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We narrowed to 353 results for: Plat

Showing: 101 - 120 of 353 results

  1. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...Method Notes Quantification of a process Plate-based assays (plate reader) Detect the average amount of ...fluorescence across a population of cells (i.e. in a plate well). Use to track a process (e.g. enzyme activity...

  2. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...enough time to completely digest the template DNA. Note—templates must have been isolated from methylation...sensitive restriction enzyme DpnI to remove the templates. Digestion products are then transformed directly...

  6. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...can be counteracted by growing the cells on solid plates, where each transformant forms its own colony, ...less of a concern, but Addgene still recommends plating the transformation mixture on solid media. The ...The colonies are then scraped off the plates and pelleted for DNA extraction. Prepare library product for...

  7. How to Lead a Great Meeting

    Type
    Blog Post
    ...communicate outcomes to the rest of their team. Here's a template for project meetings. Team Meetings: For standing...members still have their voices heard. Here's a template for team meetings. One-on-Ones: Managers should... agenda template...

  8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...concluded “Aspirin promotes autophagy, inhibits platelet aggregation, and potentiates the effect of PD-... UCSF Immunoprofiler - a human biopsy analysis platform focused on analysing immune measures and correlating...with tumor mutations and clinical responses. This platform has the potential to become an open-source pipeline...

  9. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...results? Or considered why DpnI will degrade your template DNA from a PCR reaction but not the newly synthesized...no methyltransferases in the PCR reaction). The template plasmid, on the other hand, should be derived ...with DpnI, only the non-mutated and methylated template is destroyed leaving behind a pool of mutated ...

  10. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... the cell with a mutant template and fooling the cell into using that template for repair. By using these...the two ends back together or using a homologous template provided by the sister chromosome. As cells take...

  11. Synthetic Biology - Overview

    Type
    Collection
    ...Jaramillo Lab Riboregulation Keasling Lab BglBrick Platform , Terpenoid Production , and Propionate Induction...

  12. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    .... CombiGEM-CRISPR: The marriage of two simple platforms CRISPR has revolutionized how we decode the genome...regulation in biological systems. The CombiGEM platform provides a means to create barcoded gRNA libraries...Wong et al., 2016). With CombiGEM and CRISPR platforms now being integrated, we look forward to the realization...

  13. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ... a fluorophore or tag. To utilize HDR, a donor template containing the desired sequence flank by homology...Nanoblades which carries a gRNA, Cas9, and a HDR donor template DNA can be generated by incubating Nanoblade particles...facilitated HDR with either a ssDNA or dsDNA donor template. In vivo editing of mouse zygotes and mice with...

  14. A Look at Addgene's QC Process

    Type
    Blog Post
    ...Nobody’s perfect! This is a platitude I’m sure we have all heard. It’s a truth that extends to both people...sequence and assemble using the Illumina MiSeq platform, such as GC-rich regions and highly repetitive...Plasmidsaurus for long-read nanopore sequencing. This platform sequences the entire plasmid in one read and thus...

  15. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... 2014). The second was an in vitro paper-based platform that allowed gene circuits to be deployed out ... had been working on advancing the paper-based platform toward a more field-ready format when the Zika...we say the timeline to develop our paper-based platform into clinically relevant diagnostics for the Zika...

  16. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...that grew on a selective plate versus the number that grew on a non-selective plate. Most bacteria tested...glmS gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for...

  17. Pooled Library Amplifications

    Type
    Blog Post
    ...liquid media or solid media (on plates). In general, amplification on plates can help to ensure that diversity...library varies significantly in plasmid size). On plates, each transformed bacteria will be its own colony...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...double-stranded break. HDR copies the sequence from a repair template with flanking sequence homology for error-free...systems, and NHEJ will be favored even if a repair template is present. HDR also presents a cloning dilemma...’s become clear that the power of this editing platform lies in the diversity of nucleases and their applications...

  19. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ... to guides in the Broad’s Genomic Perturbation Platform Avana library (used by the Cancer Dependency Map...and Adam Brown from Broad's Genetic Perturbation Platform to design a new library that is agnostic to ancestry... are working with Broad's Genetic Perturbation Platform to modify the CRISpick algorithm to prevent guides...

  20. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...binding site (PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the ...
Showing: 101 - 120 of 353 results