Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 151 results for: primer

Showing: 101 - 120 of 151 results

  6. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics    ...

  10. Molecular Biology Reference

    Type
    Guide
    ... plasmid. For commonly used primers check out Addgene's sequencing primer list. Working with Plasmids ...enzyme, the template DNA to be copied, and a primer. A primer is a small piece of DNA, approximately 18-...select or sort the cells by visualization or FACS). Primer Binding Site A short single-stranded DNA sequence...amplification or DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert...know some of its sequence to design a effective primer. Sanger sequencing chromatogram Sanger sequencing...replication, the Sanger sequencing reaction begins when a primer binds to its complementary DNA and the DNA polymerase...approximately 500-1000 bases downstream of the known primer region with very few errors making it an efficient...

  11. Molecular Biology Reference

    Type
    Guide
    ... plasmid. For commonly used primers check out Addgene's sequencing primer list. Working with Plasmids ...enzyme, the template DNA to be copied, and a primer. A primer is a small piece of DNA, approximately 18-...select or sort the cells by visualization or FACS). Primer Binding Site A short single-stranded DNA sequence...amplification or DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert...know some of its sequence to design a effective primer. Sanger sequencing chromatogram Sanger sequencing...replication, the Sanger sequencing reaction begins when a primer binds to its complementary DNA and the DNA polymerase...approximately 500-1000 bases downstream of the known primer region with very few errors making it an efficient...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...expressed is amplified using a gene-specific forward primer containing the desired restriction site (the MCS...has several options) and a pUCX-specific reverse primer containing the missing codons from the antibiotic...if a small contaminating PCR product formed from primer dimers and containing the missing codons from the...resistance gene is inserted. It is important that primers be designed to minimize the possibility of dimer...
Showing: 101 - 120 of 151 results