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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 581 results for: Vit

Showing: 101 - 120 of 581 results

  1. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...for in vitro cleavage, but they improve cutting efficiency and are important for robust activity in cells... helps limit off-target activity. What next? It’s not actually clear! In vitro, Cas9 holds tightly to ...cells (Jiang & Doudna, 2017). Within an interior cavity, Cas9 grips nucleotides 1-10 of the guide RNA, ...the HNH domain also allosterically controls RuvC activity through a hinge region between the two (Jiang ...finish:   Figure 9: Cartoon summary of Cas9 activity. The apo enzyme is shown in the open conformation...

  2. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...voltage sensitivity of the available VSDs has been successful even at the single neuron level in vitro. In...function depend on potential energy. Cellular activities like action potentials, muscle contractions, ...effective at the single cell level as they are in vitro. Factors like photobleaching, lack of red-shifted...genetically encoded component is delivered in vivo or in vitro and a small molecule is added exogenously to measure...red fluorescent voltage sensor to detect neural activity. Sci Rep 9, 15878 (2019). doi: 10.1038/s41598-...

  3. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... Overview of in vitro AAV CRISPR screens. Created with BioRender.com. The in vitro option requires... screens may be AAVantageous!       In vivo, in vitro, ex vivo CRISPR screens can be carried out using...using a few different methods — in vivo, in vitro, and ex vivo. The general process of these screens is the...with transducing cells with the CRISPR library in vitro before transplanting them into mice. For in vivo...having an efficient and effective delivery method is vital for the best outcomes. The go-to in vivo option ...

  4. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...characterization of two Cpf1 orthologs that display cleavage activity in mammalian cells. Like Cas9 nucleases, Cpf1 ...see if any Cpf1 nucleases would exhibit in vivo activity in mammalian cells. From 16 diverse Cpf1 candidates... et al. found two that display robust cleavage activity similar to that of Cas9. These two nucleases, ...-base sequence. Double mismatches ablated Cpf1 activity, except when they were present in the 3’ end of... as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the 3’ end of the target...found that both orthologs show lower off-target activity than that previously observed with SpCas9. Kleinstiver...

  5. TALEN Expression Vectors

    Type
    Collection
    ...mammalian cell expression and a T7 promoter for in vitro transcription of RNA. TALENs expressed from these...

  6. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created...

  8. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created...

  12. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...APEX suffered from inconsistent activity levels and limited sensitivity under the stringently low expression... of biogenesis and mitochondrial degradation in vitro and in vivo. Merged green and red channel fluorescence...that Opto-RTKs have a similar level of background activity and activation as their wild-type counterparts...proximity-dependent proteomic mapping with far greater sensitivity at lower expression levels. APEX2 is a monomeric...system Interested in optimizing TALEN assembly and activity? The laboratory of Takashi Yamamoto has created... of both the TALE scaffold and module on TALEN activity in a single-strand annealing (SSA) assay. This...non-RVD variations”, were found to improve TALEN activity. Each positional group of module plasmids (ex....

  13. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ... website, write a blog Pursue speaking invitations by inviting others (be on a speaker committee) Follow...on the UK Career Site for Researchers called Vitae. Vitae recently published a career development framework...are always excellent Technical knowledge and creativity Become a “thought leader,” develop a technical...

  14. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid Chen Do you...

  15. AAV Viral Preps

    Type
    Collection
    ...is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy ...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...magnetically sensitive and could manipulate cell activity in vitro. The Guler lab went on to test Magneto2.0 ...(Q3) | December 2016 (Q3) MARCH 2016Enhancer activity mapping with STARR-seq   Although many technologies... identification and quantification of enhancer activity have remained challenging due to the variable ...genome-editing tools that are deployed in both in vitro cell systems and diverse model organisms. Addgene...verified in zebrafish and was shown to offer high activity and unparalleled specificity in genomic targeting...cells using the Protein A tag. The luciferase activities can then be measured and normalized to assess...interactions. Higher relative firefly luciferase activity in the Co-IP compared of a control Co-IP indicates...

  20. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...recombinases used must have high activity and specificity with low cross-reactivity, as well as low toxicity....efficiency of only 70%. To determine the cross-reactivity of these recombinases, each recombinase and reporter...excision efficiency. B2 and R showed partial cross-reactivity; however, the other recombinase/reporter pairs...proliferating Drosophila cells by estrogen-dependent activity regulation. Heidmann D, Lehner CF. Dev Genes Evol...
Showing: 101 - 120 of 581 results