Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 535 results for: val

Showing: 101 - 120 of 535 results

  1. Antibodies 101: Isotypes

    Type
    Blog Post
    ...means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant domain replaces... is unknown. It has a monomer structure with a valency of two and has no subclasses. IgG IgG is the most...subsequent time. It is expressed as a monomer with a valency of two. There are four IgG subclasses, numbered...subclasses and they are expressed as monomers with a valency of two.  IgA IgA antibodies are found in blood,... common antibody after IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus.... This isotype is most commonly a dimer with a valency of four. There are two IgA subclasses: IgA1 and...

  2. Technical Design of a Western Blot

    Type
    Blog Post
    ...recommend using an antibody validated for your target in your specific assay. If validated antibodies aren't available... different than the validation assays, we recommend performing your own validation assays. If you’re using... the manuscript and supplementary figures for validation data, or follow the references to find the original...original publication and ensure that it includes validation data relevant to your application — and double-check...significantly from lot-to-lot, and have to be re-validated each time a new lot is purchased. Monoclonal antibodies...manufacturer, each rAb in a mixture can be individually validated. ‌rAbs are not as commonly available as monoclonal...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...nucleic acids, or glycans. Fluorophores can also be covalently attached to self-labeling proteins. Some common...a genetically encoded enzyme variant tag that covalently reacts with a small substrate ligand motif attached..., maintaining intensity at ~85% of its original value after 60 minutes of illumination, highlighting its...(Sridharan et al., 2014). It's also crucial to evaluate how the addition of fluorophores affects the functional... or the degree of labeling (DOL), and thorough removal of unconjugated dye molecules are critical for ...filtering settings of the equipment. Systematic evaluation of commercially available dyes with different...Kurumizaka, H., Nozaki, N., & Kimura, H. (2014). Evaluation of chemical fluorescent dyes as a protein conjugation...

  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...https://doi.org/10.1126/science.abf9302     Validated recombinant antibody toolbox for aminoacyl tRNA... (aaRS) have been created, characterized, and validated by the Gräslund lab. Recent findings suggest that...inflammation and autoimmune diseases, so these validated antibody tools could be used to study the role...slow off-rate binders. The best candidates were validated by IP-MS using HEK293 cell lysate where the antigens...system. Image from Cheah et al., 2021.   After validating this method through monitoring GFP degradation...Dimidschsteind lab provide expression selectively in Parvalbumin containing cortical interneurons. We now provide...

  5. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...non-homologous end joining (NHEJ) or HDR. EGFP validation of sgRNAs: Can evaluate component delivery and sgRNA efficacy...Target DNA reporter system: Can evaluate component delivery with validated tools.  Special thanks to Joel...delivery be more suitable? Determine how you will evaluate the outcome: Will you be detecting insertions/...testing tools: Traffic Light Reporter System: Can evaluate lentiviral component delivery as well as genome...

  6. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...May include validation data CiteAb Any Any Search engine May include validation data  Biocompare...help: Antibody data repositories, which share validation data, and antibody search engines, which allow... you're looking for an antibody, especially a validated antibody, we recommend using antibody search engines...from many vendors, while data repositories share validation and experimental data to help you decide if the...

  7. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...aims. 2. Then make decisions based on financial values. All too often, we see early investigators making...can help you compare the monetary and empirical value that helps you determine whether or not to proceed...finances, instead of solely discussing numerical values, try to keep conversations focused on research ...working within their limitations. 3. Always provide value to others This is a non-profit organization. Therefore...Therefore, collaboration and community is the ultimate value proposition. By preparing a budget before hand, ...the questions we mentioned previously.  There is value in the process of creating a budget; you can share...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...antibodies that have been validated in IF applications (find IHC validated or ICC validated antibodies from Addgene... or be prepared to run some extra controls and validate the antibody yourself. If you can’t find a suitable... combination of colors you like. Controls and validation Can’t forget the controls! The necessary control...., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M., & Weissgerber, T. L. (2021...

  9. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...homepage is still important. It needs to express value, clearly communicate exactly what a site offers ...and navigation, we also wanted to emphasize the values that make Addgene unique: community, sharing, and... easy and fun every day.  With these three main values we set out to radically redesign our homepage to... and made a commitment to reinforcing the three values with each element. We also set out to make sure...to be friendly, personable, fun, and promote our values of community and sharing.  One big feature upgrade...

  10. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ... for validation data on IBEX reagents.  mAb3D atlas validated antibodies: A database of validated antibodies...These links can help you find experimental and/or validation data on molecular biology tools.  Addgene Data...Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors.  Caspedia: A data repository...

  11. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...immobilization for kinetic analysis Proteolytic removal of the fusion protein could provide a more relevant...second immobilization method. One possibility is covalent modification of the purified target protein with...and neutravidin) is for all practical purposes covalent. Therefore, protein immobilization through biotin-avidin...amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids. Unfortunately... the erythropoietin receptor and subsequently evaluated interactions with mutant and wild-type erythropoietin...

  12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...reagents If you want to label intracellular proteins covalently with FRET acceptors, then biarsenicals are a ...The utility of these reagents is that binding is covalent, so free dye can be washed away. In addition, ... define the maximum and minimum energy transfer values you can expect, thereby establishing the dynamic...design lends itself to creativity and innovation, valuable commodities in any NIH grant application! Using...Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling calibrated FRET distance measurements...Stephen R. Adams, and Roger Y. Tsien. "Specific covalent labeling of recombinant protein molecules inside...

  13. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... administration, and dose (Lu et.al., 2020).  Evaluating a drug’s immunogenicity in-vitro helps researchers...in vitro assessment of immunogenicity can help evaluate risk of an antibody.  In silico prediction of...EpiMatrix is a commercially available algorithm to evaluate the immunogenicity of therapeutics in silico. ...of in vitro prediction of immunogenicity is to validate the findings from the in silico assessments, as...prediction algorithms fail to capture. In vitro evaluation of ‌therapeutic immunogenicity is typically performed...

  14. Antibodies 101: ChIP

    Type
    Blog Post
    ... best to either select antibodies validated for ChIP or to validate your antibody on crosslinked proteins...for exploring these interactions, and can give valuable insight into DNA expression and protein interactions...for the first time, look for one that has been validated in multiple assay types. Remember that ChIP uses...uses native proteins (so antibodies validated only for denatured proteins may not be a good choice) and...

  15. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...completely missing in women. Bicuspid aortic valve (an aortic valve that has two leaflets, instead of three...murine solute channels, the team will be able to validate their findings in mammalian cells with both human... are two heart defects that are 50 times more prevalent in women with TS. “The goal of our study is to...understand why women with TS develop bicuspid aortic valve at a rate that is 50-100 times higher than women...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...performed during your harvest. Validation Recombinant antibodies should be validated to ensure they bind their...antigens specifically. Possible applications for validation include western blot, immunohistochemistry, and...produced, harvested, purified, quantified, and validated, it’s ready for use! You can learn more about ...coefficients of monoclonal antibodies with experimental values as measured by the Edelhoch method. Int J Biol ...

  17. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...are critical to stem cell survival. Knowing which genes influence the survival of our cell culture is the...promoters, insulators, and more. I then identified valid gRNA sequences without off-target effects for the...CRISPR 101 Learn about CRISPR Delivery Methods Validate Your Genome Edit Resources on Addgene.org Find...Your Research Check out Our CRISPR Guide Find Validated gRNAs ...

  18. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...infected with Cas9. Validating the Library The CRISPR knockout library was validated via a growth screen...are used to call hits from the growth screen, p-values were less significant than when non-targeting guides...guides are used as controls. Less significant p-values lead to more false-negatives results (i.e. failing...library resulted in both increased and decreased p-values of known ricin regulation genes compared to results...

  19. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... sgRNA Addgene may carry a validated sgRNA for your target gene.  Validated gRNAs are a great bang for... freely available sgRNA design programs. Find Validated gRNAs for Your Next CRISPR Experiment Cas9 expression...Mammalian Expression Systems and Delivery Methods”. Validating your edit Once you have delivered Cas9 and a ... found in our blog post entitled “CRISPR 101: Validating Your Genome Edit”. Is Cas9 not your Cas of choice...

  20. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ...is a values-driven nonprofit. Recent remarks and actions by X’s leadership conflict with our values. For...monitoring, and resharing content on X.  Addgene deeply values our community and recognizes the impact of this...allows us to remain aligned to our organizational values.  To preserve the knowledge, conversations, and...
Showing: 101 - 120 of 535 results