...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...

...buffer. Fill the chamber with 1X MOPS running buffer. Make sure that the chamber is sealed. Load 5 µL of the...Pro-Tip Leaving clear lanes between samples will make quantifying the gel easier. Cover the electrophoresis...

...bottle up/down vigorously for 30 sec (it’s okay to make bubbles). Incubate at RT for 15 min. Note that longer...add the OptiMEM + DNA + PEI solution to the CS5. Make sure that all five layers are covered with media...

.... See below for options if the recombinant band makes up a significant proportion of the DNA pool. Last... weight of each pellet should be ~1-2 g. Pro-Tip Make sure to weigh the empty tube beforehand! If you’...

...the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace is composed ...known mutants at $7.00 a strain. Makerspaces like ours can also make accounts with  Addgene. We now have...of the aspects of this project that makes it ideal for makerspaces is the data is freely available, and...  Funding Makerspaces One of the main challenges when starting a new project at a makerspace is finding...following post was contributed by Derek Jacoby from Makerspace Victoria, CA Over the decades science has become...manifested itself in the creation of Hackerspaces and Makerspaces back in 2007, which function as centres for peer...lectures. There are currently around 1,000 active makerspaces around the world. Hackerspaces.org maintains ...

...experience and make sure your next team works better. In fact, don’t wait to be invited onto a team. Make one yourself...Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d spent my senior year ... For example, when it came time to update how we make and distribute kits, I helped assemble a crack team...Is the goal of your project to launch a product? Make an informed decision about something? Fix a problem...indeed. Mission team A Mission Team is assembled to make progress towards open-ended goals whose metrics ...Team, a like-minded group of co-workers who want to make our organization as environmentally friendly as ...There’s also no end-game here, unless we manage to make Addgene the most environmentally friendly organization...

...they don’t make in wages, they make up for in training. Unfortunately, these low wages also make it so it... trainees pay may also make them more attuned to the bang for their buck - make them demand more services...answered about the makeup and welfare of the many researchers out there who are making great progress on...academic research community to develop a plan to make postdoctoral pay/benefits commensurate with work...how many post-docs there are in the U.S., their makeup (in terms of nationality, gender, race, etc), or... One of the Saturday Hack Day projects sought to make an interactive game that highlights just this point...will help you in your academic publishing goals and make you a better communicator overall. Don’t Write...

...reasoning out the window when making decisions. We think scientists make choices based on logic and reason...’s actually something you CAN choose and it will make a huge difference for your future career and life...Which route should I take to get to work?”; we often make important life decisions without much logic - even...your own style of work and communication before making this important decision. I’ll highlight some of...matter to me?”  and hone in on the factors that will make the most difference for your success. Gather data...of written questions for the PI and lab members.  Make coffee or beer dates to talk to as many lab alumni...energy level in the lab? Does a frenetic atmosphere make you edgy or energized?   Are you an only child and...

...online). Mentee: Make a list of topics/skills you are interested in exploring. Mentor:  Make a list of topics...your career development as your mentor. How do you make the most of this new relationship? Consider adding...take this past the chatting stage you will rarely make any change in your skill sets or activate the helpful...that need work or growth. Here are some ideas for making your meetings productive: Discuss logistics if...communicate between meetings (email, phone, etc.)? Make agendas for your meetings, take informal minutes...goals with a mentor: The secret of accountability To make a mentoring relationship really productive, the ...many mentoring meetings to hone the goals and to make them “SMART.” SMART goals are Specific, Measurable...

...Read this Catalyst Report “Making Mentoring Work” to learn about what makes a good mentoring program. ...guidance. Second, I will offer some advice on how to “make the ask” once you have found someone you admire ...professional presence, approach them for advice on what makes them that way. Is there a senior person whose career...Choose 1-2 tactics to try. One of my favorites is making the pledge to have lunch or coffee with someone...able to: Help identify the skills you will need to make a change Provide perspective on core values at another...mentoring program, consider tapping into it and helping make it work by being an educated mentee. Other opportunities...and time. Consider this option but be cautious and make sure to choose one that comes highly recommended...

...BioBrick parts to make more materials: to make more fuel, to make more chemicals, to make more medicine. ... of DNA, transcription of DNA to make RNA, translation of RNA to make proteins. That's central to everything...systems, what do you want to make? So, tell us readers, what do you want to make?   References: Jerome Bonnet...BioBricks Foundation is seeking to accomplish is to make the engineering of biology much easier. So you could...much fun and so incredibly useful that we wanted to make them free to use by everybody. (For more on these...really plugging into the "stuff sector" if you will, making chemicals and materials, and we anticipate plugging...imagination. The question is: what can you use biology to make? If you think about the BIOFAB collection and really...

...let’s chat about giving science talks and how to make them serve you well. The happy byproduct might just...results in the order they were performed does not make for a memorable presentation anywhere but in a lab...knowing your audience is that it can help you avoid making mistakes. I once made a joke about social science... story. Using stories is one of the best ways to make you and your presentations engaging and memorable...you are more likely to remember the point.   What makes a story? A story is a narrative connecting cause...all the time, you just need to figure out how to make it suspenseful. 4. Start preparing your talk early...them. Of course, if some of your work does relate, make sure to include that material, but don’t force it...

...off your work at a conference, you need to first make your poster. While a poster generally contains the...participants can identify you. You may also wish to make creative use of a footer in your poster. This is...sections, so you can include a lot more. Font size: Making your poster readable While we are talking about...This strategy helps your sections stand out, and makes the entire poster more navigable.  Choose your content...that it will display your data most prominently, making it the star of your presentation. Portrait. This...potentially more catchy, but it can be trickier to make figures fit nicely into circular shapes rather than...color you select for the background, you’ll want to make sure that the color of your font is compatible. ...

...volunteer to lead a charitable cause.  While I can’t make it “all better” for you, I can offer some tips and...that end, here are my personal 10 commandments for making it all work – most of the time. #1 Thou shalt find... so will your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead...management tools and tactics to plan your work and make this happen. Be realistic and: Your co-workers ...done your way. Be patient with yourself; you will make progress over time. #6 Thou shalt throw money at...and deliver your groceries. Do anything you can to make your life easier and to have more time to relax ...enthusiastically foster independence Your partner can make his or her own dinner if you are going away to a...

...doi.org/10.1038/nrmicro3569 Makarova, K. S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S.,...Moya-Beltrán, A., Makarova, K. S., Acuña, L. G., Wolf, Y. I., Covarrubias, P. C., Shmakov, S. A., Silva, ...type III, and type IV. Type I Type I CRISPR systems make up the majority of Class 1, and are the most common..., F. E., Moeller, L., Kocalar, S., Oshiro, R., Makarova, K. S., Macrae, R. K., Koonin, E. V., & Zhang,...doi.org/10.1126/science.adi1910 Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin, E. V. (2014...doi.org/10.1134/s0006297921100114 Koonin, E. V., & Makarova, K. S. (2019). Origins and evolution of CRISPR-Cas.../doi.org/10.1098/rstb.2018.0087 Koonin, E. V., Makarova, K. S., & Zhang, F. (2017). Diversity, classification...

...community: Diversity makes the difference  Association for Psychological Science: Diversity Makes Better Science...formally, governments are the organizations that make and enforce the rules of the lands we live on. Rules...cannot declare that gravity change direction and make things fly up when we drop them. Scientific realities...to an earlier analogy, no amount of shouting will make an apple fall up. As scientist and politician Rush...will fall up instead of down when dropped will not make it happen. In a time when a top official can be ... the job of having evidence”, scientists want to make sure having evidence is the job of everyone in government...Immigration Pollution Healthcare Science education To make scientists relatable (17, 20). This is an effort...

... information makes your manuscript more valuable to the research community. It also makes it more likely...Materials and methods section of their manuscript to make resources easier to identify, find back, and share...enough information to replicate your experiments, making research more reproducible and your results more...reviewers and other researchers to access the data and make up their own minds on whether the data analysis ...reliability of science publications. Peer reviewing To make sure your manuscript is perceived as a reliable ...ts when publishing a scientific manuscript Dos: Make sure your paper fits the scope of the journal you...Guidelines of the journal you are submitting to and make sure your manuscript fits the house style and prerequisites...

...organizations have been working to provide tools that make it easier to share in-depth information about reagents...FPbase are working hard to enable researchers by making it easier to find information about research tools... more descriptive and interactive protocols that make it much easier to learn new experimental techniques...Electronic lab notebooks also play a role here by making it easier to retain searchable, thorough notes ...techniques, an important component of reproducibility is making sure that others can understand your analyses and...data analysis while other organizations like Dryad make it easier for researchers to share data thereby ...published data if you need to. Similarly, FigShare also makes it easier to share data through their curation of...

... to produce constructs and edit genomes so as to make plants, and agricultural crops in particular, resistant...general. We are funded by a charity and since we are making all the tools for plant research here and validating...like for people to use them. The MoClo Plant Kit makes it really easy to clone genes for expression in ... the time. Now there are many, so if you want to make a 10-gene construct, you don’t have to worry about...plants, including many things people already use. It makes the switch to Golden Gate Cloning easier because...pathogens. It’s also easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene... that you need from the kit and getting started making your own constructs with Golden Gate MoClo. The...

...Did you see Blugene’s make-over and our new homepage? If you've visited Addgene’s homepage recently, ...messaging, as well as simplifying the page, and making it all look clean and easy to use.  Easily search...global navigation and menus over the past year, making it much easier to get around our site.  Community...navigation, we also wanted to emphasize the values that make Addgene unique: community, sharing, and fun. The...reagents and knowledge with one another, and Addgene make this process easy and fun every day.  With these...three values with each element. We also set out to make sure the page looked clean, professional, and modern...exploration throughout the site.  Blugene gets a make-over You may also have noticed another big update...