... of tumor antigen. I asked Howarth to tell us more about SpyLigase, its development, and its potential... different approaches to improve CTC capture, but I hope that our antibody tentacles may have an impact...plasmids that may well be of use to others to Addgene. I am a big fan; thanks for the T-shirt! References:...

... this event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both...irreproducibility with some solutions based on what I’ve learned. I hope you can apply some of these solutions to your...align better with an initial hypothesis. To be clear, I don’t think that the majority of researchers do these...whether the ultimate results were positive or negative. I can feel you rolling your eyes, but even if this ideal...plasmid repository 49:45 0 1:18:45 - Reagent Development - Steven C. Almo, Institute for Protein Innovation...

...this PI looking for in a new researcher?,” “How do I convince the PI to choose me?” We asked three leading...how it will be before you take the position. Am I choosing the right mentor? Make sure your advisors...neighbors during the interview process. What if I want a non-academic career path?  If you are considering...source software, hardware, wetware, technology development, etc). Second, the potential hire visits the ...identifying a lab that fits both work style and development needs. These two factors will provide a solid...

...defeat it we need to attack it from all fronts!”  Here I aim to compile a few highlights from the conference...Methods to study autophagy can be found here. Fun fact: I also learned that Professor Kroemer often functions...from the poster sessions During the poster session I got a chance to talk with Dr. Friedmann-Morvinski ...CAR T cells and PD-1 modulators. An exciting development in immunotherapy is the combination of the CRISPR...PMID: 28225754. 6. Marumoto, Tomotoshi, et al. "Development of a novel mouse glioma model using lentiviral...

...room and don’t have to keep buying more freezers. I am pretty sure that the world will go on without my...the same. Long-term lab conservation goals Lastly, I think it’s a good balance to have both short-term ...reduce your consumption in the lab: Turn off your equipment at the end of the day and when not in use. You...making dinner at night, so why leave your lab equipment on? This applies to water baths, computers, and...

...the common mode of operating.  Managing scientists I should add a few special words about managing scientists...Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own to this list. ...work, opportunities to pursue and problems to solve (I think this is true of most people, not just scientists...training or teaching others, research, and policy development. Every worker at every level does better with...

...Cepko to learn about her mentoring style. How can I find out if an advisor is a good mentor? You have ... more resources to help you choose a good mentor, I have provided a downloadable PDF at the end of this...scientists think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad mentor can be...practice successful grant writing, lab budgeting, equipment acquisition, public speaking and personnel management...

... Open source hardware builds   Since I started my PhD, I have been convinced that most microscopy ...a number of new platforms (such as openUC2, which I am a co-founder of), that aim to support the community...taking these limitations into account in the development process. Widely used, commercially available ...Squid: Simplifying Quantitative Imaging Platform Development and Deployment." bioRxiv. 2020. Diederich, B....

...   3. Start something In almost every career talk I present, I’m asked what Addgene “looks for” when hiring... The online dictionary gives two definitions that I like: “the ability to assess and initiate things independently...start? 4. Do informational interviews (on a schedule) I hope you all know by now that people get jobs at least...wait for it to be over to work on career skills development. Back in March, we posted these 25 great tips...

...consider my first challenge when I started working with AAV vectors. I wanted to use an AAV vector to express...effort to test and optimize our vector - overall, I spent two years testing and reformulating our AAV ...from people trying to answer the exact same question I was: What AAV vector is best for my specific system...Vectors. Molecular Therapy - Methods and Clinical Development, 18(September), 98–118. https://doi.org/10.1016...

...editing techniques is its ease of use.  “I was really excited when I saw these two papers,” said the University...conditions. Remaining hurdles in CRISPR biology “I think it is absolutely possible to use CRISPR to cure... It is speeding the process of the discovery/development of genetic targets and RNA therapies. [6]. In...

... able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now we can with Cas13b/ADAR ...from the REPAIR (RNA Editing for Programmable A to I Replacement) system. Like RNA targeting systems, RNA... to make it easy for you to keep up with new developments (and, of course, find plasmids that will be ...CRISPR History provide a great overview of the development of CRISPR into a varied research toolbox, as ...Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

...CRISPRa, the Day lab singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary...hundreds of differentially expressed genes between Bdnf I-overexpressing neurons and Bdnf IV-overexpressing ...Among the upregulated genes common between the Bdnf I and Bdnf IV overexpression variants were other immediate-early...University of Rochester Medical Center. During development, complex genetic programs specify and assemble...technological gap, a trio of recent papers describe the development of CRISPR activation (CRISPRa) tools for neuroscience...recently circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH) transgenic mouse (...regulates neuronal physiology by promoting the development of neurons and synapses as well as long-term ...

...phylogenetically classified as the more complex Class I systems that contain multiple subunits, but the more...its ancestors. They classified the system as Class I, Type III-E, and then used this information to identify...03886-5. Epub 2021 Sep 6. PMID: 34489594. Eleonora I. Ioannidi, Matthew T. N. Yarnall, Cian Schmitt-Ulms...and cellular biology, this happens through the development of tools that exploit and expand our current ...which we're featuring in today's blog. Cas7-11 Development and Discovery As part of their quest to find,...Cas systems opens up the potential of further development of low-toxicity RNA editing tools.   Fig...embryonic stem cells and glioblastoma cells. PASTE Development and discovery The first generation of CRISPR ...

...protein after a vegetable substitute for spaghetti? I actually couldn’t find the specific origins of the...does not form properly (Azplazu and Frasch, 1993). I could not find the exact origin of this gene name ...contains bagpipe from the Deplancke lab. Conclusion I hope you enjoyed this tour of Addgene’s new conference...primarily involved in development. These genes are known to play a role in neural development, sex determination... a transcriptional co-repressor important in development. Groucho family proteins are found in flies, ...Hedgehog, an important regulator of embryonic development The largest conference room at Addgene is named...rather to an extremely important gene in animal development. To understand where sonic hedgehog got its name...

...how does NgAgo stack up thus far? A few weeks ago, I was going through NgAgo google forums where people... dig more into what researchers felt about NgAgo, I conducted a survey and asked researchers if they had...people are having difficulty with the NgAgo system. I encourage you to share your results and protocols ...More time is required for its optimization and development before it can truly be pitted against CRISPR ...Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering...

...invisible information into actionable signals. Let's say I wanted to detect every primary metabolite in a cell...sensor domains for everything you could imagine. That I think is the next priority; we need the keyboards ... programming language is put into practice? Endy: I think it's important to understand that these platforms...with a publication in Science describing the development of “transcriptors,” transistor-like digital genetic...engineered RNA switches, but we've not yet seen the development of enough sensor domains. Ultimately, we'll need...

...translocation event is generated at the loxP sites. How can I use Cre-lox? The Cre/lox system is a well-established...successfully introduce Cre into cells of interest. What if I need two separate recombination events? One potential... 1. Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. Sternberg, N. and...cell or tissue types, during certain stages of development, or by making the Cre inducible (such as with...

...stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to clone from the...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...

... and experimental limitations and considerations? I recently spent two months in the lab of Antonello ...behavioral experiments. I’m not an electrophysiologist so I cannot speak directly on this topic but, in numerous...neural pathways involved in addiction (such as the lab I work in), an obvious behavioral experiment to test...injections until they are accurate and reproducible. I also highly recommend spending the money on a high... one of the key scientists who pioneered the development of optogenetics, has genetically manipulated ...nrn3171. PMID: 22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review...