We narrowed to 130 results for: primer

Ready to produce rAAV in your own lab? This 101 post can help!

Find tips and tricks for using digital droplet PCR to quantify your AAV titers.

Get tips for producing, purifying, and tittering AAV, a tick but useful viral vector of choice for many people working to express genes in mammalian cells.

Learn about aptamer-based fluorphores and how they can be used to brighten up your experiments.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Learn about the best tips and tricks for modular cloning (MoClo) of plasmids!

A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations.

Get a behind-the-scenes look at how we've adapted video production during COVID-19.

Learn how CRISPR-based diagnostic systems can easily detect nucleic acids of choice with high sensitivity, specificity and portability.

Joining the popular sacB counterselection method is a new suite of toxin-based allelic exchange vector that can be used in many proteobacterial genomes.

Learn how biotin is used in a variety of molecular biology experiments and makes it easier for researchers to label and purify macromolecules of interest.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

Learn about a useful technique to screen phage genomes for host binding proteins and discover new research avenues.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Get tips on choosing the best fluorescence microscopy technique for your next imaging experiment.

Learn how to target donor and acceptor fluorophores for FRET experiments.

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

Learn about the basics of molecular biology, including molecular genetics, plasmids, sequencing, and more.