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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 443 results for: RELA

Showing: 101 - 120 of 443 results

  1. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...al. found that translation efficiency is well correlated with codon bias in both E. coli and S. cerevisiae...with specific secondary structures in 10 closely related yeast strains. Adaptation to changing conditions...theory, this is not as easy as it sounds. Even for relatively short peptides, there can be many possible ways...non-exhaustive list includes: Codon abundance relative to cognate tRNA abundance Repetitive sequences...

  2. Viral Production at Addgene

    Type
    Blog Post
    ...of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins...SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed...Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total protein present in the sample. ...vectors consist of full particles (white arrowhead) relative to empty particles (black arrowhead). Scale bar...

  3. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...AAV-PHP-B, it can be produced with high viral yields relative to AAV9. At lower viral titers than AAV-PHP.B ...when paired with the ubiquitous CAG promoter, is relatively biased toward L5 and inhibitory neurons and astrocytes...C57BL/6J and BALB/cJ mice through LY6C1, with relatively even distribution of target gene expression (...Spatial transcriptomics shows that its tropism is relatively more biased toward excitatory neurons than AAV-PHP.eB...Evolutionary tree depicting the phylogenetic relationship of common research species. Among these species...DNA levels (~25-fold and ~4-fold respectively) relative to AAV9 in the pigtail macaque brain slices. Similarly...

  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...genotyping, is critical to demonstrating causal relationships between genotype and assayed phenotype. Verifying...and T7E1 have been widely adopted due to their relative simplicity and low cost. The problem with these... detection rates mean the investigator can be relatively certain that their samples don’t have off-target...take into account other elements, including those relating to DNA structure (e.g. epigenetic modification...

  5. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    .... Western blots are frequently used to compare relative levels of protein expression between cell types...protein can be used in experiments to compare the relative amount of protein present in a series of samples...interest from among the mixture and to measure the relative amount of your protein of interest on or in these...signals (up to a dozen or more!) to measure the relative or absolute amount of the expression of multiple...

  6. Deep Dive: qPCR

    Type
    Blog Post
    ...You know qPCR is most commonly used to measure relative quantification of mRNA, which can be used as a...reported to work well in the Lightcycler while being relatively easy to multiplex (Thellwell, 2000). See Table... Accuracy is not as important as precision for relative quantification. A consistent 5% positive error...Accurate and statistically verified quantification of relative mRNA abundances using SYBR Green I and real-time...

  7. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...of the method. One observation was an inverse relationship between deletion size and frequency [3]. There... DNA function is typically inferred based on correlative biochemical characteristics and ectopic reporter...   Like the video? The Orkin and Bauer lab's related paper describes their methods for CRISPR design...

  8. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...to detach from the constant stream of thoughts related to lab research and gain fresh perspectives. Such...encourages exploration of tangents or seemingly unrelated ideas. It's beneficial to carry a notebook at ... identify areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives...

  11. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...the reaction on an agarose gel to determine the relative sizes of the resulting DNA fragments. Restriction...) shows 3 possible plasmid conformations, with relaxed and nicked marked with asterisks (*). When the ...conformations the most abundant being: supercoiled, relaxed and nicked. If your digest lanes look like your...

  12. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...is software product management, and how does it relate to science? In the simplest of terms, a software...project management  Bolster your skills in strong relationship-building and influence. You’ll need a large ...large deal of influence and strong relationship-building in order to rally many stakeholders behind a common...

  13. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Cancer and the Immune System: Deciphering the Relationship” by our guest blogger Subhadra Jayaraman, a ...platform focused on analysing immune measures and correlating them with tumor mutations and clinical responses...Cancer and the Immune System: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids...

  14. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...our engineers (Fig. 2). Creating this guide was relatively easy. I took a picture of virtually every container...important documentation, including SOPs or project-related documents, we define new and potentially ambiguous...transferable skills Find all of our science career related blog posts Read our science communication blog ...

  15. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...widefield imaging fluorophores are excited with relatively low light levels (for a prolonged time). Other...experiments. As the photobleaching rate is not linearly related to power, the conclusions reached at high power...application (Goedhart et al, 2012). The third issue relates to the environment of the fluorescent protein. ...

  16. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...future prospects. Among the topics discussed, many related to the structure of the biomedical workforce. Quotes...results, not scientists”  These comments are well correlated with published data. For example, in 2010, less...grants through the Sloan foundation for postdoc-related data collection and analysis. He is also a strong...

  17. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...scientists are aware of the benefits of coaching relationships and do not know where to look for a coach. Many...thinking about what your goals are for the coaching relationship. Do you want to change jobs? Grow your professional...the coach to define how you will know that the relationship has been successful – what are the desired outcomes...

  18. Working with Nuclear Receptors

    Type
    Blog Post
    ...Within the animal kingdom, this information is relayed through factors (both of endocrine and dietary ...systematic effort to compile NR- and coregulator-related global gene profiling datasets in to our data mining...coregulators. We look forward to growing this important relationship with Addgene for the benefit of the entire research...

  20. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... HR carefully because sometimes companies have relaxed dress codes for interviews. You can also ask HR... know up front how you plan to take questions.  Relax!  You wouldn’t have made it to the interview if ...position. So prepare for the interview, but then relax.  Additional resources on the Addgene blog: Tips...
Showing: 101 - 120 of 443 results