We narrowed to 143 results for: Mycs
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TypeBlog Post...the human gut bacteria B. thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. However, many non-model...
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Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...Global analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...Song, Neng, et al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively ... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post... a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image adapted from Katti ... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...check out these two references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both fine research organisms) - ... -
Hot Plasmids Spring 2024
TypeBlog Post... vectors for CROPseq-multi using selection by Puromycin, Zeocin®, or nuclear expression of BFP2. ... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...Sadowski. 1985. “The FLP Recombinase of the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae. Paques F and Haber JE. Microbiology... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance.... -
Plasmids 101: Repressible Promoters
TypeBlog Post...genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...protein‐tracking studies in the budding yeast Saccharomyces cerevisiae." Yeast 25.9 (2008): 651-659. PubMed... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...Toolkit for Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12,... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...In addition to using a new UNG ortholog from Mycobacterium smegmatis, termed UdgX, they found three proteins... -
Lentiviral Prep Service
TypeCollection... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...human genes along with 500 non-targeting controls Puromycin Doench , Root Mouse knockout pooled libraries ... genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can...Purpose PI 17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV... -
Viral Production
TypeCollection...Control Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination... tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination...contamination using mycoplasma detection kits. The cell line is maintained for ~20 passages before being... -
mTOR Pathway
TypeCollection...Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling...threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40 Also known as AKT1S1...associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53 PDK1 Pyruvate dehydrogenase...10.1016/j.cell.2012.03.017. PubMed PMID: 22500797 . Rapamycin: one drug, many effects. Li J, Kim SG, Blenis ... -
Kazuhiro Oka Lentiviral Vectors
TypeCollection...YET Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre...pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato...-copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro...pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV...