ...multiplexing and high resolution allowed the Xu Lab to simultaneously characterize the ‘social networks’ of 157 super...

8. Polymerase Chain Reaction Overview and Applications

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   ... or pathogen identification. Scientists can simultaneously detect several different pathogens in one specimen...

9. Plasmids 101: 5 factors to help you choose the right cloning method

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   ...Similarly, Golden Gate assembly allows one to simultaneously complete an ordered set of scarless ligations...

10. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening ...

11. Plasmids 101: Degron Tags

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    ...which target E3 ligases to specific proteins by simultaneously binding both their target protein and an E3...

12. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    ...accuracy. Golden Gate assembly involves the simultaneous activities of a Type IIS restriction enzyme ...

13. Addgene's Cancer Collection Pages Connect the Research Community

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    ...cell to undergo programmed cell death while simultaneously stimulating growth and division. The combined...

14. Plasmids 101: Multicistronic Vectors

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    ...gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from ...

15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...people to, for example, knock out a gene with simultaneous complementation, with a knockout and expression...

16. Which Fluorescent Protein Should I Use?

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    ...pHTomato (pH sensors), HyPer (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr (glutamate sensor). More ...

17. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ...forward-engineering the central dogma across many genes simultaneously. These parts have been carefully engineered...

18. CRISPR 101: RNA Editing with Cas13

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    ... shrink the construct. They identified ADAR2DD(delta984-1090), which decreases the REPAIRv1 construct ...

19. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...mentioned above, organic solvents can be used to simultaneously fix and permeabilize a sample. But you can ...

20. Droplet Digital PCR for AAV Quantitation

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    ...measure the concentration of multiple targets simultaneously. For AAV titration, you should only need to...