We narrowed to 167 results for: ipp

1. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   ...lentiviral in nature, so please ensure that you are equipped and authorized to make and use lentivirus. Also...

2. Plasmids 101: The protein expression toolbox

   Type

   Blog Post

   ...domains (DD) are akin to degron tags, just with a flipped mechanism. When a protein is tagged with a DD, ...

3. RNA Extraction Without A Kit

   Type

   Blog Post

   ... mid 1980’s set out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski...

4. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   ...feel free to make the decision by price point, shipping time, or other factors. Just remember that these...

5. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   ...help convince a future employer that you are well equipped to work through challenges from day one in your...

6. dTAG - You're it!

   Type

   Blog Post

   ...co-opt E3 ligases such as cereblon (CRBN) or von Hippel-Lindau (VHL) to bring the endogenous degradation...

7. Addgene: The First Twenty Years

   Type

   Blog Post

   ... our repository grew to over 150,000 plasmids, shipped out our two millionth item, and had a cumulative...

8. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   ...stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible with most...

9. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   ...dynamics of newly synthesized proteins in rat hippocampal neurons." Nature neuroscience 13.7 (2010): 897...

10. Human Germline Editing Using CRISPR

    Type

    Blog Post

    ...always come with some uncertainty. As biologists equipped to understand the results of this paper, we should...

11. Developing Lab Management Software for Biology

    Type

    Blog Post

    ...technicians to retrieve the sample and get it ready for shipping. In the first case, we naively assume that everything...

12. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    ...work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is...

13. Hot Plasmids - October 2020

    Type

    Blog Post

    ... tracking the synaptic maturation pattern of hippocampal adult-born granule cells over time that were ...

14. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    ... as showing a very bright signal only in the hippocampus compared to other regions, that informs us that...

15. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    ...accessible genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary cells and...

16. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    ...personalized medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes...

17. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    ...immediate effects of specific neuron firing are better equipped using fast-acting optogenetic tools. The advantages...

18. Design Tips for Prime Editing

    Type

    Blog Post

    ...-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson...

19. Plasmids 101: Modular Cloning

    Type

    Blog Post

    ...piece. Rob is postdoc in Peter Schultz’s group at Scripps Research, where he works on genetic code expansion...

20. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    ...to divide. However, if fueled with caffeine and equipped with the pDCAF3 operon, the growth of this knockout...