We narrowed to 187 results for: lentiviral
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TypeBlog Post...comprehensive transcription factor library, in lentiviral vectors. At 3500 plasmids, this represents the...
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22 Hot Plasmid Technologies from 2014
TypeBlog Post...., BMC Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems...Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...CoChR have been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants...purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids from ...upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression... Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...Mammalian Expression pLenti6.2_miRFP670 - Mammalian Lentiviral Expression pBAD/His-miRFP670 - Bacterial Expression... -
Supporting Rare Disease Research with BeHeard 2017
TypeBlog Post...mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown,... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used plasmids made... -
Adenovirus Plasmids
TypeCollection...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...single-cell level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable...reprogramming and differentiation Check out our lentivirus guide Sign up to receive our Viral Vectors 101... -
Meet the New Addgene Board Members!
TypeBlog Post...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...specific DNA sequence in only 1 min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...basic mammalian expression system and go for a lentiviral or a retroviral backbone. This is considered ... -
CRISPR Guide
TypeCollection... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post...performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR...stably expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1)....Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production...of A549 cells transduced with Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...deaminases like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and ... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...vector background, so you can package them into lentiviral vectors if you need to. Additionally, they have... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...Development of a novel mouse glioma model using lentiviral vectors." Nature medicine 15.1 (2009): 110-116... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...high-throughput manner. They designed the pXPR_053 lentiviral expression vector, which contains an sgRNA insert... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...proven useful to the study of SARS-CoV-2 are the lentiviral production and packaging vectors VSV-G and psPAX2... -
Plasmids 101: Screens vs. Selections
TypeBlog Post...CRISPR libraries Genome-wide screens with CRISPR Lentiviral CRISPR libraries ...