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We narrowed to 583 results for: Ret;

Showing: 101 - 120 of 583 results

  1. Plasmid Collections

    Type
    Collection
    ...Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated Topics ...Foundation Neurodegeneration Research Collection Rett Syndrome Plasmid Collection and Resource Center ...

  2. Optogenetics Plasmid Collection

    Type
    Collection
    ...Gene/Insert Vector Type Promoter PI Publication Return to top Optical Switch Plasmids Browse, sort, or...Gene/Insert Vector Type Promoter PI Publication Return to top Do you have suggestions for other plasmids...

  3. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...produced in high-titer retroviral stocks generated by the transfer of a retrovirus into the packaging cell...CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector background Until the development of viral...cells, labs use viral vectors and particularly retroviral vectors because they target a broader range of...into the genome is more efficient. The first retroviral vectors used were derived from the Moloney Murine...MoMLV, lentiviruses, a separate genus of the Retroviridae family including human immunodeficiency virus...transfer vector. Lentiviral vectors Although retroviruses are still in use, considerable efforts have ...possibility that mutation or recombination with human retroviruses could lead to RCL. Regarding transduction efficacy...

  4. Serotype Testing AAV

    Type
    Collection
    ...AAV9 37825-AAV9.T 20 µL $ 140 Add to Cart AAV Retrograde 37825-AAVrg.T 20 µL $ 140 Add to Cart pAAV-hSyn-EGFP...AAV9 50465-AAV9.T 20 µL $ 140 Add to Cart AAV Retrograde 50465-AAVrg.T 20 µL $ 140 Add to Cart *Each viral...

  5. Antibody Guide

    Type
    Collection
    ... Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects... your samples using permeabilization, antigen retrieval, and/or sectioning. Block to reduce non-specific...upwards of twenty. Laser and software capacity theoretically allow for roughly fifty different fluorophore...

  6. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ..., but at their core they can all be boiled down pretty simply – they are systems that measure biological...fluorescence in the presence of calcium. This includes FRET-based sensors such as Twitch. While calcium is king...calcium dynamics in the cytosol and sarcoplasmic reticulum are of interest to muscle biologists, while neuroscientists...Neurotransmitters Many canonical fluorescent as well as FRET-based biosensors have been engineered to detect ... vs. low in the cell the donor and acceptor of a FRET pair on actinin will produce different signals based...For one, many viral platforms, such as AAV or retrovirus, can either permanently integrate and/or express... sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we’ve got you covered...

  7. Adenovirus Plasmids

    Type
    Collection
    ...100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not integrate into the host...into single full-length adenovirus genomes Bunz Return to Top Do you have suggestions for other plasmids...

  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...specific response cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase...Synchronization of secretory protein traffic in mammalian cells The journey of secretory proteins, from their...that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation of varying...the Institut Curie in Paris, has developed the retention using selecting hooks (RUSH) system. How does ...proteins fused to a streptavidin core that are able to retain reporter proteins fused to the streptavidin-binding...reporters which can then be tracked along the secretory pathway (Figure B). The RUSH system has already...

  9. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Callaway EM. Retrograde neuronal tracing with a deletion-mutant rabies virus. Retrograde neuronal tracing...that cell, and nascent RABV cross synapses to retrogradely infect neurons that project to the starter cell...occurs, subsequent cycles of viral replication and retrograde transmission occur, spreading the virus through...RABV G protein is necessary and sufficient for retrograde transmission (Etessami et al., 2000, Wickersham...released from the starter cell and transmits retrogradely to first order neurons, via entry mediated by...Conzelmann KK, Lepier A, Götz M, Berninger B. Retrograde monosynaptic tracing reveals the temporal evolution... for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...

  10. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...the interpretation of the parameters we infer, since scientific productivity is better interpreted as ...request activity appears to return to normal. The model fit these two discrete phases with an estimate of... impacted. The inferred weekend effect is, in retrospect, generally more dramatic than the COVID-19 effect...remind the reader that this is not a causal interpretation -- the effect would generally not be the same...just one lab, which limits how broadly one can interpret our analysis of COVID-19’s effect on scientific...instabilities in the China data, so we recommend interpreting the model results for China with more uncertainty...

  11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini et al., 2012) measures...homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay...considered a linker (figure 1). In fact, for several FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai...fluorescent protein can be truncated to increase the FRET efficiency. Creating fusions at the N- and C- termini...technology and should always be considered when interpreting results.  The recent revolution in genome editing...Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness,...PubMed PMID: 30886412. Mastop, Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in...

  12. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...interaction that increases FRET transfer between the fluorescent proteins. FRET is highly amenable to biosensor...biosensor creation, and custom FRET biosensors can be created using the cpFRET kit created by Olivier Pertz... increases in fluorescence of up to 11,000-fold. FRET biosensors are another common class of biosensors... Tsien’s lab contain two fluorescent proteins (a FRET pair) separated by CaM and M13. When calcium binds...such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples ...

  13. p53 Pathway

    Type
    Collection
    ... for this page was generated with the help of . Return to top p53 Pathway - Gene List Click on a name ...TSC2 Tuberous sclerosis 2 TSP1 Thrombospondin 1 Return to top References Unravelling mechanisms of p53...

  14. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...Cameleon-based FRET sensor D1ER, and GCaMPER. Bonus: There are also Cameleon-based FRET sensors available...CEPIA) or through FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter...fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural...available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded optical space...buffering and to increase sensitivity), and is a great FRET sensor option if you have the setup necessary for...

  15. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...mScarlet's emission via FRET.  Image from Beck et al, 2019 under CC BY 4.0.   FRET Chemigenetic Sensors...FP-fusion to rhodopsin resulted in electrochromic FRET capable GEVIs, with rhodopsin quenching the added...and available with red-shifted fluorophores (find FRET capable GEVIs here!). The only issue with these ...variety of different components, but all contain a FRET pair, a genetic component, and a small molecule ...modulate the degree of quenching of the fluor via FRET. This system is particularly powerful for in vivo...Optogenetics Fluorescent Proteins 101: Introduction to FRET ...

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...plasmid addiction systems” require dividing cells to retain plasmids - if a cell does not inherit plasmid due...the more stable toxin. Cells only survive if they retain the plasmid. Since their discovery, plasmid addiction...by the gene of interest. As a result, cells that retain the ccdB gene die off and a higher percentage of...systems onto plasmids allowed cell populations to retain more of these plasmids as compared to those without...after 24 hours, the unstabilized plasmids were retained in less than 10% of cells while plasmids containing...containing the plasmid addiction system were retained in 75% of cells. They were able to apply this system...adapted by researchers for many uses when plasmid retention without selective pressure is necessary or for...

  17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...Desfarges & Ciuffi, 2012). One family of viruses, retroviridae, require integration for their life cycle to... during production (read our blog post on the retroviral life cycle for a more detailed description). ...concerning in clinical settings, as evidenced by retroviral gene therapy trials for X-linked severe combined...remember: You can never trust a lentivirus with secrets... Because they tend to insert themselves into ...immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate...lessons from provirus and non-integrated DNA. Retrovirology, 1, 13. https://doi.org/10.1186/1742-4690-1-...resources on the Addgene blog Viral Vectors 101: The Retroviral Life Cycle Quick Guide to All Things Lentivirus...

  18. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...Christian Gram realized that certain stains were retained only in some species of bacteria. The stain colored...classification system. Eventually, any bacteria that retained the stain became known as gram positive, and the...and the ion interactions that cause the stain retention in gram positive bacteria was discovered in 1983...bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood the heat-fixed smear... thin inner peptidoglycan membrane is unable to retain the stain, leading to its decolorization. In contrast...stained by the crystal violet dye, but is easily retained in the decolorized gram negative bacteria, staining...

  19. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...excitation profiles is vital for the accurate interpretation of FRET-based experiments. A popular combination... elevation in this excited state, the electron returns to its ground state by emitting a photon with slightly...Salari et al., 2019). Photostability, the ability to retain fluorescent intensity during exposure to light,...labeled agonists and antagonists, impacting the interpretation of experimental results. It's important to ...using fluorescently tagged ligands or intermolecular FRET for ligand-receptor interactions, require specific...tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes need to have compatible ...

  20. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...difluoride (PVDF) membrane, which is sturdier and retains the blotted protein better than a nitrocellulose...transfer, dry the membrane to maximize protein retention. Following this step, most PVDF membranes recommend...proceed with caution. Unlike PVDF, they can be returned directly to buffer without a re-wetting step. ...plan to reprobe! Just as your sample protein is retained better, antibodies will stick permanently to a...membrane once before any detection steps to avoid retaining residual antibodies. If you have already applied...membrane Alright, so you’ve dried your membrane and returned it to buffer, you’ve run and imaged your western...
Showing: 101 - 120 of 583 results