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Showing: 101 - 120 of 275 results

  1. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... features of the enzymes documented that should narrow the field for a candidate Cas for your experiment... S., Wolf, Y. I., Iranzo, J., et al. (2019). Evolutionary classification of CRISPR–Cas systems: a burst...

  2. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... that template for repair. By using these two scenarios, you can either knock out a gene or knock in a...chance they will bind that sequence. That’s the revolutionary aspect: TALENs take a technology that was difficult...

  3. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...replicative origins that can be used for broad or narrow range of microbial species. These also contain ...cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering of bee gut...

  4. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...to easily discern the proteins in your assay.  Narrowing the field After working your way through the above...number of options. If this is the case, you can narrow them down in a variety of ways. First, review the...

  7. Plan Your Experiment

    Type
    Collection
    ...specific genetic manipulation can be a good way to narrow down which reagents are appropriate for a given...

  8. Deisseroth INTRSECT Collection

    Type
    Collection
    ...opens in a new window) Rovira-Esteban L, Gunduz-Cinar O, Bukalo O, Limoges A, Brockway E, Müller K, Fenno...

  9. CRISPR in the Clinic

    Type
    Blog Post
    ...early-phase clinical trials and have shown promising preliminary results (Dimitri et al). CRISPR engineering of... Cas family) as part of a cocktail therapy for urinary tract infections. The Cas3 enzymes target several...

  10. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...from more distantly related species and/or use narrowly specific secondary antibodies rather than generally...probing multiple targets in parallel, use the most narrowly specific secondary antibodies available to minimize...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...chemistry is complex and this work requires an interdisciplinary team.  Day two of the conference was kicked...of years of common ancestry with worms before evolutionary divergence. Nikolaus Rajewsky from the Max Delbruck...

  12. Of Myc and Men

    Type
    Blog Post
    ...Munro, et al. 1986; Munro and Pelham, 1987). They narrowed down the epitope recognized by Myc1-9E10 down ... monoclonal antibody with high specificity. (2) Narrow down the specific epitope that your antibody binds...

  14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...transduction of both the CNS and PNS.That is until the Gradinaru Lab at Caltech stepped up to the challenge. Find...therapy treatments for neurological diseases). The Gradinaru Lab has a lot going on. Here are the key takeaways...

  15. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...makers have moved away from long written proposal narratives. Now they require grant seekers to use online...demand for stronger budgets with clear budget narratives, logic models, established partnerships and collaborations...

  16. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...some microscopy enthusiasts to start a microscopy seminar to connect the imaging community and to create ...for lunch, make a point of talking to them at a seminars, or even set up regular meetings. Meeting people...

  17. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... courses of treatment with antibiotics These scenarios expose bacterial populations to antibiotics at...counter-intuitive considering the other three scenarios, but a short treatment regiment might wipe out...

  19. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...CBE. Base editors for the plant genome These preliminary studies demonstrated successful base editing ...than one genomic site simultaneously.     These binary vectors could be used to generate stable genome...

  20. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...replicate in, and persist in. Some plasmids have a narrow host range, while others have a broad host range...2009). Figure 1: Idealized examples of a narrow host range plasmid with only one ori and a broad...
Showing: 101 - 120 of 275 results