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...Notebook for Bioinformatic Analyses Use Software Apps to Save Time and Track Your Experiments Additional...Find the appropriate glycerol stock within our freezers Pick the sample into the appropriate stab culture...corresponding entry in your database. Enjoy your happy lab staff. Barcoded materials make everyone's life... is almost always worth it. Because labels are applied to containers and not the mushy, gooey contents...be!) bear in mind that your barcodes are being applied to the containers, not to the contents. Tightly...contents of a tube with the tube itself. If something happens to the worm that requires a change of tube, there...

...section.   Additional Resources on the Addgene Blog Apps for Lab Life Developing Lab Management Software ...

...titles and abstracts are available online or in an app prior to the session, so bookmark which posters are...background without an introduction. If someone approaches your poster, introduce yourself and ask about... a related topic, and let them know while you appreciate the thought-provoking question, you are unable... enjoy hearing the highlights so that you can appreciate the impact of the project as a whole. In addition...

... scientists––CrispyCrunch! CrispyCrunch is a web app that helps scientists design and analyze batches ...score is less than 0.03, it stops mutating. (This happens often because the HDR inserted sequence splits ...Psychology at McMaster University in Canada. He's happy to be contributing again to science.   Acknowledgements...

...letters to be very important. On the other hand, our app/dev team rarely, if ever, sees cover letters submitted...usually yes! A good cover letter won’t hurt your application, but it can certainly help you stand out.  In...consider cover letters an important part of the application. Even within an organization, there can be variance...good cover letter!) As with any part of the job application process, it's a good idea to talk to people in...do the job as described. Let’s imagine you’re applying to a job that requires ddPCR skills. Your resume... to proactively address questions about your application. These could be logistical, like briefly explaining...  Finally, it’s a good idea to state why you’re applying to the specific company or lab. Take some time...

...mutations in genes associated with dementia, such as APP and PSEN1, in most cases the intended HDR events ...with blocking mutations, we still weren’t entirely happy with our editing outcomes and in many cases still...

...Learn about more tools for reproducibility 5 great apps for lab life Developing Lab Management Software ...sciNote. Today, every lab has its own habits and approaches to record keeping. Top priorities in most cases...Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These alarming results...familiar paper-based lab notebook is still a standard approach to record-keeping in many labs. In a way, it will...digital data generated in labs. While many labs approach the situation differently, some adopting simple...might be available as well. In most cases this applies to universities. How are you currently exchanging... Besides this being of crucial importance when applying for a patent for example, it also comes in handy...

...tools as some kind of a meta/Frankenstein app? Or perhaps there is a clear winner, is there something that...the one hand we have software tools that apply some approximate or sensible rules to determine the value... more efficient.  These insights are hard to appreciate without computational support. Indeed, rational... solutions that facilitate most experimental applications in CRISPR engineering. When compared to that...easy to fault the lack of features in a single application. The question is then, should we use all tools...2013) used a combination of both empirical and approximate methods. The details of their scoring function...and it means that there is no one-size-fits-all approach. You will have to select a tool based on your ...

...knockout approaches. In these cases, we would recommend a knockdown approach instead. In this approach, you...using one or more of the approaches outlined below. Knockout cell line approach Genetic knockout-based...Knockdown approach For cell types where knockout studies are not feasible, an alternative approach is RNA... the target of interest. This approach maps onto the orthogonal approach within the five pillars and is...knockout/ or knockdown approach should be preferred. The overexpression approach is therefore best suited... use of orthogonal approaches, cell treatments, and independent antibody approaches in different assay...target-of-interest in your assay and sample-of-interest. The approaches available broadly map onto the five pillars of...

...assume that their advisor alone applies for these competitive grant applications; however, in some cases, your...the grant application process. When my PhD advisor first asked me to write a grant application due the ...mind that universities require application submission in-house approximately 2 weeks before the RFA due date...the next round of applications. Step 5: Keep writing! Each written grant application builds upon the ... project grant application" by the NIH Still want more information on the application process? What types...writing, volunteered to take the lead on various applications throughout my graduate career, and helped receive...opportunities to convince others why a project is the best approach to a given problem, Empowers the writer, And...

...intended application - Remember, just because your antibody performs as expected in one application does ...Strategies This approach compares target expression of antibody-independent approaches to your antibody-dependent...Genetic Strategies This approach uses a gene knock-down or knock-out approach to show that an antibody...protein. This approach is ideal for validating antibodies for immunocapture applications and is amenable...pause and make sure that your antibody has been appropriately validated so that you can have confidence in...in your results. But what is appropriately validated? This post will go over some of the general considerations...strategies for antibody validation in different applications. Here, I want to highlight the general suggestions...

...allow you to approach that problem in a novel way. Finally, engineering new applications to solve problems...good quality application. Even following this advice, only about 50% of my grant applications have been ...do need to be able to research and write your application, then give it to someone else to read and / or...it). It’s easy for reviewers to spot when an application is rushed (whether the idea isn’t properly researched...researched / developed or the application is full of typos) and these are unlikely to get past the initial...someone else in the department) to read over your application and give you feedback. They’ve most likely been...alterations before submitting - handing them your application 3 days before the deadline isn’t going to be ...

...of four split AAV vector approaches. 1. Overlapping  Overview: The overlapping strategy has a region of...directed repairs (HDR). Split AAV vector approaches Split vector approaches increase the size of the gene delivered...  3. Hybrid  Overview: The hybrid approach combines the overlapping and trans-splicing methods. The AAV...has a slight advantage over the overlapping and trans-splicing approaches since there are two ways for a... genomes is the basis of several split vector approaches developed in the early 2000’s which allow researchers...is not yet known. Drawbacks:  Generally an overlapping split vector has equal or higher transgene expression... on the rate of transgene expression with an overlapping AAV since both of these variables influence whether...

...is this application - the creation of sequence-specific knockins - for which the AAV approach is most ...for a given experimental approach. First, unlike CRISPR, the AAV-based approach is only able to target ...simultaneously in the same cell, are more appropriate for a CRISPR-based approach. Second, the AAV preps generally...limitations, the AAV approach is best suited for tissue culture based approaches that require modification...researchers could apply this technique to efficiently deliver large repair templates for applications that require...working to develop high efficiency gene targeting approaches improved on this finding with the addition of...challenging to develop high efficiency CRISPR-based approaches for the generation of knockins via homologous...

.... This is the direct approach to immunoimaging assays. But if the direct approach isn’t giving you enough...   Figure 1: The direct approach (left) versus the indirect approach (right). Primary antibodies ...varies across applications. Western blots, for instance, almost always use the indirect approach, while flow...immunocytochemistry may all use either the direct approach or indirect approach, depending on the requirements of the...interest, it may be time to look at the indirect approach - which means it’s time to talk about secondary...binds to the primary antibody. In the indirect approach, the signaling molecule is conjugated to the secondary...antibodies can bind to a single primary antibody, this approach can greatly increase the sensitivity of an assay...

..." are summarized below. Check Out Erik Snapp's Full "Applying for a Faculty Position" eBook Here 1. Have...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...along with new thoughts on research statements. Snapp, who is always looking for ways to improve his eBook...suggestions, please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog...years ago, I decided to write a "how to" manual on applying for faculty positions in biomedical science. My...talents and ideas. Some key points from my ebook, "Applying for a Faculty Position: the View from Both Sides...experts in your field. Not true. Even if you are applying to a department in your specialty area, it's a...

...safe for clinical applications. Excisable Cre-lox lentiviral vectors: This approach is the same as using...of excision, this approach is still not considered safe for therapeutic applications. piggyBac (PB) transposon...This is considered a safer approach for developing therapeutic applications, but it can take a long time...time, scientists have used almost all available approaches for generating iPSCs. The generation of iPSCs...Retroviruses are not considered safe for clinical applications.     Lentiviral: Lentiviral vectors infect dividing...dilution via cell division. A drawback to this approach is it has lower levels of efficiency at generating...express reprogramming factors for therapeutic applications. Sendai viral vectors: Sendai virus is a single...

...relationships. However, most job seekers will and should apply for jobs posted online as one part of their job ...Utilizing existing relationships as part of the job application process can dramatically increase the chances... of a resume can be crucial for a successful application. It takes time to prepare an excellent resume... Many job postings will result in hundreds of applicants the day they are posted.  If your resume is not... here for more on timely submission of a job application. 3) Read job descriptions carefully Be open minded...have to have all of the listed qualifications to apply for a position.  A job description is usually a ...wish list and the hiring manager knows that no applicant is likely to meet all the criteria. Note the criteria...

...skipping approach in mice, DMD researchers are very excited. This approach may also be applicable to a number...success in basic research, researchers are eager to apply it in a clinical setting. CRISPR is often used for...), an X-linked recessive disorder affecting approximately 1 in 5000 males. DMD is caused primarily by ...very large (79 exons), but much of the sequence appears to be nonessential. Within the mutational hotspot...so researchers have set their sights on another approach. Since shorter forms of dystrophin can still be...researchers have begun to explore genome editing approaches for exon skipping. Addgene depositor Charles ... removal rate of exon 51, which resulted in appropriately localized dystrophin. In a subsequent study,...

...genes provided by the user. This approach is useful for several applications as will be discussed below. The... screen approach is to use smaller libraries targeting subsets of genes. With such an approach, you lose...custom CRISPR screen and the appropriate phenotypic readout, similar mapping could be rapidly performed ...challenges with a custom screen approach is to identify an appropriate list of genes to target. Thus, ... involved in a studied cellular behavior. This approach is particularly suitable for in vivo screens, ...drugs. Known drug targets  Another interesting approach is to design screens against known druggable targets...preferentially those for which validated inhibitors or even approved drugs already exist. If you are lucky and find...