We narrowed to 132 results for: primer

1. 5 Tips for Troubleshooting Viral Transductions

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   Blog Post

   ...Adenovirus Associated Virus New to Virus? Check out Our Primer for Beginners! Learn All about Lentiviral Vectors...

2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   Blog Post

   ...and how they work, watch Endy's 10-minute YouTube primer on BIL gates.) Addgene: Why is it so important ...

3. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   ...determining titers by qPCR, we recommend using primers targeting the viral genome or inverted terminal...

4. Droplet Digital PCR for AAV Quantitation

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   Blog Post

   ... plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix and...

5. Fluorescent Proteins 101: Aptamer Fluorophores

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   Blog Post

   ...half a century; the Addgene blog has an excellent primer and tips from experts on FRET techniques. By combining...

6. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...process. Either SYBR green technology or a TaqMan primer/probe set can be used and samples are quantified...

7. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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   Blog Post

   ...videos can be especially useful when designing new primers to clone your sequence of interest into Level 0...

8. Filming Science Videos in the Age of Social Distancing

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   Blog Post

   ... it worked. We pulled it off! Check out the PCR Primer video! Learn: Producing videos like this would ...

9. Important Considerations in Optogenetics Behavioral Experiments

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   Blog Post

   ...Central PMCID: PMC3032981. Resources at Addgene A Primer on Optogenetics: Introduction and Opsin Delivery...

10. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

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    Protocol

    ...the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence ...

11. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post

    ... of the improvements: SHERLOCKv2 uses far less primer in the pre-amplification step allowing for greater...

12. Plasmids 101: Biotinylation

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    Blog Post

    ...Polymerase Chain Reaction (PCR) by using a biotinylated primer. Biotin can also be enzymatically incorporated ...

13. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post

    ...rate of the organism of interest, from the day primers arrive, the desired chromosomal modification can...

14. Plasmids 101: Restriction Cloning

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    Blog Post

    ...you should send it for sanger sequencing using primers that will allow you to read over the insert. See...

15. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    ...post, Plasmids 101: Cre-lox, if you need a quick primer on how Cre-lox recombination works. Advances in...

16. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...inserts following colony PCR of 10-20 colonies using primers bordering the multiple cloning site of the vector...

17. Plan Your Experiment

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    Collection

    ...qPCR Knockouts Activation/interference Needs good primer design Western blot Knockouts Activation/interference...

18. 22 Hot Plasmid Technologies from 2014

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    Blog Post

    ...select the appropriate pair of vector and gene primers from Table 2 of the associated publication which...of sequence homology between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and...

19. Molecular Cloning Techniques

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    Guide

    ...Complementary overhangs are built into the PCR primers for the insert, based on the destination vector...Additional Resources Addgene Webpages Sequencing Primers Modular Cloning (MoClo) Guide Choosing Your Perfect...

20. Which Fluorescence Microscopy Technique is Best for Me?

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    Blog Post

    ...University.     Additional Resources Online Microscopy Primer on the Molecular Expressions Website Other major...