We narrowed to 136 results for: max

1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   ...with minimal side-effects on healthy cells and maximal effect on unhealthy cells. Until recently, the ...

2. Negotiating Work and Life: How to Find the Joy

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   ...negotiating our energy and attention. The goal is to maximise the time you are enjoying both work and personal...

3. Hot Plasmids Spring 2024

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   ...in some cases, over 20-fold higher editing than PEmax. The authors hypothesized that the improved efficiency...

4. Hot Plasmids - November 2023

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   ...large edits with similar or better efficiency than PEmax, the current state-of-the-art. PE6b was very efficient...

5. CRISPR 101: Any Base Transversion Editors

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   ...from abasic sites. This approach resulted in a maximum A → T product purity of 66%. Pro tip! Abasic sites...

6. AAV Titers: Where Do They Come From And What Do They Mean?

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   ...contains one viral genome. Other than a theoretical maximum value, physical titers don’t necessarily give any...

7. The Challenges of Cell Culture

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   ... been used up. This ensures you can retain the maximum stocks and use of the original Cell line you purchased...

8. Plasmids 101: Control Plasmids

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   ...a gene, you should design a control that shows maximal activation. Likewise if you are trying to repress...

9. Tips for Using FRET in Your Experiments

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   ...choosing a complementary pair of fluorophores. To maximize the FRET signal you should choose the highest ...

10. March for Science

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    ...Science Part: Democratic values and their role in maximizing the objectivity of science.  Scientific American...

11. Fluorescent Proteins 101: When GFP lets you down

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    ...1998). The deprotonated state has an absorbance maximum around 488 nm and it emits light that peaks at ...

12. Quick Guide to Near-Infrared Fluorescent Proteins

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    ...The numbers in (e)miRFP names correspond to the maxima of their emission spectra. These probes can be ...

13. PiggyBac-ing Through the Genome Editing Field

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    ...Importantly, by varying these ratios, you can either maximize or minimize expression levels of your gene of ...

14. Plasmids 101: Restriction Cloning

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    ...quantities of DNA, you’ll need to do a midiprep or a maxiprep. In these purifications, you generally lyse the...

15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...and are described in Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

16. 9 tips for a successful postdoctoral experience

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    ...early on. Once your system is working, you should maximize data production with the end goal of having sufficient...

17. Plasmids 101: Using Transposons in the Lab

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    ...mammalian cells without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac...

18. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    ...cells were transfected in 96-well format using an Amaxa Shuttle device; 5 days after the transfection, the...

19. Special Delivery: Fluorophore Targeting for FRET Studies

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    ...FRET acceptor. These experiments will define the maximum and minimum energy transfer values you can expect...

20. A Needle in a Base-Stack: Cas9 Structural Biology

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    ...duplex, and it’s the reason guide sequences have a maximum length of 20 bases: nothing longer will fit. Stabilization...