We narrowed to 324 results for: cloning

...plasmids uses the same restriction enzymes for cloning (see schematic below), enabling a researcher to...tags Resources on Addgene.org Find Additional Cloning Kits ...

...were used in this study.  The zCas9i cloning kit is a plant cloning kit that contains a series of vectors...CRISPR-based lineage tracing tool captures rare clones that represent 0.001% of the population.  Because...

...terminus the tag will end up on after successful cloning. Cloning information: This section provides useful ...plasmid's structural features and see if I can easily clone my gene into it? Your best bet is the Plasmid ...

...without a kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts Resources on...Molecular Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...

...complex eukaryotic cells, but would prefer to grow, clone, and manipulate their vectors in fast-growing bacteria...example, if you are using E. coli simply for easy cloning and quick DNA production, you probably don’t need...called transfer vectors. The gene of interest is cloned into a shuttle vector for transfection into producer...

... available, so you’ll only have to worry about cloning your transfer plasmid. The transfer plasmids have... be produced in insect cells, but they require cloning specific AAV or lentiviral machinery into a baculovirus...bacmids are readily available, so the amount of cloning required is about the same between insect and mammalian...

...plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning Read..., as detected by qPCR, but this did not reduce cloning efficiencies.  When compared to fresh columns, ...

...from plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The fourth...

...co-transform pBLADEONLY. That way, a previously cloned gene of interest, under the pBAD promoter, can ... controlled with light without the need of any cloning.     What advantages does BLADE have over AraC...

...in your cloning experiments and tired of having to PCR many colonies to find a successful clone? Addgenie...

...ready-to-use viral preps, pooled libraries, kits, and cloning grade DNA!     This is just the first of many ...

...directly transfect cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning applications... analyses are performed on my cloning grade DNA order? For each cloning grade DNA prep, the plasmid sequence...like to see available as cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form... ordering cloning grade DNA for over 200 of our most popular plasmids! DNA... DNA Service: Cloning Grade DNA ...Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over 200 plasmids in our ...variety of popular plasmids and backbones with high cloning potential. By making these plasmids available as...

...ligands for different experiments without having to clone a whole new construct for every application. Plus...your labels easy! Instead of taking the time to clone a new construct for every new-and-improved fluorescent...able to get a plasmid ready-to-use without any cloning at all! As mentioned above, several variants of...collection to find self-labeling fusion proteins or cloning backbones to study your gene of interest with these...

...to COVID-19. We had many protocols for plasmid cloning and working with viral vectors, but what we lacked...

...samples. For a more detailed comparison of these cloning strains, browse the table of common gene mutations...N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia...

...knock-in at a genomic locus or more commonly via cloning of recombinant proteins. Why use a tag? If you...developed in 1985 and is still the most prevalent clone used today! Goat, chicken, mouse, or rabbit? Addgene...

...as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Synthetic... vectors. ID Kit Description Cloning Type PI 1000000036 GreenGate Cloning System Plasmids for rapidly ...Stuttmann 1000000233 Joint Modular Cloning (JMC) Toolkit Modular cloning chassis and set of functional parts...Explore plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene ...Collections Plant Plasmids Plant Plasmids and Resources Cloning & SynBio Genome Editing Gene Silencing Reporters... general and species-specific gene expression. Cloning and Synthetic Biology Expression Vectors The table...Kit Plant synthetic biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9...

...related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems....Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning and Genomic Tools...Tools Synthetic Biology: Cloning and Genomic Tools SynBio Resources SynBio Home Browse All SynBio Assembly...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination...Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table by keyword or sort by...

...) (Latynski and Valentovich 2014). We routinely clone the purified amplicons upstream and downstream of...transcriptional readthrough into the MCS to facilitate the cloning of toxic products. To allow manipulation of naturally... they are just spontaneous antibiotic resistant clones. But if they are blue or magenta, they are real...

...protein. These were either (i) coded by the multiple cloning site and therefore consist of random amino acid... often use restriction enzymes in the multiple cloning site for generating fusions. For the POI-fluorescent...PVAT, when we use the AgeI site in the multiple cloning site) is sufficient. For the fluorescent protein-POI...