We narrowed to 417 results for: NUC

...to an oligonucleotide depending on your downstream application. Biotin labelled oligonucleotides can be...that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin can be added to either terminus... becomes reactive and forms a link with nearby nucleic acids (McInnes et al., 1990). Photobiotinylation...specific dsDNA, ssDNA or RNA–protein interactions.” Nucleic Acids Research, 23.18 (1995): 3792–3793. PubMed...

...the gRNA (composed of a ~20 nucleotide targeting sequence and ~75 nucleotide scaffold sequence) is consistent...commonly tagged with a nuclear localization sequence to enhance targeting to the nucleus, and several codon...plant species or cell type (Belhaj et al., 2013). Nuclease dead Cas9 (dCas9) based activators (such as dCas9...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan...

...various MOIs. The presence of the 428 bp Surveyor nuclease cleavage product indicates a gene edit. GFP gRNA-transduced...The basis of the Surveyor assay is that Surveyor nuclease cleaves double stranded DNA (dsDNA) at basepair...mismatches at the site of the edit. Using Surveyor nuclease followed by gel electrophoresis, these mismatches...Before digesting the DNA duplexes with Surveyor nuclease, save some of the duplex and run it as an undigested...BRAF sites were edited in A549 cells. Surveyor nuclease was used to digest (+) mismatches in DNA duplexes...

...GreenGate are type IIS restriction endonucleases. They are like other endonucleases except that they cut at arbitrary...be very careful with the type IIS restriction endonuclease of choice, BsaI, which can be very sensitive... They finally traced the problem back to the endonuclease. “It’s just a sensitive protein,” he says. “...

...tagging Lentiviral capsid-based Cas9/gRNA ribonucleoprotein delivery Cytosine deaminase for RNA editing...bioRxiv Find the ORANGE toolkit   Cas9/gRNA ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle...bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the...a lentivirus-like particle. Read the paper in Nucleic Acids Research Find the gRNA and packaging plasmids...

...Note: hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com...improved the CGBE: DNA polymerase D2 (POLD2), exonuclease 1 (EXO1), and RNA binding motif protein X-linked...Repair A cellular process in which incorrect nucleobases are removed from the backbone, creating abasic...further repair.   Abasic site A DNA site with the nucleobase removed; also called an apurinic / apyrimidinic...

... from E. coli is the presence of the protein endonuclease I encoded by the gene endA. Nowadays, the most...gene has been removed/mutated to neutralize the nuclease activity. For strains that still carry this gene...introduced in the purification protocol to remove endonucleases activity. For these reasons choosing the correct...most downstream processing. On the other hand, nuclease free water is a great option because it provides...

...complex minus its nuclease component, Cas3, while Type V-K CAST systems use a nuclease-deficient Cas12k..., Tn7-like transposons captured and repurposed nuclease-deficient CRISPR-Cas systems to help them spread...Gelsinger et al., 2024). In either type of CAST, the nuclease-deficient CRISPR-Cas system identifies and binds...

...CRISPR-associated.” The most famous of these proteins is Cas9 nuclease, but there are many other Cas proteins as well...the ability to use an RNA guide to target other nucleic acids—usually DNA—to cut, nick, or bind. DNA CRISPR...genetic record of previously encountered foreign nucleic acids. The unusual sequence of these arrays, with...for studying CRISPR. Spacers: Copies of foreign nucleic acid sequences in the CRISPR array that will become...

...on Precision Genome Editing with Programmable Nucleases. Check out #KSgenome on Twitter if you missed ...would open not with an introduction to CRISPR nucleases, but with a session on DNA repair. What stuck ...biophysics can help enhance HDR. CRISPR 101: Ribonucleoprotein (RNP) Delivery The RNP delivery method consistently...Mechanisms of precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi...

...widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific...and follow the DNA sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires... 2-D linear molecule snaking through our cells’ nuclei--our chromatin is comprised of thousands of loops...provide a 3-D structure for packing genomic DNA into nuclei, but are also thought to play a key role in the...

...Because it contains a nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind cognate ...cell. When cellular mRNAs are digested with ribonucleases, ribosomes protect the segments of mRNA to which...Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling.” Science...

...particles enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear envelope during...during cell division to enter the nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus...

...through nuclear localization signals which can traffic their viral particles inside the nucleus, without...access to the host genome requires breakdown of the nuclear envelope, which occurs during mitosis. The most...PMCID: PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human immunodeficiency virus type 1 preintegration...

... the miCube, SQUID (Hongquan, et. al, 2020) or openUC2 (Diederich et. al., 2020) aim to make microscopy.... There are a number of new platforms (such as openUC2, which I am a co-founder of), that aim to support...microscope or open-source modular optical toolbox openUC2 are trying to overcome this hurdle by taking these...He is co-founder of the open-hardware startup “openUC2” and focuses on bringing cutting-edge research ...

...a CRISPR repeat array near a putative effector nuclease. The Cas13d proteins they identified have little...identified Cas13a-c orthologs, but they do include HEPN nuclease domains characteristic of the Cas13 superfamily...great results with CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293 mCherry ...each of four targets, including two mRNAs and two nuclear long non-coding RNAs. One interesting twist to ...

...identifies plasmid features that differ by only a few nucleotides, such as the fluorescent proteins EGFP and mEGFP...listing commonly used primers detected in a given nucleotide sequence. The table includes primer names, sequences...determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases...databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2. See this...

...Cpf1 CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease size spCas9...and doesn’t require the expression of other endonucleases, like Csy4, or the inclusion of processing signals...Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell,163(3),...

... works best for genome editing  with an active nuclease.  Read the paper in Molecular Cell Find the CasMINI...Programmed genome editing by a miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature...various delivery methods, including plasmid, ribonucleoprotein, and AAV.  Read the paper in Nature Chemical...protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read...