...In summary, this cell free system allows for a relatively simple, controlled “one-pot” reaction capable...

...approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed...experiments with more detailed investigations into the release of DNA post-cut. Using substrate DNA labeled on...the cleaved duplex, while the fourth strand (the released strand in Figure 1 below) was free to anneal to...single stranded donor DNA: Complementary to the released strand, 127bp in length, With 36bp on the PAM-...

...the reaction on an agarose gel to determine the relative sizes of the resulting DNA fragments. Restriction...) shows 3 possible plasmid conformations, with relaxed and nicked marked with asterisks (*). When the ...conformations the most abundant being: supercoiled, relaxed and nicked. If your digest lanes look like your...

...make your own. In my next post, I’ll explain the relatively straightforward process of generating your own...

...adding more terms.  Weighting We determined the relative importance of different bits of information for...completeness of our information, and the accuracy and relevance of our search results. The footer and improved...search engine to return only the filters that are relevant to a specific query and category. For example,...shortcuts to specific catalog items as well as related items (for example, a kit that contains a plasmid...

... the vaccine, we've been able to measure their relative ability to neutralize.”   Figure 2: Sera...resources on the Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources...

... workhorse adeno associated virus (AAV) shows relatively low retrograde transport efficiency in most serotypes...

.... A unique evolutionary feature of K. phaffii, relative to S. cerevisiae, is that it predominantly uses...enabling study of human-relevant pathways (Liu et al., 2017). Beyond human relevance, yeast have many applications...following decades, Phillips made the decision to release the K. phaffii expression system to the general...glucose or glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily it...

...epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays due to only having...antibody per target just isn’t enough and we recently released Anti-GFP [N86/8R] as another option for targeting...tested hfYFP in expansion microscopy as well as correlative light and electron microscopy and observed high...

...trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al., 2022).    ...Antidrug Antibody Formation in Oncology: Clinical Relevance and Challenges. The oncologist, 21(10), 1260–1268...

... a given disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based SDM has lagged...directed mutagenesis methods Current SDM methods rely on in vitro assembly. To make a point mutation, ...some of which are proprietary. These methods also rely on ssDNA ends to match up regions of homology, so...better one. Trehan et al. wondered if, instead of relying on in vitro assembly, they could make the E. coli...

...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...significantly improve the reproducibility and reliability of science publications. Peer reviewing To make...make sure your manuscript is perceived as a reliable source of information, it is best to submit it to...

...colored product that accumulates over time which is relative to the amount of enzyme present in each well. ... categories: Quantification - used to quantify relative amounts of protein. Includes: Western blot ELISA... western blots or ELISAs, are used to quantify relative amounts of protein. Western blot Western blots...proteins separated by size on an SDS-PAGE gel. Though relative quantification can be done, western blots primarily...referred to as housekeeping genes). This allows for relative comparison of expression between different samples... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain...

...populations and to study relative protein expression. Its reliability will be entirely dependent on the selectivity...and the abundance of the epitope in the sample, relative to cross-reactive antigens – that is, an antibody... are relying on correlations between actual antibody labeling and expected labeling. Correlation cannot...practice to combine correlation with other approaches when possible. Finally, correlation of -omics data ... antibody selectivity even more challenging. Correlation with RNA or Proteomic data from multiple cell...identification of the cell types of interest. By correlating your data sets, you can increase confidence that... of an orthogonal approach to understand the relationship between antibody staining intensity by flow ...

... present, it will repress expression of mKate2 relative to EBFP2. Weiss’s group used these reporters to... it inactive, thus inhibiting neurotransmitter release. How is this light-mediated? The researchers split...visualizing the location and function of microbes rely heavily on fluorescent protein genes. These optical...

...can be very helpful if you're trying to find a relatively rare protein in an experiment with many proteins...

...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...identifies proteins solely by weight and size, relying on the signaling molecule solely asmeans of visualization...assays.  A brief note Since the indirect method relies on multiple secondary antibodies attaching to a...

...sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is... Links to relevant resources The homepage displays a graphic describing CasIDs and relevant properties... What are the features? CasPEDIA has collected relevant information on all the Cas enzymes you know and...

...induce a lower inflammatory response within hosts. Relative to the other viruses discussed in this article...lentivirus and gamma-retrovirus are so closely related, their required components are very similar. While...

...mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated...used to achieve transient, high expression of the relevant cargo without the fear of random genomic integration...