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We narrowed to 153 results for: lentiviral vector plasmid

Showing: 101 - 120 of 153 results

  1. Reagent Repositories Are Speeding up Science During the Pandemic

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    Blog Post
    ... study of SARS-CoV-2 are the lentiviral production and packaging vectors VSV-G and psPAX2, which were ...these plasmids from Addgene. This publication then resulted in the development of a new plasmid, pCMV14...continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research...work to share plasmids to all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse...Biological repositories that distribute mouse strains, plasmids, cell lines, and much more have been critical ...science is truly a global endeavor. Case study 2: Plasmids Another example of reagents that have broad applications...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, 2020 to ...

  2. Lentiviral Prep Service

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    ...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled ...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in our repository...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository ...

  4. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post
    ...women with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene, the lab will express these ... with the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...highlight their research and how they will use plasmids and viral preps from Addgene to further their ...BeHeard award, the ACD will use Addgene’s RESOLUTE plasmid collection to perform a functional screen in mammalian...therapeutic development,” says Iyer. Since the RESOLUTE plasmid collection consists of human and murine solute ...contraction defects. The lab also plans to use CRISPR plasmids to generate deletions of candidate genes in euploid...research questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building...

  5. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer and ...kit has a focus on infrastructure with shuttle vectors, several delivery systems which can be engineered...engineered into the TUs, and even viral gene silencing vectors. The Plant Parts III kit has a focus on transformation...between CRISPR components expressed from different plasmid backbones. They noted that backbone differences... as the order, or distance in the placement of plasmid elements can impact expression levels and consequently...plants, and animals (Chiasson et al., 2019). Many plasmid elements such as promoters or fluorophores are ...Gantner, Johannes, et al. “Peripheral Infrastructure Vectors and an Extended Set of Plant Parts for the Modular...

  6. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...comparison of an shRNA plasmid sequence and an miRNA plasmid sequence. (A) Plasmid sequence from pLVUHshp53...Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA machinery....   Expressing shRNAs using plasmids The design of shRNA plasmids requires three important steps: ...between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence...shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is meant...with Cas13a (C2c2) Plasmids 101: The Promoter Region – Let's Go! Browse All Plasmids 101 Posts Additional... occur via transfection or viral delivery of a plasmid. RNAi is an umbrella term for a number of different...

  7. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post
    ...Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources ...our Stem Cell Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab ...include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid electroporation (episomal... [7, 8] and mRNA transfection [9]. Many of the plasmids used for these methods can be found on Addgene...Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...and in vitro methods are similar except that the vectors carrying the transdifferentiation factors are directly... (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234...

  8. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ...-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...

  9. Plan Your Experiment

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    ...viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral vectors... in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. ...'s collection of CRISPR plasmids . Delivery Using Viral Vectors Viral vectors are a great option, as they...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...

  10. Making CRISPR Plasmids Using Fragmid

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    Blog Post
    ...modular vector assembly process designed for a quick, plug-and-play approach to cloning CRISPR vectors (McGee...for each module, generate the vector file, and quickly clone their vector of interest. And it truly is ...destination vectors for various delivery methods, along with compatible inserts. All destination vectors contain...heavy lifting out of CRISPR vector design, making it easier to get the vectors that are just right for your...restriction digest, and then plasmid sequencing if you can. To quote Doench, “Whole plasmid sequencing is just ...correctly banded clones for plasmid sequencing and found 98% of the plasmids were perfectly correct. Because...least one plasmid was the correct sequence for every individual assembly. What destination vectors are available...

  11. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post
    ...millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family...Genome Edit Resources on Addgene.org Find CRISPR Plasmids for Your Research Check out Our CRISPR Guide Find...family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...

  12. Zhang Lab CRISPR Page

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    ...Addgene plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422...VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper...guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual vector system uses S. pyogenes Cas9...SpCas9 plasmid series. ), and a pair of annealed oligos can be cloned scarlessly into the vector before...

  13. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post
    ...katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line. This...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...Guide or browse our collections of optogenetics plasmids – and keep an eye out for future updates to the...Additional resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org...Addgene.org Optogenetics Guide Optogenetics Plasmids References Aljiboury, A. A., Ingram, E., Krishnan, N...

  14. Fluorescent Protein Guide: Empty Backbones

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    ... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+...Species) Organism/Expression System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP...Verkhusha Lab Plasmids - Enhanced colors, including PSmOrange, iRFP Gradia Lab Mammalian Plasmids - Includes...Lab Plasmids - Includes many N- and C-terminal fluorescent proteins Insect Sutherland Lab Plasmids - For...expression Davidson Lab Plasmids - Includes many fluorescent proteins in the pBAD vector Jump to Top References...

  15. Viral Vectors 101: Virus Safety

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    Blog Post
    ...transfer plasmid), the envelope (Env) gene on a separate plasmid (envelope plasmid), and the polymerase ...different plasmids. For a retroviral system this looks like the genetic cargo in one plasmid (the transfer...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection...Viral Plasmids Which Type of Virus Should I Use? Resources on the Addgene blog Viral Vectors 101: ... transfection of all three plasmids into a producer cell line, such as HEK-293T, production of viral particles... and Pol genes are not encoded in the transfer plasmid, the component that physically integrates into ... retroviral packaging components onto distinct plasmids for safety.    Viral cargo-specific safety...

  16. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    ...separate plasmids and expressed via cells cultured with the vectors. This allows the viral vectors to reproduce...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that you know what viral vectors are, it...viral particle is called a virion. Finally, viral vectors, which are used in the lab, are distinct from viruses...

  17. Viral Vectors 101: Transductions

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    Blog Post
    ...Production at Addgene Viral Vectors 101: What is a Virus? Viral Vectors 101: Viral Vector Elements ..., a viral vector is selected to perform the transduction with: either retrovirus, lentivirus, adenovirus...desired DNA to be integrated can be cloned into the vector that you have selected. The nucleic acid will be...produced, and viral particles are packaged, when the vector is expressed. Once the design and construction ... it’s time to make virus! The constructed viral vector containing the cargo of interest is then typically...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK-293...-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...

  18. AAV Vector Quality Control: Going the Extra Mile with NGS

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    ...DNA from the helper plasmids or the cell genome can be packaged inside the vectors during production. ...cell genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al....blog Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We...Ready-to-Use Lentivirus Browse Viral Plasmids ... of poor reagent quality. To make sure our AAV vectors are of the highest quality, we undertake a rigorous...To make sure that adeno-associated viral (AAV) vectors available at Addgene are suitable for in vivo studies...should you care about viral quality control? AAV vectors are produced by scientists - not robots. Unfortunately...

  19. Viral Prep Service for Plasmids and Pooled Libraries

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    ...technologies. Penn Vector Core AAV Vectors Many AAV vectors from the Penn Vector Core have been transferred to Addgene...Viral Vector Packaging Service Addgene produces high-quality viral vector preps from select plasmids in ...eligible AAV plasmids and we’ll make the viral vector prep you need. Packaged on Request Viral Vector Packaging.... We know that viral vector production is a common step after requesting plasmids from Addgene, and by...In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer preps...about viral vector production at Addgene, ordering information, and more. Additional Viral Vector Resources...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...

  20. Viral Vectors 101: Viral Applications

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    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...We offer a variety of viral vectors, viral services and even a Viral Vectors eBook to get you started. ... terrains. They are, in fact, a choice delivery vector from basic science research all the way to clinical...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily... long-term, and even inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been...interest, turning expression off permanently. Viral vectors can also be used to express proteins. Viral expression...of interest, and often a selectable marker. This vector can express your construct in target cells transiently...
Showing: 101 - 120 of 153 results