We narrowed to 324 results for: cloning

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).

Tris? Tris-EDTA? EB? There's many options for eluting plasmid preps. Find out how elution and storage temperature affects DNA stability and downstream applications.

Addgene is pleased to announce the release of the 4th edition of ours Plasmids 101 eBook!

Plasmid-encoded antibodies offers many advantages over other types of antibodies. Learn about how recombinant antibodies are made.

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

Announcing Addgene's new Intro to the Lab Bench, a collection of protocols, videos, and quizzes to help guide those new to working in the lab.

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

Learn about the components of a plasmid page on our website and shows how the different components can be useful to you

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Learn about some things to consider when designing your fusions - where to make the fusions, the size and shape of the protein, and linker size.

Having trouble with viral vectors? Here are several tips to help prevent recombination in viral vectors or other plasmids with repeating elements.

Scientists from UCSF identify 332 human proteins that the SARS-CoV-2 virus interacts with in cells in attempts to find druggable targets.

In this blog post, we explain gamma-retrovirus packaging systems for those interested in using gamma-retroviral systems in the lab.

Learn about new plasmids at Addgene including AAV split base editors, Ace2-mScarlet voltage indicator, CyanoGate MoClo cloning toolkit, and CreLite.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

Recent highlights from Addgene's collection include tools for epigenetic engineering, tracking cell-cell interactions, and a new auxin inducible degron system for C. elegans.

If you can't find a validated antibody for your flow experiment, this blog post can help you validate your antibodies yourself.

Check out all of the plasmids featured in our hot plasmids articles from 2017.