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We narrowed to 601 results for: Ret;

Showing: 101 - 120 of 601 results

  1. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...evaluated set of values.  However, to evaluate the interpretation of the experiment would require access to each...no one is suggesting all data be stored in a retrievable format, we too rarely preserve “raw” data of ...journal repository, they are likely too large to be retrieved by others. Other new data initiatives are also...addition to enhancing veracity of collections and interpretation of data, publishing complete data, rather than...

  2. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...Evaluating Efficiencies of Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org...(2013) Effective delivery of large genes to the retina by dual AAV vectors. EMBO Mol Med 6:194–211 . https...truncated proteins are safe and effective in the retina of a mouse model of Stargardt disease. Hum Mol ...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...

  3. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...Filonov et al., 2013). FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1...indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were...optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600 (2018). Shemetov, A.A...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with...cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification...

  5. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...or PNS transduction, cell type specificity, or retrograde transport in neurons. (b) Genetic cargo can be...typically administered in the lateral tail vein or retro-orbital sinus. IT injections are administered in... Chen et al., 2022 PAL2 CNS: cerebrum; neuroretina Macaques N/A Stanton et al., 2023 AAV-MDV1A...could be used to target endothelial cells in the retina and spinal cord (Krolak et al., 2022). Systemic...macaques. It also effectively transduces the neuroretina. PAL2 detargets the liver and has increased DRG...Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu,...

  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically...Campbell Zinc FRET-based zinc sensor for imaging in live cells (CALWY) Genetically encoded FRET sensors to...-2 Zn2+ FRET sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for...Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately colored FRET sensors for simultaneous monitoring...cyclic AMP) FRET sensors of cAMP levels for imaging in live cells Fourth-generation epac-based FRET sensors... for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion in Single beta Cells...Pyruvate Pyronic FRET sensor for pyruvate Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for ...

  7. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...in real time Proteins involved in endoplasmic reticulum quality control navigate a large and interconnected...factors present unique challenges for study. These secreted signaling proteins regulate vital cell and tissue...subtle changes in co-receptor dynamics, including NanoBRET assays to monitor the binding of fluorescently-labeled..., J., & Hill, S. J. (2021). Use of NanoBiT and NanoBRET to monitor fluorescent VEGF-A binding kinetics...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...remember that some serotypes can be transported retrogradely and/or anterogradely along the axon. Others,...your gene and could lead to toxicity or trouble interpreting your results, while a low transduction efficiency...Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate...Introduction to AAV Viral Vectors 101: The AAV Retrograde Serotype Viral Vectors 101: Viral Applications...

  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins...Endoplasmic Reticulum ER retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention...38770 pEF.myc.ER-E2-Crimson Endoplasmic Reticulum ER retention signal E2-Crimson Benjamin Glick 36204 ...36204 pmTurquoise2-ER Endoplasmic Reticulum ER retention signal mTurquoise2 Dorus Gadella 137804 ER-mNeonGreen...Endoplasmic Reticulum KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic Reticulum KDEL ...Endoplasmic Reticulum BiP ER signal sequence BFP Gia Voeltz 61807 Rtn4a-GFP Endoplasmic Reticulum Rtn4a AcGFP...Endoplasmic Reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic Reticulum Sec61 BFP Gia...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...to not have any effect on each other, they have discrete effects on the dependent variable, and those variables...others. Each test has different likelihoods of returning false positives/negatives, but they all generally...numerical value relative to quantiles derived from a theoretical distribution. If your data is normally distributed...130 (2012). 10.1097/SMJ.0b013e3182498ad5 Curran-Everett, Douglas. Explorations in statistics: statistical...

  11. Bacterial Expression Systems

    Type
    Collection
    ...Protein Timers Return to top Protein Interactions Förster resonance energy transfer (FRET) and bimolecular...tdTomato-pBAD mAmetrine1.1 (donor) tdTomato (acceptor) FRET/Dual FRET Robert Campbell Michael Davidson , Nathan Shaner...mCitrine-pBAD mTFP1 (donor) mCitrine (acceptor) FRET/Dual FRET Robert Campbell , Michael Davidson 54571 54856...mRuby2 (acceptor) FRET Michael Davidson 18856 pGWF1 ECFP (donor) Venus (acceptor) FRET Wolf Frommer 65616...Citrine (acceptor) FRET Wolf Frommer 65617 pFLIP42 mCerulean (donor) Citrine (acceptor) FRET Wolf Frommer 65618...expression upon protein–protein interaction. The cpFRET - FRET-based Biosensors Kit (Addgene #1000000021) is...Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization To determine the location...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PSPS1, PTP, REG RETN resistin ADSF, FIZZ3, MGC126603, MGC126609, RETN1, RSTN, XCP1 RETNLB resistin like ...ULBP4, bA350J20.7 RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L... 3 RAET1N Return to top BCR Signaling B cells mediate humoral immunity through the secretion of antibodies...chromogranin A (parathyroid secretory protein 1) CGA CHGB chromogranin B (secretogranin 1) SCG1 CKLF chemokine-like...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor A/guanylate ...cyclase A (atrionatriuretic peptide receptor A) ANPRA, ANPa, GUC2A, GUCY2A, NPRA NPR3 natriuretic peptide...

  13. Luciferase Plasmid Collection

    Type
    Collection
    ...Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase reaction...Antares ) for in vivo imaging. NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored...Kit , and G-CASE : BRET-based sensors for reporting on GPCR activity. AIMTOR : BRET biosensor for live...kDa and 36 kDa, respectively). And they are not secreted, so cells must be lysed or the substrate must ...to measure luminescence. Gaussia luciferase is secreted and more stable, enabling time course experiments... kDa), very bright, and ATP-independent, and a secreted version of the protein is available, making it...

  15. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...Wilson New serotype AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? ...marker, a Flp-dependent channelrhodopsin in the retrograde serotype, and a dTomato under the control of ...

  16. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...displays enhanced transduction of the retina following subretinal administration and enhanced transduction...transduction and lateral spread in the retina following subretinal injection. It was identified by John ...transduction and lateral spread in the retina following subretinal injection as compared to the parental...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...demonstrated to have enhanced transduction of the retina following intravitreal injection in mice and marmosets...trpYF) serotype shows enhanced transduction of the retina following intravitreal injection in mice and macaque...2009. High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther...

  17. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ... user, instead, don’t fret; we have backgrounds for you as well! If you use FRET, you might be interested...Fluorescent Proteins blog topic to learn more about FRET and other techniques using FPs! Otherwise, check...

  18. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...For example, searching the term "breast cancer" returns 40 screens performed in 26 different breast cancer...and other details relevant to screen result interpretation. The analytical method for score assignment...maintain consistency with published screen interpretations and allows users to query screen data without...

  19. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...find this hard so get used to it by making it a concrete goal to say “thank you, job well done” to one ...not the person - intent is usually good. Don’t pretend something went well when it didn’t… but don’t “...compare with your own perception? Tell me your interpretation of my feedback to you. What are We Learning...

  20. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Calretinin [L122/68R] Calretinin Human Mouse IgG2a 177469 Anti-Calretinin [L122/6R] Calretinin Human...220384 Calretinin [L122/110R] Calretinin Human Mouse IgG2a 220385 Calretinin [L122/117R] Calretinin Human...220386 Calretinin [L122/132R] Calretinin Human Mouse IgG2a 220387 Calretinin [L122/133R] Calretinin Human...220388 Calretinin [L122/136R] Calretinin Human Mouse IgG2a 220389 Calretinin [L122/20R] Calretinin Human...-CALB2/calretinin [L122/68R-2b] CALB2/calretinin Human Mouse IgG2b 206642 Anti-CALB2/calretinin [L122/...-CALB2/calretinin [L122/68R-1] CALB2/calretinin Human Mouse IgG2a 206691 Anti-CALB2/calretinin [L122/6R...Theta Human Mouse IgG2a 188159 Anti-Calretinin [L122/144R] Calretinin Human Mouse IgG2a 188160 Anti-TASK1...
Showing: 101 - 120 of 601 results