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We narrowed to 172 results for: plasmid for e coli

Showing: 101 - 120 of 172 results

  1. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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    Blog Post
    ...nights :D) engineering E. coli to produce biofuels in Pamela Silver’s Lab (plasmid page here). Now I spend...helping scientists share information and, of course, plasmids. My primary duties are to manage this very blog...interesting than that will simply get people ordering plasmids. The need to keep content coming also keeps me...talking to researchers about their work, picking up plasmid deposits, and trying to learn ways Addgene can ...

  2. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ...underrepresented plasmids (plasmids that were lost or “dropped out”) and overrepresented plasmids (plasmids that ...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds...library contains many plasmids, care must be taken to ensure that all of these plasmids are amplified and ...diversity. If the various plasmids in the library are differently sized, smaller plasmids may be amplified preferentially...101 Library amplification seems straightforward: E. coli bacteria are transformed with the library DNA, ...hundreds, thousands, or even a million plasmids. These libraries are some of Addgene’s most exciting and versatile...harvested, much like one would prep an individual plasmid. However, lots of library DNA is needed for screening...

  3. Genetic Code Expansion

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    Collection
    ...tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG Huiwang Ai 68292 SepOTSλ SepRS9 E. coli Sep Bacterial TAG...synthetase E. coli Mammalian TAG Simon Elsaesser 140019 pAS_4xEcoLeuT(CUA)_AnapRS AnapRS E. coli 3-(6-acetylnaphthalen...leucyl-tRNA-synthetase E. coli photocaged cysteine Mammalian TAG Huiwang Ai 73544 pEvol-pAcFRS.2.t1 pAcFRS.2.t1 E. coli...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ...Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Abhishek Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells. You should ...

  4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post
    ...engineer 14 chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed, asPink, spisPink, scOrange...at the cells on the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, ...colonies to determine which cells have taken up the plasmid. Of course, other follow up methods like sequencing...chromoproteins. In some cases, cells containing high-copy plasmid-borne chromoproteins lost color overnight in liquid...problem. There is no single “ideal” chromoprotein plasmid that combines the most desirable features: intense...Chromoproteins for allelic exchange Figure 3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus...sequential homologous recombination events: The plasmid carrying the desired genetic modification integrates...

  5. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ...proteins. She also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...Nathaniel Roquet from the Lu Lab at MIT developed plasmid based systems that report the order in which specific... plasmid...

  6. Plasmids 101: Inducible Promoters

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    Blog Post
    ...Two-Component Systems for E. coli Browse other blog posts from our Plasmids 101 series Resources on Addgene.org...Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable...Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...use light to regulate transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI...Inducible Systems at Addgene Cre-lox Guide and Plasmids     ...

  7. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ... If there’s a plasmid or AAV tool that you can’t find, you can always suggest a plasmid to us, request...you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply your ...want to get into the field. Find Optogenetics Plasmids for Use in Neuroscience Research Neuroscience ...400 who signed up for our newsletter and a free plasmid) were very excited about our new viral services...Service Find Retrograde AAV Browse Optogenetics Plasmids ...

  8. Hot Plasmids - February 2022

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    Blog Post
    ...2021 Plasmids are used to express 10, 15, 20, 30, 40, 50, 60, 80 and 100 kD proteins in E. coli, either...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'...Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective alternative...kD proteins or 20, 40, 60, and 80 kD proteins. Plasmids containing 150 or 250 kD proteins are also available...

  9. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Browse our Plasmids 101 blog posts Read blog posts on plasmid cloning Read about different plasmid elements... not work. Check the plates! Streak on them an E. coli  strain known to be sensitive to that antibiotic...to the backbone plasmid; a starting point could be a molar ratio of 1:2 (plasmid:insert), but this parameter...colonies screened might not contain the correct plasmid. There’s a lot that can go wrong! With all the ...cloning project, and hopefully obtain your coveted plasmid with no substantial delays. Design, design, design...spend some time outlining the construction of the plasmid and all the steps you will have to take. Decide...you chose, and verify that the sequence of the plasmid you are working with is correct. It's also best...

  10. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here.  Listen to the hot plasmids episode! New, highly...hundred SH3 domain containing plasmids available through Addgene. These plasmids can be used to carry out ...function. RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs target...dsRNA necessary for RNAi. The Vellai lab’s new plasmid, T444T, contains T7 terminators adjacent to the...to increased RNAi efficiency. Find the T444T plasmid here! Strum A, et al. Nucleic Acids Research. 2018...

  11. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post
    ...hold the plasmid together, even if there are gaps or flaps remaining. This incomplete plasmid looks like...robust system of homologous recombination found in E. coli, SLIC is a cheap, standardized, and rapid multi-part...cloning uses short sticky ends to insert genes into plasmid backbones, base pairing between these ~4 base overhangs... overhangs isn’t strong enough to hold a plasmid together through transformation, so the cloned vector...longer single-stranded overhangs can hold a nicked plasmid together long enough to get it into cells and let... of LIC is often limited to specially designed plasmids. Sequence- and ligation-independent cloning: A...fixing up the “damage” and producing a complete plasmid. Besides being compatible with any vector, the ...

  12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...has been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        AAV delivery... Figure 1: The base editor is split into two plasmids and co-transfected into cells for reconstitution...overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion...channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed...

  13. CRISPR in the Clinic

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    Blog Post
    ...infections. The Cas3 enzymes target several strains of E. coli and lethally chop their genomes into many, many...ijms24021563 Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook CRISPR History ...Hussein M, Molina MA, Berkhout B, Herrera-Carrillo E. A CRISPR-Cas Cure for HIV/AIDS. Int J Mol Sci. 2023...

  14. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ... demonstrated in 2005 that proteins fused with E. coli dihydrofolate reductase (eDHFR) could be labeled...resources References Bottanelli, F., Kromann, E. B., Allgeyer, E. S., Erdmann, R. S., Wood Baguley, S., Sirinakis...Allgeyer, E. S., Zhong, S., Thompson, A. D., Lowe, N., Butler, R., Bewersdorf, J., Rothman, J. E., Johnston...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...Schepartz, A., Baddeley, D., Toomre, D. K., Rothman, J. E., & Bewersdorf, J. (2016). Two-colour live-cell nanoscale....chembiol.2019.01.003 Gallagher, S. S., Sable, J. E., Sheetz, M. P., & Cornish, V. W. (2009). An In Vivo...

  15. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ...Standards Synthetic Photobiology: Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources...

  16. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post
    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent... fluorescence-activated cell sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey...PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors.” Curr...nucleotide aptamer they called Broccoli. As a product of SELEX and FACS, Broccoli is inherently more stable...new RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian...mammalian cells. They’ve also dimerized Broccoli to further enhance its biosensor capabilities (see Fig...et al. “In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies....

  17. CRISPR Pooled gRNA Libraries

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    Collection
    ...33,170 Bikard Lab E. coli Genome-wide Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 92,...Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ∼5 21,... Zhang E. coli Genome-wide Inhibition Library 113134 — 113138 113146, 113147 Inhibition E. coli Chong ...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 (2 plasmid) Knockout Human Doench and Root 3rd... 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...

  18. Rinehart Lab Phosphoprotein Reagents

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    Collection
    ...SepOTSλ Plasmid 68291 SepOTSκ Plasmid 68290 SepOTSι Plasmid 68289 SepOTSθ Plasmid 68288 SepOTSη Plasmid 68287...S218TAG Plasmid 68300 MBP-MEK1 Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297...GFP Q157TAG Plasmid 68296 GFP S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ...68287 SepOTSζ Plasmid 68286 SepOTSϵ Plasmid 68285 SepOTSδ Plasmid 68284 SepOTSγ Plasmid 68283 SepOTSβ Plasmid....ΔA.Δserb.Amp Plasmid 68305 Beta lactamase S68TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68301 MBP-MEK1...SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus plasmid and is ... other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express...

  19. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., Tolar, J., Meissner, T. B., Chaikof, E. L., Osborn...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...prime editors with RT domains derived from the E. coli Ec48 retron RT and the S. pombe Tf1 retrotransposon...for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration...-01025-z Doman, J. L., Pandey, S., Neugebauer, M. E., An, M., Davis, J. R., Randolph, P. B., McElroy, ...Solley, S. C., Gilbert, L. A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R., & Adamson, B. (2024)...

  20. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post
    ...methionine (i.e. the plasmid complements the deletion). Find the Plasmids Here Plasmid features enable multiple... that allow students to propagate the plasmids in either E. coli (ori and ampR) or S. cerevisiae (2µm ...the overexpression plasmids. Finally, students determine if the presence of the plasmid corrects any known...diverged. Tips on using the Pathways Over Time plasmids The plasmids are used as part of a semester-long research...less-well studied organism into overexpression plasmids. Students next transform deletion strains from...phenotypic abnormalities due to the deletion (i.e. the plasmid complements the deletion). If the phenotype is ...cerevisiae met deletion strains with overexpression plasmids carrying MET/Met genes from either S. pombe or...
Showing: 101 - 120 of 172 results