...Stanford University. Sex and gender are critical components of biological research that are often forgotten...cost at this early stage of research is minimal compared to that of human clinical trials. And, as Janine...studies that draw from a body of research that is incomplete (because sex was not analyzed) may harm humans...

...Cloner, and Snapgene), and save these to a shared computer or server. This will ensure that future members...more than two sequencing reactions. In the more complex cases, we sometimes have to design new primers ...saves us the step of purifying the DNA and gives comparable success rates and sequence quality, which is ...

...response against AdV. Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene...negligible toxicity. However, their production is more complex and the presence of helper virus contaminants have...amplification.*Pro-Tip*: The cells will become completely confluent and the media will turn yellow. Do ...

...clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore, CRY2clust...strain can be rescued by the xanthine produced via complete demethylation of caffeine. For example, the Barrick...a 1.2-fold greater extinction coefficient when compared to sREACH. Find the FLIM-FRET acceptors here. ...

...allowing us to do up to 96 transformations in one run compared to doing about 50 transformations in the same ...increased plasmid demand it was too time consuming to complete this process by hand and we welcomed yet another...liquid handler can fill 400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in...

...choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety of ...proteins interacting. Experiments tend to be equally complex with users trying to probe multiple targets in ...

...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...for the past three years. pgEd is a small team comprised of individuals from diverse backgrounds and disciplines...social context. This “general public” audience comprises the taxpayers who fund much of scientific research...

...machinery.   Well, I’ve got some good news: complete DNA demethylation can be achieved by dCas9 alone...too.  High dCas9 and gRNA expression are key for complete DNA demethylation – tight binding is crucial for...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...

...share, remix, store, and track these essential components of the scientific toolbox. What are Recombinant...subsequent purification.  Academic laboratories and companies have already begun creating plasmids encoding ...NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal Antibody Sequences...

...cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted expression was...retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in mouse, monkey...targeted cells in monkey and human retinas. By comparing the performance of promoters across these three...