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We narrowed to 456 results for: SUP

Showing: 101 - 120 of 456 results

  1. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...Geneva (Creed et al., 2015) used optogenetics to support these existing findings by controlling neurons ... animal is placed into a tank of water with no support from below or anything to hold on to. In general..., 2007). A study from 2014 (Bahi et al., 2014) supports the relevance of BDNF in depression. The researchers...Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of microRNA124a in the hippocampus evokes antidepressants-like...

  2. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...Very often, this is what it means to be a ‘superbug’. Superbugs are often very difficult and very time consuming...it a full month without a new, drug-resistant ‘superbug’ making headlines in one part of the world or ...an important step, as you can’t walk into any supermarket now without seeing a promotion for antibiotic-free...

  3. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Joanne as she answered questions to provide moral support and to monitor user replies, but I can’t thank ...questions What the moderators did not delete or suppress were the large number of scientist posters with... basic questions and to provide a small bit of support (you are not alone!) for those going through the...see graduate and postdoc programs beginning to support diverse career path development--but the science...

  4. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...you. One such option is the CRISPR Design tool supplied by the Zhang lab. This simple website invites ...the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...you can order them yourself through whatever DNA supply company you currently use. You can also find many...selected gRNA’s, and has the bonus benefit of supplying you with primers for validating your edit in mice...

  5. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...include a note as to where readers may locate data supporting the research reported in PLOS publications. The...available for anonymous request.  This means that supporting datasets must be logically annotated and arranged... in scope. For images, the number of examples supporting the published material is a factor of practicality...over time such that prior formats are no longer supported/readable. Is the author or their host institution...

  6. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ... of two ways. First, co-expression of a suppressor tRNA (SupD) instead of the SepOTSl can encode a non-phosphorylated...protein as well as context-specific effects of UAG suppression, as described (6). To determine the stoichiometry...pCRT7 NT TOPO E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical ...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...SunTag system, named after the "stellar explosion SUperNova", is a synthetic scaffold that recruits up to ...combinations of states. Each of the integrases is supplied on its own expression plasmid or in various combinations...combinations, while the cognate att sites are supplied as individual reporter constructs or as the fully...voltage indicators: QuasAr1 and QuasAr2 (quality superior to Arch). Both QuasAr1 and QuasAr2 possess mutations...the sgRNA (i.e. TOP1 or TOP2 constructs; see Supplementary Note 3 in the article for more info) are available...homology directed repair templates (see the supplementary info from the paper for details on repair template...excitation light source. Instead, coelenterazine is supplied to the culture media as a chemical substrate for...

  8. Rett Syndrome

    Type
    Collection
    ...opens in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in ...

  9. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...s41586-025-09141-5   AAV-engineered adipocytes suppress tumor progression in cancer models By Brian O'...Figure 5: Implantation of engineered adipocytes suppresses tumor progression in cancer models. A) Experimental...(2025). Implantation of engineered adipocytes suppresses tumor progression in cancer models. Nature Biotechnology...plasmids. Nuclease-dead Cas9 (dCas9) protein is supplied separately. Results can be analyzed using either...

  10. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... found that in the right environment and with supportive mentors, being open and vulnerable about my struggles...have often provided me with much-needed emotional support during my journey. When you are hunting for labs...join, one of the vital things to look for is a supportive environment—it makes all the difference in a ...scholarship is also a national scholarship that supports students interested in pursuing a career in research...

  11. Brain Armamentarium

    Type
    Collection
    ...function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium Project below. Plasmids...

  12. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... that position themselves for super-resolution usages, such as superior brightness, photostability, and...dyes have been specifically designed for use in super-resolution microscopy techniques, such as Stimulated...provide brighter and more stable fluorescence for super-resolution applications. Other current advancements...

  13. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...genes (e.g. tagging genes representing a protein super family). To overcome these challenges, we set out...RNAs can also be purchased from various other suppliers with a typical turnaround time of 5-7 days. The...reagents could be purchased directly from commercial suppliers without investing time and labour on reagent preparation...gene-specific crRNAs and matched ssODNs from commercial suppliers of CRISPR reagents (turnaround time 5-7 days);...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... H1 Ataxia telangiectasia Didier Trono 14581 pSUPER.ATMi ATM H1 Ataxia telangiectasia Didier Trono 14666...RAB25 His T7 Parkinson's Cheryl Arrowsmith 26210 pSUPER TBK1 TBK1 H1 ALS Tom Maniatis 26365 pENTR4_FLAG... pDONR221_SLC1A3 SLC1A3 Episodic ataxia Giulio Superti-Furga 132389 AAVS1 CAG rtTA3 TauWT 2N4R-EGFP MAPT...pDONR221-SLC1A3_STOP SLC1A3 Episodic ataxia Giulio Superti-Furga 161582 pcDNA3.1(+)_Strep-Lrrk2-Myc LRRK2 ...

  15. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...Joanne Jary from Lab Support at the John Innes Centre, and submitted by the Lab Support Team at the Centre...Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford (@samtmugford)...

  16. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmid. Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors...vector does not contain Cas9, so you’ll need to supply it with another plasmid.   Figure 3: Comparison...genomic modification. In this system, Cas9 must be supplied on a separate plasmid. Multiplexing in Drosophila...from two Drosophila U6 promoters. Cas9 must be supplied on a separate plasmid. Multiplexing in E. coli...

  17. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ... NIH-funded researchers and utilizes grants to support our own initiatives, Addgene is deeply concerned...with partners across the research ecosystem to support the essential work of our scientific community ...funding changes are impacting your work and what support would be most valuable from Addgene during this...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ...pH sensor consisting of a pH-sensitive GFP (superecliptic pHluorin), a pH-stable OFP (Antares), and Nanoluc...

  19. Caltech Systemic Capsids

    Type
    Collection
    ... serotype will work in your mouse line, see Supplementary Table 3 (Link opens in a new window) . Browse...

  20. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...and professional experience are prized skills. Supervise undergrads or less experienced grads Manage a ...something big like plan a conference Recruit many supporters and mentors that will speak for you (and you ...get real feedback Write whenever you can, find supportive editors so your first drafts are always excellent...
Showing: 101 - 120 of 456 results