Skip to main content

We narrowed to 317 results for: bon;

Showing: 101 - 120 of 317 results
  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...activity and instead become trapped in a covalent bond with its substrate. Then, all it takes is a bit ...find self-labeling fusion proteins or cloning backbones to study your gene of interest with these tools...
  2. Deep Dive: qPCR

    Type
    Blog Post
    ...for the target:probe/primer bonds to be more stable than the hairpin bond.  Alternatively, chemistries...
  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...domain that promotes the formation of an isopeptide bond between 2 peptide tags, SpyTag and KTag. The group...plasmids for a given species are based on the same backbone to permit expression levels to be directly compared...
  4. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...Often, the size of the plasmid insert and vector backbone are known and thus this technique can be quickly...buffer Verifying Total Plasmid Size -OR- Insert and Backbone Size The simplest form of diagnostic digest is...the expected size, or that it is composed of a backbone and insert of expected sizes. This is frequently...in two bands; the 1200bp insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting...know that you have a 1,200bp insert in a 5,000bp backbone, but there are many plasmids out there that, when...very unique pattern that will distinguish one 5kb backbone with a 1.2kb insert from all others. When choosing...orientation the insert is ligated into the vector backbone and therefore the only thing left to do is identify...
  5. Lentiviral Prep Service

    Type
    Collection
    ...Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 ...Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs ...library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs...genes and containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting...VP64) Set A Human CRISPR inhibition library in backbone pXPR_050 inhibiting 18,901 genes and containing...
  6. Summer Fun at Addgene!

    Type
    Blog Post
    ... over to our YouTube channel and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted...
  7. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed right into the box. Which is...
  8. Zhang Lab CRISPR Page

    Type
    Collection
    ...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...LacZ, plus Cre recombinase 60229 : sgRNA cloning backbone with Cre recombinase 60230 : sgRNA targeted to...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described ...control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid ...
  9. Editor's Choice, September 2016

    Type
    Blog Post
    ...opportunity to dress up as Mr. Incredible, an added bonus. You can find the rest of our September posts in...
  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ...BMP5 bone morphogenetic protein 5 MGC34244 BMP6 bone morphogenetic protein 6 VGR, VGR1 BMP7 bone morphogenetic... MGC34632 BMP1 bone morphogenetic protein 1 FLJ44432, PCOLC, PCP, TLD, pCP-2 BMP10 bone morphogenetic ...protein 10 MGC126783 BMP15 bone morphogenetic protein 15 GDF9B, ODG2, POF4 BMP2 bone morphogenetic protein ...protein 2 BMP2A BMP3 bone morphogenetic protein 3 BMP-3A BMP4 bone morphogenetic protein 4 BMP2B, BMP2B1, MCOPS6...protein 7 OP-1 BMP8A bone morphogenetic protein 8a FLJ14351, FLJ45264 BMP8B bone morphogenetic protein...BMPR1A bone morphogenetic protein receptor, type IA 10q23del, ACVRLK3, ALK3, CD292, SKR5 BMPR1B bone morphogenetic...
  11. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...digested vector backbone. This is accomplished by covalently connecting the sugar backbone of the two DNA..., the insert DNA is physically attached to the backbone and the complete plasmid can be transformed into..., it is important to treat the digested vector backbone with a phosphatase before performing the ligation...
Showing: 101 - 120 of 317 results