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We narrowed to 447 results for: SUP

Showing: 101 - 120 of 447 results

  1. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    .... But how do you find these elusive teachers, supporters and advisors? I tried to start a more formal ...for their development plans. They all found the support of the group to be intrinsic to their success. ... continue to meet regularly, providing ongoing support, advice and accountability for development goals...mentoring relationship.  Celebrate your successes and support each other as you transition to the next stage ...

  2. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ... Training (BEST) awards, which are designed to support new approaches that address the need to train our...and from what fields – are needed to adequately supply these demands? Are there other solutions, such ...University of Maryland College Park, is a strong supporter of increasing the number of postdoctoral scientists... collection and analysis. He is also a strong supporter of science Master’s programs. This will be the...

  3. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...figure must be clear, concise, meaningful, and supportive of the mission of the application and the funding...gives the writer a chance to delegate funds to supplies, personnel, equipment, travel, etc. As a first... value of costs. It is important to be aware of supply costs per year, how many graduate students and ..., etc. It may be helpful to ask your advisor to supply a budget form used in a previously funded grant...

  4. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...population. While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these...will not necessarily express EnvA unless it is supplied in trans. Monosynaptic RABV spread can be achieved...transsynaptic spread can be controlled by selectively supplying the G protein in trans only to starter cells (...replication will then occur and the G protein (supplied in trans) will be incorporated into the viral ...

  5. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...which can reduce the potential image resolution of super-resolution fluorescence microscopy. Their size also...about 2 nm, which can lead to higher resolution for super-resolution microscopy. Nanobodies can be labeled... (2015). Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native...DSHB). 1 ml of antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially...

  6. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...priorities, but many find it helpful to obtain support and ideas from those who seem to be managing. Being... When the balance tips, we need to look to for support and ideas to manage our stress. If you are saying...club, meet the neighbors and create a network of support to help with elder care, carpooling, cooking on...’ school, and with professional organizations supporting women in science and career development. I try...

  7. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...Geneva (Creed et al., 2015) used optogenetics to support these existing findings by controlling neurons ... animal is placed into a tank of water with no support from below or anything to hold on to. In general..., 2007). A study from 2014 (Bahi et al., 2014) supports the relevance of BDNF in depression. The researchers...Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of microRNA124a in the hippocampus evokes antidepressants-like...

  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...Very often, this is what it means to be a ‘superbug’. Superbugs are often very difficult and very time consuming...it a full month without a new, drug-resistant ‘superbug’ making headlines in one part of the world or ...an important step, as you can’t walk into any supermarket now without seeing a promotion for antibiotic-free...

  9. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Joanne as she answered questions to provide moral support and to monitor user replies, but I can’t thank ...questions What the moderators did not delete or suppress were the large number of scientist posters with... basic questions and to provide a small bit of support (you are not alone!) for those going through the...see graduate and postdoc programs beginning to support diverse career path development--but the science...

  10. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...you. One such option is the CRISPR Design tool supplied by the Zhang lab. This simple website invites ...the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...you can order them yourself through whatever DNA supply company you currently use. You can also find many...selected gRNA’s, and has the bonus benefit of supplying you with primers for validating your edit in mice...

  11. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...include a note as to where readers may locate data supporting the research reported in PLOS publications. The...available for anonymous request.  This means that supporting datasets must be logically annotated and arranged... in scope. For images, the number of examples supporting the published material is a factor of practicality...over time such that prior formats are no longer supported/readable. Is the author or their host institution...

  12. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ... of two ways. First, co-expression of a suppressor tRNA (SupD) instead of the SepOTSl can encode a non-phosphorylated...protein as well as context-specific effects of UAG suppression, as described (6). To determine the stoichiometry...pCRT7 NT TOPO E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical ...

  13. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...SunTag system, named after the "stellar explosion SUperNova", is a synthetic scaffold that recruits up to ...combinations of states. Each of the integrases is supplied on its own expression plasmid or in various combinations...combinations, while the cognate att sites are supplied as individual reporter constructs or as the fully...voltage indicators: QuasAr1 and QuasAr2 (quality superior to Arch). Both QuasAr1 and QuasAr2 possess mutations...the sgRNA (i.e. TOP1 or TOP2 constructs; see Supplementary Note 3 in the article for more info) are available...homology directed repair templates (see the supplementary info from the paper for details on repair template...excitation light source. Instead, coelenterazine is supplied to the culture media as a chemical substrate for...

  14. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... found that in the right environment and with supportive mentors, being open and vulnerable about my struggles...have often provided me with much-needed emotional support during my journey. When you are hunting for labs...join, one of the vital things to look for is a supportive environment—it makes all the difference in a ...scholarship is also a national scholarship that supports students interested in pursuing a career in research...

  15. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...genes (e.g. tagging genes representing a protein super family). To overcome these challenges, we set out...RNAs can also be purchased from various other suppliers with a typical turnaround time of 5-7 days. The...reagents could be purchased directly from commercial suppliers without investing time and labour on reagent preparation...gene-specific crRNAs and matched ssODNs from commercial suppliers of CRISPR reagents (turnaround time 5-7 days);...

  16. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... that position themselves for super-resolution usages, such as superior brightness, photostability, and...dyes have been specifically designed for use in super-resolution microscopy techniques, such as Stimulated...provide brighter and more stable fluorescence for super-resolution applications. Other current advancements...

  17. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...Joanne Jary from Lab Support at the John Innes Centre, and submitted by the Lab Support Team at the Centre...Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford (@samtmugford)...

  18. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmid. Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors...vector does not contain Cas9, so you’ll need to supply it with another plasmid.   Figure 3: Comparison...genomic modification. In this system, Cas9 must be supplied on a separate plasmid. Multiplexing in Drosophila...from two Drosophila U6 promoters. Cas9 must be supplied on a separate plasmid. Multiplexing in E. coli...

  19. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ... NIH-funded researchers and utilizes grants to support our own initiatives, Addgene is deeply concerned...with partners across the research ecosystem to support the essential work of our scientific community ...funding changes are impacting your work and what support would be most valuable from Addgene during this...

  20. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...and professional experience are prized skills. Supervise undergrads or less experienced grads Manage a ...something big like plan a conference Recruit many supporters and mentors that will speak for you (and you ...get real feedback Write whenever you can, find supportive editors so your first drafts are always excellent...
Showing: 101 - 120 of 447 results