...encompasses a variety of technologies to functionally investigate the human genome. Doench says he enjoys working...with RNAi in terms of determining what genes are involved in biological process X, you can do with CRISPR... of individual edits can be studied quickly. Involvement with Addgene Although Doench’s thorough understanding...understanding of CRISPR is what got him involved with Addgene - he says he’s “known of Addgene basically...

...solving technical problems in the lab, management involves a different skill set: mentoring, setting strategic...industry, but the transition wasn’t easy - I had to reinvent myself and redefine the value I could bring to...a solid foundation in the basics of management. Invest time in understanding key principles through books...and project management. Mentorship is another invaluable resource: connecting with mentors who have made...

...viral production and distribution, this project involved every Addgenie in the company. Our Finance, Business...continues to make improvements to our website and inventory management systems to make way in our systems ...Maintaining our excellent service and accuracy involves caring and careful Addgenies, but also a lot of...Software and IT Teams made major upgrades to our inventory management systems to enable efficient, tracked...

...post, we invite you behind the scenes in our content writing process, and to even get involved yourself...standards. It’s always a work in progress! Get involved A lot of our content already comes from you and...depositors and customers are asking. So how can you get involved? Keep asking your burning questions and tell us...

...to do that we’ve had to navigate the challenges involved in plant synthetic biology. Challenge #1: Public...unique metabolic processes of plants are being investigated on limited funding and time. While institutions... standing behind it. Our color changing flower involves two basic components: the pigments (anthocyanins...research for decades, and the biochemical pathways involved in central anthocyanin metabolism are well understood...

...using the kit to target a large number of genes involved in cardiovascular and metabolic diseases. We are...Addgene: Tell me about the history. How had investigators done this kind of work before? Musunuru: In ...Unfortunately, it is time consuming and difficult for investigators without a lot of expertise to create zinc finger...and puts it in the hands of pretty much all investigators. Our kit makes it extremely simple to design...

... of genes involved in the execution of a specific regulatory regime (such as genes involved in metabolism...unknown. Together, NRs govern expression of genes involved in a broad range of reproductive, developmental...‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the more loosely bound...

...networking with your local education community. Get involved in education advocacy non-profits like Phipps ...science projects Lastly, if you'd like to directly involve the public in your research projects, you can set...genomes requires lots of data. Get your community involved in sampling or bioinformatics analysis or upload...project. Science communication opens doors Getting involved in science communication has been the most rewarding...

...Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’s experience... is now studying how Argonaute pathways may be involved in preventing silencing of self-genes. Dr. Mello...presented a hypothesis that these pathways are involved in RNA based surveillance of repetitive gene families... natural role in prokaryotic immunity to viral invasion.  His lab is studying P. furiosus, a hyperthermophile...

...public to access basic equipment and to become involved in developing tools and solutions to research ...makerspace is. The computer scientists can get involved in modelling, the electronics folks can build ... top of that model, and the biologists can get involved a in variety of aspects from the molecular biology... feel free to contact your local space and get involved! Derek Jacoby is a PhD student at the University...

...less work for the first two steps, as no mice are involved. Ex vivo starts with transducing cells with the...lentivirus requires a BSL-2 setup. This requires a less involved setup when working with mice and slightly less...high degree. This is particularly helpful when investigating tissue-specific diseases, as you can target ...like Cas12a (Cpf1). Even more creative options involve generating strains of mice that stably express ...

..., but surprisingly, it is considered minimally invasive. Restraint: anesthesia Location: spinal cord Gauge...intubation, this procedure is considered quite invasive. Restraint: anesthesia Location: trachea Gauge...differences are that intranasal is easier, less invasive, and only requires a pipette. Since the substances...relief, straight to the stomach. This technique involves administering medication through a feeding tube...

...Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications...applications. But how does one invent a tool for applications that don’t even exist yet? Andrew York, Maria...applications are still yet to be demonstrated (or invented), its behavior has already been successfully reproduced... chemistry and protein biophysics, as well as inventors and biomedical engineers, join efforts to explore...

...step and mix the samples well by vortexing or inversion. Created with BioRender.com.   When choosing...curve, change pipette tips and mix thoroughly by inversion or vortexing between each step of the series. ... dynamic range of the curve, then the assay is invalid. If the unknown sample’s measurement is too low...increase the accuracy of the data. For assays involving sigmoidal curves, manufacturers often recommend...

...programmability: depending on the transposases involved, they insert either randomly, or at fixed genomic... L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon system. (B) ... a streamlined expression approach (Fig. 2A), involving two-plasmid or single-plasmid systems (Vo et al...insertions are not currently possible, unlike methods involving homologous recombination. Additionally, these ...

...researching, winning, and effectively managing grants involves much more than writing. In fact, many grant applications...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...organization in a position that required some involvement in the grants process and managing programs that...

...months, in fact. In the Lee-Parsons lab, we are investigating how the production of cancer therapeutic drugs...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...transient methods are usually the first choice for investigating gene function, as these experiments can be done...species This will save you a lot of time. Don't re-invent the wheel. Test different leaf ages Usually younger...

...experimental hurdles I would encounter. This was invaluable to me as a bench scientist working at an institution...available. Even more valuable, however, was when she invited me to lunch for a “tweet-up” with other folks at...not participate but provided moral support was invaluable.  Mixing metaphors and scientists - Culturing...participants feel like their mentors weren’t as invested in how their meetings went. While this was expressed...

...high-quality maps and annotations without us having to invest too much of our internal software development resources...zone As part of my position at Addgene I am now involved in Strategic Partnership Management, a very interesting...for discussions and potential collaborations. We invited experts from other universities and maintained ...ways to maintain a relationship - many of them involve personal meetings and common interest so naturally...

...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...behaving properly, FPs enable researchers to investigate the localization and dynamics of fluorescent ...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...overestimation of fusion protein activity. We encourage investigators to characterize FP-fusion proteins functionally...