Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 359 results for: Nov

Showing: 101 - 120 of 359 results

  1. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. (2023). Various AAV...s41434-019-0075-6 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. (2023). Various AAV...

  2. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...-chimeras) is that they undergo normal protein turnover. FP-chimeras are continuously synthesized and ...virtually impossible to determine specific protein turnover or temporal expression using standard FP-chimeric...stability, chromophore maturation rate at 37 °C, turnover rate, and the level of background fluorescence...scientists can track single protein localization, turnover, and trafficking, as well as organelle trafficking...

  3. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ... a diverse range of perspectives and sparking innovative thinking that may benefit your current project...mindset that can lead to unexpected discoveries and innovative solutions. Allowing for serendipity in your lab...confines of a predefined path, opening doors to novel ideas and approaches. Moreover, the ability to embrace...

  4. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...destroy the invader. Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/...programmable platform well-suited for various innovative biological and translative technologies. Addgene...(12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org...

  5. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...different viral production strategies or developing novel methods for data analysis, our team’s goal is to...Palsson, 1991; Oh, 1993) and increased yields in adenoviral (Coroadinha, 2006) and retroviral production ...phase, but not the production phase, improves adenovirus production." Journal of biotechnology 157.1 (...

  6. What the HEK?

    Type
    Blog Post
    ...Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment...transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. (2002...Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and Troubleshooting Additional...

  7. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...is the first 3 books of a larger epic of fantasy novels. Set in a world where 1000 years ago the hero lost...     - Jason Snair, Product Designer Sarum: The Novel of England by Edward Rutherfurd "A 10,000 year history...undergraduate degree in English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been...

  9. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...She is particularly interested in exploring how innovative tools influence the direction of scientific research...    Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT Sloan School of Management...performance, and randomized control trials in innovation. Visit his website at: http://www.neil-t.com/...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...includes four promoters, six fluorophores with nonoverlapping emission spectra (N- and C-terminal tags for...using these tools in virtually any vertebrate. This novel AAV approach allows you to target and functionally...Podcast Segment on This New AAV Tool    mCyRFP1: Novel fluorescent protein that enables imaging of two ...Lin and Ryohei Yasuda have teamed up to develop a novel monomeric cyan-excitable red fluorescent protein...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...Berkeley, provides a new approach for generating novel genetic variants in bacteria. Read on to learn about...of EvolvR The Dueber Lab used EvolvR to discover novel genotypes that result in a spectinomycin resistant...spectinomycin, rpsE. These results not only uncovered novel resistance genotypes, but also provide a better ...

  12. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors...virus in your research - a primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time...

  13. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...; synthetic biology is required to achieve this novel and unique phenotype. It’s an unexpected use of ...creating new forums and opportunities for open innovation in plant synthetic biology. In our case, we selected...off patent, or not patented, and building our own novel pieces where applicable. We will also be working...

  14. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...one, is a sterling example of collaboration and innovation between legal and scientific fields.” Thanks ... every day for their dedication, flexibility, innovative thinking, and openness to team work.   Company...positions. We have an awesome talent pipeline. Turnover at Addgene is about half of what is standard in...

  15. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...Research Strategy highlights i) the significance and innovation of the project and ii) how you, the expert, will...writing a proposal to test hypotheses and develop novel technologies! If data from a publication or preliminary...funding. Convince the readers why and how your innovation will address a variety of challenges and impact...

  16. A Look at Addgene's QC Process

    Type
    Blog Post
    ...assembled into a circularized plasmid sequence de novo (without the use of a reference sequence) using ...the plasmid, QC scientists will first align the de novo NGS result to the deposited sequence(s) in SnapGene...if one was provided. If no reference is given, de novo assembly in Geneious can still provide additional...

  17. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). The least ...is usually classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate...integrate into the host’s genome. However, some adenovirus strains are known to cause respiratory diseases ...

  18. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...replicate itself and depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient...a “helper-free” system, in which the required adenoviral helper genes are supplied on two different packaging..., J. (1999). Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production...

  19. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...expressing your gene, especially if you are studying a novel gene, is not always a straightforward process. Rather... of interest, scientists have instead developed novel methods to co-express your gene along with a reporter...more time developing the clever experiments and innovative solutions necessary for advancing the field. ...

  20. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...co-founded by Kaspar, acquired by Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies... in tackling the root cause of Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource...
Showing: 101 - 120 of 359 results