Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 878 results for: cel

Showing: 591 - 600 of 878 results

  1. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP and represses... Because transcription machinery differs between cell types or organisms, promoters are similarly variable...variable. Bacterial promoters only work in prokaryotic cells and typically only in the same or closely related... were derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique...

  2. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...aiming to dedifferentiate mouse brain cells into neural stem cells. I was quite unsuccesful (although I...versatile means of delivering genes to many different cell types with minimal immunological effects. AAVs are... used in experiments targeting genes to specific cell types in the brain, mapping neuronal networks, and...

  3. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ... nerve and muscle function, hormone release, and cell signaling throughout the body. Because calcium levels...used simultaneously to monitor or control different cell types or signals in the brain. For this to work,... researchers can image multiple brain regions or cell types at once using existing indicators. For example...available through Addgene. Whether you're working with cell cultures, slice preparations, or in vivo imaging...

  4. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ... results, but also confirm expression in target cells. While the various AAV serotypes can be used to ...Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in the brain, and holds promise... systems such as Cre-lox allow for more accurate cell and tissue targeting. While promoters and AAV serotypes...allowing you to further restrict gene activation to cells expressing both Cre and GFP. The GFP dependent FLPo...

  5. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...co-delivering different AAV particles into the same cells in vivo? A: This will be highly dependent on vector...particularly one that can target the epithelial cells in the blood-brain barrier?   A: For liver, we recommend...worth investigating for both Q6 & Q7.For epithelial cells of the blood brain barrier we would currently recommend...and to all our participants for their time and excellent questions.    Additional Resources on the Addgene...

  6. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...see the value to the scientific community in accelerating the Viral Service. With their support we are...helps keep our focus on assisting scientists and accelerating research and discovery, while also pushing us...take on this project? Addgene’s mission is to Accelerate research and discovery by improving access to... facet of a project this big. Maintaining our excellent service and accuracy involves caring and careful...

  7. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...also gives cells antibiotic resistance to neomycin so that you can select the population of cells with the...Only a small percentage of the proteins inside a cell have a commercial antibody against them. There’s...antibody. It’s an epitope not found in human or other cells. It was made artificially. That means you aren’t...

  8. Deep Dive: qPCR

    Type
    Blog Post
    ... that your reference genes are suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle...reference genes to save on plate space. For many cell lines and tissue types, appropriate reference genes...at determining the best options for a particular cell or tissue type (such as here, here, or here).  If...for analyzing qPCR data, most with plug-and-play Excel spreadsheets available for easy analysis. These ...your data  Once you’ve done the work to produce excellent and reliable qPCR data, you’ll of course want ...

  9. Addgene: The First Twenty Years

    Type
    Blog Post
    ...amazing years in front of us. So in our last celebratory moment, we wanted to walk through some of our...Facebook and Gmail, were launched. And Melina Fan, a cell biology graduate student, was frustrated by the ...to receive the Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also ...now our Sr. Director of Production) pie-fully celebrating Addgene's 10 year anniversary.   Expanding...

  10. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...product, the transformation may not result in any cells, or all the colonies screened might not contain ...verify the strain and the efficacy of your competent cells (when they get old, they lose competency). If all... look at your experimental design. Full lawn of cells. The antibiotic in your plate might not work. Check...acknowledged in hundreds of publications including Cell, Nature, and other journals.   Paolo Colombi is ...
Showing: 591 - 600 of 878 results