We narrowed to 142 results for: Mycs;
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  TypeBlog Post...bacteria where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM...
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  Mobile-CRISPRi: Bringing CRISPRi to Diverse BacteriaTypeBlog Post...the human gut bacteria B. thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. However, many non-model...
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  Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions EasyTypeBlog Post...Global analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org...
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  Droplet Digital PCR for AAV QuantitationTypeBlog Post...Song, Neng, et al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital...
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  Tips for Screening with Yeast Two Hybrid SystemsTypeBlog Post...Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively ...
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  Tips from the Repository Trenches: Using Barcodes to Track SamplesTypeBlog Post...majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves...
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  Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s ViewTypeBlog Post...Sadowski. 1985. “The FLP Recombinase of the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently...
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  Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A ReceptorTypeBlog Post...check out these two references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive...
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  What's Your Organism? Expanding Genomic Tools via the NSF EDGE ProgramTypeBlog Post...So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both fine research organisms) - ...
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  Four Base Editing Reporters to Monitor and Enrich Editing in Real-timeTypeBlog Post... a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image adapted from Katti ...
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  Hot Plasmids Spring 2024TypeBlog Post... vectors for CROPseq-multi using selection by Puromycin, Zeocin®, or nuclear expression of BFP2. ...
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  CRISPR 101: Homology Directed RepairTypeBlog Post...Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae. Paques F and Haber JE. Microbiology...
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  Multiple Plasmids at a Low Price: Kits from AddgeneTypeBlog Post...et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance....
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  Plasmids 101: Repressible PromotersTypeBlog Post...genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197...
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  Fluorescent Proteins 101: Photoactivatable Fluorescent ProteinsTypeBlog Post...protein‐tracking studies in the budding yeast Saccharomyces cerevisiae." Yeast 25.9 (2008): 651-659. PubMed...
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  Plasmids 101: Modular Cloning Applications and KitsTypeBlog Post...Toolkit for Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12,...
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  CRISPR 101: Cytosine Transversion EditorsTypeBlog Post...In addition to using a new UNG ortholog from Mycobacterium smegmatis, termed UdgX, they found three proteins...
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  Lentivirus PlasmidsTypeCollection...shRNA empty plasmid with puromycin selection. See plasmid 24150 for hygromycin selection. Bob Weinberg ...expression of shRNA with puromycin selection. See plasmid 21916 for neomycin selection. Dmitri Wiederschain...expression of shRNA with neomycin selection. See plasmid 21915 for puromycin selection. Dmitri Wiederschain...19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion with puromycin resistance. Can be used for cDNA expression. David...pLenti-puro 3rd CMV-driven expression of cDNA with puromycin selection. Ie-Ming Shih 25737 pSLIK-Hygro 3rd ...expression, gateway plasmid, and constitutive hygromycin selection. See Shin et al. (2006) for additional...Inducible lentiviral expression, TRE-gateway, with puromycin selection. See Root Lab pLEX and pLIX Gateway ...
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  Lentiviral Prep ServiceTypeCollection... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 (Discontinued) Human CRISPR...human genes along with 500 non-targeting controls Puromycin Doench , Root Mouse knockout pooled libraries ... genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can...Purpose PI 17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV...
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  Viral ProductionTypeCollection...Control Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination... tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination...contamination using mycoplasma detection kits. The cell line is maintained for ~20 passages before being...