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We narrowed to 302 results for: human dat

Showing: 101 - 120 of 302 results

  1. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...produced by scientists - not robots. Unfortunately, humans have been known to make mistakes. What if a tube... of accidental errors increases. In addition to human error, fragments of DNA from the helper plasmids...Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089...Updated Jun 1, 2021 by Meghan Rego. Reproducible data are key to science, so scientists are used to repeating...DNase-treated AAV and submitted for NGS. Raw sequencing data is analyzed to determine the identity and serotype...BLASTed against the NCBI nucleotide collection database, and it may also be aligned to plasmids in our...contaminant is Addgene plasmid #44362. Method validation, limitations, and future plans How did AAV-68544...

  2. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...that CRISPR-Casɸ is active in vitro as well as in human and plant cells. Casɸ from Biggiephage can...lentiviral CRISPR knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy.... CRISPR screens by using this lentiviral CRISPR human genome-wide library deposited by Leopold Parts. ...the most photostable yellow fluorescent protein to date By Justin Ng High-throughput screening methods, ...the parent YFP, and is the most photostable YFP to date. As such, mGold is expected to contribute to experiments...

  3. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ..."Genome-scale CRISPR-Cas9 knockout screening in human cells." Science 343.6166 (2014): 84-87. PubMed PMID...PMC4089965. 9. Wang, Tim, et al. "Genetic screens in human cells using the CRISPR-Cas9 system." Science 343.6166...Massively parallel high-order combinatorial genetics in human cells." Nature biotechnology (2015). PubMed PMID...barcodes. CombiGEM is highly flexible and can accommodate any genetic elements of interest. It can thus...

  4. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...The same way the human body is made up of organs, cells comprise compartments and structures, called ...sensitive to detecting light signals not visible to the human eye and are indifferent to subjective color intensity...More about Plasmids for Endogenous Gene Tagging in Human Cells Check Out the Michael Davidson Fluorescent...created your fusion protein, it's important to validate its function relative to the untagged, wild-type...wild-type protein. You may, for example, validate that the fusion protein localizes properly by comparing the...protein of interest. Scientists are continuously updating and optimizing FP tools to avoid these problems...

  5. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...provide CRISPR libraries targeting the mouse and human kinomes. While any subset of the genome is limited...individual lentiGuide gRNA plasmids targeting the human kinome. These plasmids contain the same 3-4 gRNAs...Our New and Improved Pooled Library Page Find Validated gRNAs Deposit Your CRISPR Plasmids ...

  6. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...donor DNA leads to a vast majority of AdV-modified human cells being subjected to scarless homology-directed...induces targeted mutagenesis in a diverse array of human cells. Sci Rep 4: . https://doi.org/10.1038/srep05105...Liver in Spite of Cas9-Specific Immune Responses. Human Gene Therapy 26:432–442 . https://doi.org/10.1089...Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel.   Adenoviral vectors... 2012). These features make AdV an attractive candidate for in vivo delivery of advanced genome editing...

  7. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ... published in 2013, revealing that about 70% of human genes have a counterpart in zebrafish. Understanding... genes in fish will help clarify their roles in humans. The sequenced genome also permits “whole-genome...successful disease models, and are used to study human illnesses such as blood disorders, diabetes, cancer...fertility. Several zebrafish-specific gene expression databases that provide information about when and where ...developed to help parse and visualize expression data in useful ways. Zebrafish mutants In the 1990’s,...China. In addition, the Zebrafish Model Organism Database ZFIN actively curates information on mutants and...types. Sequencing-based lineage tracing can help elucidate the gene networks that transform zygotes into ...

  8. The Challenges of Cell Culture

    Type
    Blog Post
    ...contaminated with HeLa cells, the first immortalized human cell line. He proved this by examining whether the...the tens or hundreds of millions, let alone the human cost. Here are just a few publications which have...can place suspects at the scene of a crime (every human in the world has a unique STR profile) and can also...cell lines on public databases. Image attributions (from top to bottom) Database Center for Life Science... Cell Line Authentication Committee includes a database listing all cross contaminated or misidentified...colleague?”. The main difference is the QC and validation carried out by the commercial repository. Repositories...incubator containing the passage number, cell line, and date, which you complete each time you passage the cells...

  9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...HSPG)3 AAV receptor (not strictly required)2 Human fibroblast growth factor receptor 1 (FGFR1)3 αVβ5...AAV1, AAV6, AAV7, and AAV8 in the Mouse and Rat. Human Gene Therapy, 19(12), 1359–1368. https://doi.org...After Intravenous and Direct Kidney Injections. Human Gene Therapy, 30(12), 1559–1571. https://doi.org...-associated virus serotypes: Vector toolkit for human gene therapy. Molecular Therapy: The Journal of ...administration doses. Don't forget to submit your validation data after your experiment! Thanks to Addgenies ...species, so be sure to check the literature and validate your choice of AAV vector for your experimental...system. As we cannot make universal serotype recommendations, the following table focuses on tissue tropism...

  10. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...my target sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary...nucleotide to the start of my target sequence? The human U6 promoter prefers a 'G' at the transcription start...IDT (non-PAGE purified oligo is fine based on new data). For general transfection, you could use 1ul of..., Science 2013 ) do you have any suggestions? To date, I have never used the Ex Taq. In our hands, Herculase...

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...becoming available. At the same time advances in human genetics led to the identification of several disease-causing...visual impairment that faithfully recapitulated the human condition. Luck brought all these key elements together...therapy. It is not known to cause any disease in humans, and its low immunogenicity is critical when systemic...results in preclinical studies some potential candidates for rare to ultra-rare ocular diseases are being... co-founded a non-profit to help push forward candidate therapies for these diseases. Riding on the early...

  12. The Strength of Story Telling

    Type
    Blog Post
    ... Editor of Naturejobs, part of Nature magazine. Humans are built to enjoy, remember, and listen to narratives... asking someone how and why they left academia. Humans are designed to pay attention and enjoy anecdotal...around the self, too — when talking to friends or dates or colleagues we tell tales about parts of our lives...email address far and wide, keep your website up to date, invite people to ask questions, be approachable...

  13. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...proteins is essential for human health. Failure to do so is implicated in several human diseases. Proper patterning..., etc., again in clear terms. "Implications for human disease" is vague. Instead, consider "A mechanistic... your candidacy. A major reason that a lot of candidates either get no interviews, or get several interviews...accomplish. No matter how many times I say this, candidates regularly try to convince me that their search...Nothing turns off an interviewer faster than when a candidate says "I'm not an expert in [your research area...with feedback from their own job searches. I've updated chapters to reflect new technologies, i.e. video...I'll try to answer your question and subsequently update the ebook. One of my graduate students encouraged...

  14. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...description of the VGS process and use cases in Human Gene Therapy. Existing methods for AAV quality control...Adeno-Associated Viral Genomes in Academic Settings. Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089/...the sequencing data from multiple AAV lots can be run in parallel and provide serotype data in minutes and...service in 2016, we adapted this platform to accommodate adeno-associated viral vector (AAV) samples and...open-source custom Python script that uses the VGS data to determine the percent of recombination present...pre-defined serotype specific signature sequences in the data. Unlike previously described thermodynamics-based...Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated ...

  15. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... use in revealing antigenic differences between human, canine and simian isolates. J Gen Virol., 68 (Pt...Mycobacterium abscessus, an increasingly common human pathogen notorious for its high resistance to antibiotics...luciferase is embedded in the I10 domain of the human titin protein and only exposed when the module unfolds...from Addgene!   Figure 1: Experimental validation for Anti-V5 [IPI-SV5-Pk1] in a western blot assay...design and studies of protein ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova...

  16. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...CRISPR knockout screens, the Bassik lab created a human knockout library that has 10 gRNAs per gene and ...on-target activity in high-throughput screens. The human and mouse versions of the Bassik CRISPR Knockout...genomic perturbation screens: gold standards for human functional genomics." Molecular systems biology ...infected with Cas9. Validating the Library The CRISPR knockout library was validated via a growth screen...

  17. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...economic and cultural importance of these animals to human populations around the world. Here at Addgene, we...revisit some of the ways these animals have impacted humans through biomedical research. The Camelid family...radioactively labeled sdAbs have become appealing candidates for the detection of different cancers (Jin, ...

  18. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...have emerged as a powerful model for understanding human physiology, development, and disease. In order to... to morphogenesis, cell fate specification, and human disease.     Sun, et al. bioRxiv. 2021. https:/...plants, this approach was also used to build and validate a ratiometric fluorescent biosensor based on the...

  19. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...neurotransmission. But with approximately 950 different human genes encoding GPCRs (Takeda S et al., 2002) and...Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https...donor and a fluorescent acceptor. TRUPATH uses an updated version of BRET called BRET2. Figure 1: TRUPATH...

  20. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...my target sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary...nucleotide to the start of my target sequence? A2: The human U6 promoter prefers a 'G' at the transcription start...Systems has expanded out of control. To further consolidate these FAQs, Le Cong worked with Addgene Senior...
Showing: 101 - 120 of 302 results